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Bovine parainfluenza virus pbiv3-b strain and its application

A PBIV3-B, bovine parainfluenza virus technology, applied in the direction of virus, antiviral agent, virus/bacteriophage, etc., can solve the problem that there is no bovine parainfluenza virus vaccine

Active Publication Date: 2020-02-07
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no commercial bovine parainfluenza virus vaccine and detection reagents in China

Method used

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  • Bovine parainfluenza virus pbiv3-b strain and its application
  • Bovine parainfluenza virus pbiv3-b strain and its application
  • Bovine parainfluenza virus pbiv3-b strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and culture identification of bovine parainfluenza virus PBIV3-B strain

[0024] 1 Source and standard of poisonous species

[0025] According to the application requirements of new biological products, combined with a large number of test data obtained by the present invention, with reference to the "Chinese Veterinary Pharmacopoeia" (2005 edition), the poisonous seeds for seedling production have been identified. illustrate.

[0026] 1.1 Sources of poisonous seeds

[0027] The virus species used for the manufacture of this product is the bovine parainfluenza virus PBIV3-B strain, which the inventor obtained after self-isolation and domestication from the diseased cattle in 2015. Because this strain is derived from the occurrence of typical bovine parainfluenza virus type 3 infection of cattle Compared with other isolated strains, it has a high proliferation titer on cells and good immunogenicity to cattle, so it is selected for the production of ...

Embodiment 2

[0056] The preparation of embodiment 2 inactivated vaccine

[0057] The preparation process of bovine parainfluenza virus PBIV3-B strain is prepared according to the currently mature commercial vaccine preparation technology. Judging from the quality inspection results of 3 batches of vaccines prepared in the laboratory and 5 batches of vaccines produced in the intermediate trial, the quality of the vaccine products prepared by this process is stable, and the efficacy inspection results fully meet the established quality standards.

[0058] 1 Preparation of virus solution

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Abstract

The present invention discloses bovine parainfluenza virus PBIV3-B strain and application thereof, the bovine parainfluenza virus PBIV3-B strain has the advantages of good immunogenicity and culture characteristics and the like, the bovine parainfluenza virus PBIV3-B strain is inoculated into MDBK cell to obtain a culture matter, after the culture matter is inactivated with (beta-Propiolactone), the culture matter is mixed with commercial MontanideTMISA15AVG adjuvant of SEPPIC of France for emulsion to obtain a bovine parainfluenza virus inactivated vaccine. Results showed that when the bovine parainfluenza virus inactivated vaccine prepared by the method is used for immunization of a weaned healthy negative cow, diseases caused by the bovine parainfluenza virus type 3 can be prevented, and the bovine parainfluenza virus inactivated vaccine has the advantages of safety, fast antibody production and lasting immunity period and the like.

Description

technical field [0001] The invention belongs to the field of biology, more specifically, to the field of biological vaccines, relates to a bovine infectious rhinotracheitis virus and applications thereof, and particularly relates to a self-isolated bovine parainfluenza virus PBIV3-B strain and the virus strain The prepared inactivated vaccine. Background technique [0002] Bovine parainfluenza is an acute, contact infectious disease caused by bovine parainfluenza virus 3 (PBIV3), also known as "transport fever". PBIV3 belongs to the family Paramyxoviridae and belongs to the genus Paramyxovirus. It is a single-stranded negative-strand enveloped RNA virus that mainly causes acute respiratory diseases in cattle and other ruminants. In 1959, Reisinger et al. first isolated PBIV3 from calf kidneys in the United States. After that, France, the former Soviet Union, Japan, Denmark, Canada, Australia, Panama and Italy and other countries have also isolated the virus one after anoth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/155A61P31/14C12R1/93
CPCA61K39/12A61K2039/5252C12N7/00C12N2760/18621C12N2760/18634
Inventor 崔尚金赵占中梁琳
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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