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Preparation method and new application of aconite polysaccharide

A technology of aconite polysaccharide and use, applied in the field of aconite polysaccharide and its extraction and purification

Active Publication Date: 2019-09-24
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the material basis of aconite warming and tonifying yang remains to be studied, and there is no research on the relationship between aconite polysaccharides and aconite’s effect of tonifying fire and supporting yang

Method used

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  • Preparation method and new application of aconite polysaccharide
  • Preparation method and new application of aconite polysaccharide
  • Preparation method and new application of aconite polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Extraction and purification method of aconite polysaccharide of the present invention

[0030] (1) Degrease Sorting and removing impurities from the traditional Chinese medicine Fuzishengfu Tablets, crushed through a 20-mesh sieve with a mechanical pulverizer, took 100g, added 1000ml of 95% ethanol, and extracted at a temperature of 80°C for 3 hours, filtered, and the filter residue was evaporated to dryness naturally ethanol.

[0031] (2) Extraction Add 1500ml of purified water to the degreased filter residue at a temperature of 90°C, extract twice for 2 hours each time (solid-to-solid ratio 1:15), filter with four layers of gauze, and combine the filtrates twice.

[0032] (3) Concentration and alcohol precipitation The filtrate was concentrated under reduced pressure to 10% of the remaining solution, transferred to a beaker, cooled to room temperature, added 95% ethanol to make the final concentration of ethanol reach 65%, and stood in a refrigerator at 4°C ...

Embodiment 2

[0036] Embodiment 2 Extraction and purification method of aconite polysaccharide of the present invention

[0037] (2) Degrease Sorting and removing impurities from the traditional Chinese medicine Fuzi Heishun Tablets, crushed through a 20-mesh sieve with a mechanical pulverizer, took 100g, added 1500ml of 95% ethanol, and extracted at a temperature of 80°C for 3 hours, filtered, and the filter residue was evaporated to dryness naturally ethanol.

[0038] (2) Extraction Add 1500ml of purified water to the degreased filter residue at a temperature of 90°C, extract twice for 1 hour each time (solid-to-solid ratio 1:15), filter with four layers of gauze, and combine the filtrates twice.

[0039] (3) Concentration and alcohol precipitation The filtrate was concentrated under reduced pressure to 10% of the remaining solution, transferred to a beaker, cooled to room temperature, added 95% ethanol to make the final concentration of ethanol reach 75%, and stood in a refrigerator at 4...

Embodiment 3

[0043] Embodiment 3 Extraction and purification method of aconite polysaccharide of the present invention

[0044] (1) Degreasing Sorting and removing impurities from the traditional Chinese medicine Aconite Radix Aconitiae, crushing through a 30-mesh sieve with a mechanical pulverizer, taking 100g, adding 1800ml of 95% ethanol, extracting for 5 hours at a temperature of 80°C, and filtering to obtain a filter residue that volatilizes naturally. Dry ethanol.

[0045] (2) Extraction Add 2500ml of purified water to the degreased filter residue at a temperature of 90°C, extract twice for 2 hours each time (solid-to-solid ratio 1:25), filter with four layers of gauze, and combine the filtrates twice.

[0046](3) Concentration and alcohol precipitation The filtrate was concentrated under reduced pressure to 15% of the remaining solution, transferred to a beaker, cooled to room temperature, added 95% ethanol to make the final concentration of ethanol reach 80%, and stood in a refrige...

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Abstract

The invention provides an extraction purification method of aconitum carmichaeli polysaccharides. The extraction and purification method comprises following steps: a, raw aconitum carmichaeli or processed aconitum carmichaeli are smashed and sieved, an obtained powder is added into 95% ethanol for warm immersion for 3 to 6h, and filtering is carried out so as to obtain a filter residue 1; b, the filter residue 1 is extracted with purified water for 1 to 3h at 80 to 100 DEG C, and filtering is carried out so as to obtain a filtrate; c, the step b is repeated for 1 to 2 times, and obtained filtrate is mixed; d, the mixed filtrate is subjected to evaporation at 55 to 65 DEG C until 10 to 20% of the mixed filtrate is left, and the left mixed filtrate is cooled to room temperature; e, 95% ethanol is added with stirring until the ethanol content of an obtained solution ranges from 65 to 85%; f, the solution is allowed to stand for 12 to 24h at 4 DEG C, filtering is carried out, filtrate is discarded, and an obtained filter residue 2 is collected; g, the filter residue 2 is washed with absolute ethyl alcohol, acetone, and ether respectively for two times, and a left residue is subjected to vacuum drying at 50 to 60 DEG C so as to obtain aconitum carmichaeli polysaccharide powder. The invention also provides the aconitum carmichaeli polysaccharides prepared via the extraction purification method, and applications of the aconitum carmichaeli polysaccharides. The extraction purification method is simple, high in extraction rate, and low in cost; and it is confirmed by pharmacodynamic experiment that the obtained aconitum carmichaeli polysaccharides are capable of preventing spleen deficiency diarrhea, and improving immunity.

Description

technical field [0001] The invention relates to aconite polysaccharide and its extraction and purification method. Background technique [0002] Aconite is a processed product of the root of Aconitum Carmichaeli Dexb, a plant of the family Ranunculaceae. Confidant cold pain, vomiting and diarrhea due to deficiency and cold, edema due to yin and cold, etc. "Tangye Materia Medica" mentioned: "Aconite, start with Shaoyang Sanjiao, the medicine of Mingmen, floating in the middle, omnipresent, spicy and hot, and it is the yang of yang"; "Materia Medica" also mentioned: "Aconite , pungent in temperature, hot in nature, rapid in fire, and everywhere, so it is the first medicine for returning to yang and rescuing the adversity"; "Compendium of Materia Medica" records: "Aconite is heavy in nature, warming the spleen and expelling cold". In modern times, aconite is often used in the treatment of chronic heart failure, shock, rheumatoid arthritis, enteritis and diarrhea. [0003] Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/02A61P15/10
Inventor 章津铭傅超美杨晓琴吴亿晗傅舒张臻游宇林彦君
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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