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A joint repair composition based on active ingredients of sturgeon cartilage and its preparation method

A technology of active ingredients and compositions, applied in the field of joint repair compositions based on active ingredients of sturgeon cartilage and its preparation, can solve the problems of poor soft tissue reconstruction and recovery, poor bone and joint repair ability, etc., and achieve prevention and Effects of treating joint degeneration, reducing pain, and inhibiting microvascular-derived factors

Active Publication Date: 2020-12-11
健乐高(福建)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This health food can better promote bone absorption, increase bone density, promote fracture healing, and delay osteoporosis, but its ability to repair bones and joints is poor, and the effect on soft tissue reconstruction and recovery is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A joint repair composition based on the active ingredient of sturgeon cartilage, and its parts by weight are: 80-95 parts of sturgeon cartilage type II collagen, 1-10 parts of sturgeon cartilage chondroitin sulfate, 5-15 parts of glucosamine, Calcium casein 0.1~1 part.

[0022] Glucosamine is glucosamine sulfate or glucosamine hydrochloride or N-acetylglucosamine.

[0023] A preparation method of a joint repair composition based on active ingredients of sturgeon cartilage is as follows:

[0024] 1) Degreasing: crush the cartilage tissue of sturgeon in a masher, add NaOH solution (0.08~0.14mol / L) according to the ratio of material to liquid 1:10~25 (w / v), stir at 2~5°C for 18~36 h (NaOH solution is replaced every 4-8 h), centrifuge at 15 000-20000 rpm for 20-30 min, collect the precipitate and wash until neutral, add n-butyl Soak in alcohol solution (15%) for 36-54 hours to remove residual fat;

[0025] 2) Decalcification: Add EDTA-2Na solution (0.42-0.49 mol / L, pH 7....

Embodiment 2

[0032] 1) Preparation of type II collagen from sturgeon cartilage:

[0033] ①Crush cartilage tissue of sturgeon in a masher, add NaOH solution (0.1 mol / L) according to the ratio of material to liquid 1:15 (w / v), stir at 4 °C for 30 h (NaOH solution is replaced every 5 h), 18 Centrifuge at 000rpm for 25 min, collect the precipitate and wash until neutral, add n-butanol solution (15%) to soak for 48 h according to the ratio of material to liquid 1:20 (w / v) to remove residual fat, 1:15 (w / v ) Add EDTA-2Na solution (pH 7.4, 0.5 mol / L) to the solid matter and soak for 18 h (the EDTA-2Na solution is replaced every 3 h) to decalcify; finally, the cartilage solid matter is washed to neutral and dry by distillation.

[0034] ②Put the above-mentioned pretreated sturgeon cartilage in the acetic acid solution (0.5 mol / L) according to the material-to-liquid ratio of 1:15 (w / v), stir and extract for 30 h, centrifuge at 18 000 rpm for 20 min, and collect the sediment; Add acetic acid soluti...

Embodiment 3

[0043] 1) Preparation of type II collagen from sturgeon cartilage:

[0044] ①Crush cartilage tissue of sturgeon in a masher, add NaOH solution (0.1 mol / L) according to the ratio of material to liquid 1:20 (w / v), stir at 3°C ​​for 30 h (NaOH solution is replaced every 5 h), 18 Centrifuge at 000rpm for 25 min, collect the precipitate and wash until neutral, add n-butanol solution (15%) to soak for 48 h according to the ratio of material to liquid 1:20 (w / v) to remove residual fat, 1:15 (w / v ) Add EDTA-2Na solution (pH 7.4, 0.5 mol / L) to the solid matter and soak for 18 h (the EDTA-2Na solution is replaced every 3 h) to decalcify; finally, the cartilage solid matter is washed to neutral and dry by distillation.

[0045] ②Put the above-mentioned pretreated sturgeon cartilage in the acetic acid solution (0.5 mol / L) according to the material-to-liquid ratio of 1:20 (w / v), stir and extract for 30 h, centrifuge at 18 000 rpm for 20 min, and collect the sediment; Add acetic acid solut...

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Abstract

The invention discloses a joint repairing composition based on sturgeon cartilage active components and a preparation method of the joint repairing composition. The joint repairing composition is prepared from the following components in parts by weight: 80 to 95 parts of sturgeon cartilage type-II collagen, 1 to 10 parts of sturgeon cartilage chondroitin sulfate, 5 to 15 parts of glucosamine and 0.1 to 1 part of calcium caseinate. The joint repairing composition has the beneficial effects that the prepared joint repairing composition based on the sturgeon cartilage active components has a relatively good joint repairing effect, can be used as medical food for patients suffering from joint swelling pain, osteoporosis and the like and also can be used as a daily health product for old people with weak joint functions.

Description

technical field [0001] The invention relates to the technical field of health care products and medicines, in particular to a joint repair composition based on active ingredients of sturgeon cartilage and a preparation method thereof. Background technique [0002] Sturgeon (Sturgeon) refers to fish of the order Sturgeon, which has high economic value. At present, there are 27 species of sturgeon in the world, and 8 species in my country. In recent decades, due to human factors such as environmental pollution and overfishing, some sturgeon species are on the verge of extinction. In order to save these endangered species, countries around the world have done a lot of work in the reproduction, release and breeding of sturgeon. So far, my country has introduced more than a dozen species of sturgeon, such as Russian sturgeon, European sturgeon, small sturgeon, and spoon-nose sturgeon, from abroad for research or artificial breeding. The main products of sturgeon farming in Chi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K38/01A61P19/02A61P29/00A23L33/17A23L33/125A61K31/737A61K31/7008A61K33/06A61K35/60
CPCA23V2002/00A61K31/7008A61K31/737A61K33/06A61K35/60A61K38/014A61K38/1709A23L33/125A23L33/17A61K2300/00A23V2200/30A23V2250/5422A23V2250/542A23V2250/50A23V2250/61
Inventor 蓝晨王斌杨帆
Owner 健乐高(福建)生物科技有限公司
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