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Extraction and purification method of black soybean glycoprotein

A purification method, black bean sugar technology, applied in the field of extraction and purification of black bean glycoprotein, can solve the problems of low extraction rate, unstable structure and structure, etc., achieve simple extraction and purification method, facilitate large-scale industrial production, and high yield Effect

Active Publication Date: 2021-02-26
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although glycoprotein has a wide range of functions, the low extraction rate and the instability of structure and structure directly affect its development.

Method used

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  • Extraction and purification method of black soybean glycoprotein
  • Extraction and purification method of black soybean glycoprotein
  • Extraction and purification method of black soybean glycoprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The method for extracting and purifying black soybean glycoprotein of the present invention is specifically carried out according to the following steps:

[0053] S1, take black soybeans, peel them, and crush them into powder, the particle size of which is 0.1-1mm;

[0054] S2, take 1000g of black soybean powder, add absolute ethanol according to the solid-to-liquid ratio of 1:5g / mL, place it in a Soxhlet extractor, heat at 80°C for 2h, perform reflux degreasing treatment, and repeat the degreasing treatment 2-3 times;

[0055] S3, extract the degreased filter residue with 8 times the volume of distilled water at 80° C. for 2 hours, and suction filter;

[0056] S4, obtain crude sugar protein:

[0057] Concentrate the filtrate obtained in step S3 by 10 times with a rotary evaporator, add 4 times the volume of absolute ethanol at 4°C, and completely precipitate for 24 hours, filter the precipitate with suction, collect the filter residue, dissolve it in distilled water, ...

Embodiment 2

[0066] The method for extracting and purifying black soybean glycoprotein of the present invention is specifically carried out according to the following steps:

[0067] S1, take black soybeans, peel them, and crush them into powder, the particle size of which is 0.1-1mm;

[0068] S2, take 1000g of black soybean powder, add absolute ethanol according to the solid-to-liquid ratio of 1:7g / mL, place in a Soxhlet extractor, heat at 70°C for 1.5h, and perform reflux degreasing treatment, and repeat the degreasing treatment 2-3 times;

[0069] S3, extract the degreased filter residue with 10 times the volume of distilled water at 70° C. for 3 hours, and suction filter;

[0070] S4, obtain crude sugar protein:

[0071] Concentrate the filtrate obtained in step S3 by 15 times with a rotary evaporator, add 5 times the volume of absolute ethanol at 0°C, completely precipitate for 30 hours, filter the precipitate with suction, collect the filter residue, dissolve it in distilled water, ...

Embodiment 3

[0075] The method for extracting and purifying black soybean glycoprotein of the present invention is specifically carried out according to the following steps:

[0076] S1, take black soybeans, peel them, and crush them into powder, the particle size of which is 0.1-1mm;

[0077] S2, take 1000g of black soybean powder, add absolute ethanol according to the solid-to-liquid ratio of 1:6g / mL, place it in a Soxhlet extractor, heat at 75°C for 1.8h, and perform reflux degreasing treatment, and repeat the degreasing treatment 2-3 times;

[0078] S3, extract the degreased filter residue with 12 times the volume of distilled water at 75°C for 2.5 hours, and suction filter;

[0079] S4, obtain crude sugar protein:

[0080] Concentrate the filtrate obtained in step S3 by 12 times with a rotary evaporator, add 4.5 times the volume of absolute ethanol at 2°C, completely precipitate for 24 hours, filter the precipitate with suction, collect the filter residue, dissolve it in distilled wa...

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Abstract

The invention discloses a method for extracting and purifying black soybean glycoprotein, which specifically comprises: taking black soybeans, peeling them, and pulverizing them into powder; taking 1000g of black soybean powder, adding absolute ethanol according to the solid-liquid ratio of 1:5-1:7g / mL , placed in a Soxhlet extractor, heated at 70-80°C for 1.5-2h, and subjected to reflux degreasing treatment; the degreased filter residue was extracted with 8-10 times the volume of distilled water at 70-80°C for 2-3h, and suction filtered The filtrate was concentrated and ethanol precipitated to obtain crude glycoprotein; DEAE-52 cellulose ion column chromatography was initially separated to obtain the eluent; the eluent of each group was separated and purified by Sephadex G-100 to obtain Dry powder of five pure black soybean glycoproteins. The invention obtains five kinds of pure black soybean glycoproteins, retains their biological activity, has high purity and high yield; fully utilizes raw materials, increases the yield and types of glycoproteins, and is convenient for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide modification, and relates to a method for extracting and purifying black soybean glycoprotein. Background technique [0002] Polysaccharides, also known as polysaccharides, are very complex biomacromolecular substances that are polymerized by multiple monosaccharides through two different glycosidic bonds. It has a wide range of sources, and its relative molecular mass is generally tens of thousands or even millions. It has rich and good biological activities, and can combine with proteins on the cell surface to form glycoproteins, making the cell membrane have a specific recognition function. In recent years, as people's research on polysaccharides has become more and more in-depth, many plant polysaccharides have been found to have special biological activities, and many traditional Chinese medicine polysaccharide products have begun to be put into clinical use. Studies have shown that p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C07K1/14C07K1/36C07K1/30C07K1/16
CPCC07K14/415
Inventor 张武霞赵晋忠李鹏岳爱琴杜维俊张永坡高春艳郭冬冬殷丛丛
Owner SHANXI AGRI UNIV
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