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Traditional Chinese medicine volatile oil composition having effects of sterilization and disinfection, and air sterilization disinfection agent

A sterilization, air sterilization technology, applied in the directions of disinfectants, biocides, animal repellents, etc., can solve the problem of the scarcity of air sterilization and disinfectants, and achieve good antibacterial and anti-influenza virus effects and remarkable effects.

Active Publication Date: 2017-07-14
GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problem of the scarcity of air sterilizing and disinfecting agents from natural active sources in the prior art, the present invention provides a traditional Chinese medicine volatile oil composition with bactericidal and disinfecting effects and an air sterilizing and disinfecting agent containing the composition

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 raw material volatile oil

[0031] 1. Preparation of the volatile oil of Atractylodes atractylodes

[0032] (1) Pulverize the traditional Chinese medicine Atractylodes atractylodes, and then use a supercritical carbon dioxide extraction method to extract a supercritical carbon dioxide extract of Atractylodes atractylodes; the specific conditions of the supercritical carbon dioxide extraction method are: the extraction pressure is 50 MPa, and the extraction temperature is 55 °C, the flow rate is 35 liters / hour, the extraction time is 2 hours, and ethanol is added as the entrainer at the same time;

[0033] (2) Put the supercritical carbon dioxide extract of Atractylodes atractylodes obtained in step (1) on a silica gel column, and elute with a mixed solvent system composed of petroleum ether and acetone with a volume ratio of 95:5; collect the eluate, concentrate and dry it to obtain The volatile oil of Mao Atractylodis.

[0034] 2. Prep...

Embodiment 2

[0040] Embodiment 2 Antibacterial experiment of volatile oil of the present invention

[0041] Samples to be tested: Atractylodes argyi volatile oil, Atractylodes argyi volatile oil, Atractylodes argyi volatile oil, Atractylodes argyi volatile oil: Atractylodes argyi volatile oil=2:1, Atractylodes argyi volatile oil: Atractylodes argyi volatile oil=1:1, Atractylodes argyi volatile oil: Atractylodes mugwort volatile oil=1:3, Atractylodes argyi Volatile oil: Artemisia argyi volatile oil = 2:1, norfloxacin lactate. The above ratios are mass ratios.

[0042] Add 0.2 mL to the culture medium under sterile conditions at a concentration of 1×10 7 Bacteria solution per mL (see Table 1 for specific strains), smear evenly with a sterilized L-shaped smear stick, dry at room temperature for 5 minutes, put the Oxford cup into a petri dish with sterile tweezers, and then place it in the Oxford cup Add 200μL of the sample solution to be tested with a concentration of 1g / mL

[0043] , put ...

Embodiment 3

[0048] Embodiment 3 Anti-influenza A virus activity test of volatile oil of the present invention

[0049] Samples to be tested: Atractylodes argyi volatile oil, Atractylodes argyi volatile oil, Atractylodes argyi volatile oil, Atractylodes argyi volatile oil: Atractylodes argyi volatile oil=2:1, Atractylodes argyi volatile oil: Atractylodes argyi volatile oil=1:1, Atractylodes argyi volatile oil: Atractylodes mugwort volatile oil=1:3, Atractylodes argyi Volatile oil: Artemisia argyi volatile oil = 2:1, ribavirin. The above ratios are mass ratios.

[0050] Take the 96-well cell culture plate where MDCK cells grow to a single layer, discard the supernatant, wash twice with PBS, add 100 µL of the drug solution and virus solution diluted twice with MEM to each well, and set up a cell control group, The virus control group was incubated in an incubator at 37°C, saturated humidity, and 5% CO2. After 72 h, 0.5 mM WST-1 solution was added to each well (the concentration of WST-1 wa...

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Abstract

The present invention relates to the technical field of chemical industry, and specifically discloses a traditional Chinese medicine volatile oil composition having effects of sterilization and disinfection, and an air sterilization disinfection agent, wherein the traditional Chinese medicine volatile oil composition contains atractylodes lancea volatile oil and folium artemisiae argyi volatile oil, a mass ratio of the atractylodes lancea volatile oil to the folium artemisiae argyi volatile oil is 1-3:1-3, and the air sterilization disinfection agent contains 0.1-20% by mass of the traditional Chinese medicine volatile oil composition. According to the present invention, the atractylodes lancea volatile oil and the folium artemisiae argyi volatile oil have good effects of sterilization and disinfection, and particularly the synergistic sterilization and disinfection effect can be produced after the atractylodes lancea volatile oil and the folium artemisiae argyi volatile oil are mixed; and the air sterilization disinfection agent prepared by using the natural components of the atractylodes lancea volatile oil and the folium artemisiae argyi volatile oil as the raw materials has the significant effect and cannot cause harm to human body.

Description

technical field [0001] The invention relates to the technical field of chemical industry, in particular to a traditional Chinese medicine volatile oil composition with bactericidal and disinfecting effects and an air sterilizing and disinfecting agent. Background technique [0002] At present, the commonly used air disinfection methods in China include chemical reagent methods, such as peracetic acid, hydrogen peroxide, formaldehyde, 84 disinfectant, glutaraldehyde, etc., as well as physical methods such as ultraviolet irradiation and ozone generator. Although these methods can kill pathogenic microorganisms, have rapid action and low effective concentration, they each have certain defects, such as toxicity, irritation, metal corrosion, and poor lasting effect; many of them constitute pollutants themselves and affect the human body to varying degrees. Healthy, not suitable for a human environment; all personnel must leave the site during disinfection, and protective measures...

Claims

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Application Information

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IPC IPC(8): A01N65/12A01P1/00
Inventor 林琳曾罗麟范龙陈远彬吴蕾于旭华
Owner GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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