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Spinach cultivation medium and cultivation method

A technology of spinach and fermentation bacteria, which is applied in cultivation, soilless cultivation, plant cultivation, etc. It can solve the problems of imbalanced proportion, difficulty in meeting the needs of plant nutrients, and excessive nitrate content of spinach, so as to promote germination, increase germination rate, The effect of increasing affinity

Active Publication Date: 2017-11-24
QINGDAO ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, due to the excessive application of nitrogen fertilizers in agricultural production, the ratio of N, P, and K in the soil has been out of balance, and a large amount of nitrogen fertilizers has caused the nitrate content in spinach to seriously exceed the standard
Although organic fertilizers can be applied to farmland instead of inorganic compound fertilizers, organic fertilizers are slow-release fertilizers, and it is difficult to meet the needs of nutrients in the early growth process of plants. At present, there is an urgent need for an effective cultivation method that can achieve high yield and growth. Spinach with a short cycle, the obtained spinach can contain less nitrate, nitrite and soluble oxalic acid and other substances that are not easy for human health

Method used

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  • Spinach cultivation medium and cultivation method
  • Spinach cultivation medium and cultivation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for accelerating germination of spinach, the steps are as follows:

[0039] a. Soak the spinach seeds in 10% trisodium phosphate solution for 15-20 minutes, wash with clean water 3 times, 2 minutes each time;

[0040] b. Mix 50% formic acid aqueous solution, 1.5% ethanol solution of dibutyl phthalate and 2% ethanol solution of fatty acid polyoxyethylene ester in a volume ratio of 9:1:10 to prepare a germination solution, wash with water Soak the spinach seeds in the germination solution for 15-20 minutes;

[0041] c. Take out the spinach seeds in the germination solution, wash them once with clean water, and let them dry in the shade at room temperature for 15 minutes; then they can be sown on the cultivation medium or soil.

[0042] 1. Germination test of spinach seeds

[0043] 1.1 Experimental design

[0044] Choose 500 spinach seeds (purchased from Beijing Shengaofeng Seed Co., Ltd.) of Royal Green Giant F1 spinach seeds (purchased from Beijing Shengaofen...

Embodiment 2

[0057] Bacteria solution preparation:

[0058] Inoculate the Lactobacillus acidophilus strain into the activated medium for cultivation at a temperature of 37°C for 24 hours; inoculate the activated strain into a fermenter, control the temperature at 37°C to 43°C for 24h to 36h, and prepare fermentation broth.

[0059] The activation medium is: MRS medium, the composition is (g / L): peptone 10.0, meat extract 10.0, yeast extract powder 5.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0 , magnesium sulfate 0.58, manganese sulfate 0.28, cysteine ​​0.5, Tween 801.0, adjust pH 6.2-6.4.

[0060] The medium used for fermentation is: MRS medium, the composition of which is the same as above.

[0061] After the fermentation is completed, the content of Lactobacillus acidophilus in the bacterial liquid is ≥10 7 CFU / mL.

[0062] The Lactobacillus acidophilus (Lactobacillus acidophilus) is Lactobacillus acidophilus AS 1.2686, pur...

Embodiment 3

[0073] A kind of cultivation method of spinach, the steps are as follows:

[0074] 1. Preparation of fermented bacterial fertilizer:

[0075] 1) Take wheat bran and steam sterilize it at 100°C for 30 minutes, cool it to 30°C-37°C, and mix it with peat soil at a weight ratio of 6:1;

[0076] 2) Activate the Bacillus subtilis strain and expand the culture to prepare a concentration of ≥10 8 CFU / mL fermentation liquid, spray the fermentation liquid of Bacillus subtilis into the above materials, the inoculum amount is 8%, control the temperature at 30°C to 35°C, keep it moist, turn it once an hour, and ferment aerobically for 24 hours;

[0077] 3) To the material after aerobic fermentation, add wheat bran and distiller's grains with 1 / 5 of the total amount of peat soil and mix evenly, activate the Lactobacillus acidophilus strain and expand the culture to prepare a concentration ≥ 10 7 CFU / mL fermentation broth, spray the fermentation broth of Lactobacillus acidophilus into the ...

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Abstract

The invention discloses a spinach cultivation medium and a cultivation method, and belongs to the technical field of the crop growing. The spinach cultivation medium is formed by mixing fermentation bacterial manure, perlite and an inorganic fertilizer according to the weight ratio of 24:11:4. The fermentation bacterial manure is formed by the following steps: mixing wheat bran and turfy soil according to the weight ratio of 6:1, executing the aerobic fermentation by bacillus subtilis, and adding vinasse which is 1 / 5 of the total quantity of the wheat bran and turfy soil, uniformly mixing, and inoculating lactobacillus acidophilus for anaerobic fermentation; and adding rhodopseudomonas palustris solution to a fermented product. The inorganic fertilizer is formed by mixing urea, monopotassium phosphate and potassium nitrate according to the weight ratio of 2.5:1:4.5. The cultivation medium is capable of satisfying the nutrient requirements of the spinach in the different growing periods, preventing the plant diseases and insect pests, improving the yield, shortening the growth cycle, improving the content of VC and polyphenol, and reducing the content of nitrate, nitrite and a soluble oxalic acid.

Description

technical field [0001] The invention belongs to the technical field of crop cultivation, and in particular relates to a spinach cultivation substrate and a cultivation method. Background technique [0002] Spinach (Spinacia oleracea L.) belongs to the genus Spinach of Chenopodiaceae. It is widely planted in spring, autumn and winter in urban and rural areas in my country. It is an important green leafy vegetable and export vegetable in my country. Spinach has high nutritional value and is rich in vitamin B 1 , Vitamin B 2 , vitamin C, carotene, protein, folic acid, and minerals such as iron, calcium, phosphorus, etc., are known as "the king of vegetables" by Arabs. However, as a leafy vegetable, spinach is very easy to accumulate nitrate and oxalic acid, and nitrate intake can make people suffer from methemoglobinemia. At the same time, nitrate is the precursor of carcinogen nitrosamines, which can easily induce cancer in the human digestive system. Oxalic acid is an ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00A01C1/00C05G1/00
CPCA01C1/00A01G22/00C05B7/00C05F5/002C05F5/008C05F11/02C05F11/08C05F17/50C05C5/02C05C9/00C05D9/00
Inventor 李磊万述伟连之新王英磊崔潇王广华王珍青
Owner QINGDAO ACAD OF AGRI SCI
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