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Vaccine composition for preventing porcine reproductive and respiratory syndrome and preparation method and application thereof

A technology for respiratory syndrome and vaccine composition, which is applied in the field of vaccine composition for preventing porcine reproductive and respiratory syndrome and its preparation field, can solve the problems of complex disease, unsatisfactory vaccine preventive effect, etc., and achieves good immunogenicity , the effect of improving productivity

Active Publication Date: 2017-07-07
PU LIKE BIO ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the clinical preventive effect of the existing vaccines is not ideal, and the mixed infection of new PRRSV epidemic strains and highly pathogenic PRRSV causes clinical complications. Therefore, it is necessary to develop vaccines for the latest clinical conditions in my country. Vaccine composition to effectively prevent the epidemic of new porcine reproductive and respiratory syndrome in my country

Method used

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  • Vaccine composition for preventing porcine reproductive and respiratory syndrome and preparation method and application thereof
  • Vaccine composition for preventing porcine reproductive and respiratory syndrome and preparation method and application thereof
  • Vaccine composition for preventing porcine reproductive and respiratory syndrome and preparation method and application thereof

Examples

Experimental program
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Embodiment approach

[0019] As an embodiment of the present invention, the antigen of porcine reproductive and respiratory syndrome virus HNjz15 strain includes inactivated antigen, attenuated antigen, subunit antigen or synthetic antigen of porcine reproductive and respiratory syndrome virus HNjz15 strain or its culture peptide antigen. "Culture" is the subculture of different generations of the virus, and those skilled in the art know that only slight variations may occur in the gene sequence between different generations.

[0020] "Vaccine composition" means a pharmaceutical composition containing porcine reproductive and respiratory syndrome virus immunogenicity. The pharmaceutical composition can induce, stimulate or enhance pig's immune response against porcine reproductive and respiratory syndrome virus. The vaccine composition includes an immunizing amount of an inactivated vaccine of a strain of porcine reproductive and respiratory syndrome virus.

[0021] "Inactivated antigen", also ca...

Embodiment 1

[0055] Example 1 Collection and isolation of virus

[0056] Isolate from samples suspected of porcine reproductive and respiratory syndrome virus infection from Henan, collect porcine tonsil lymphoid tissue aseptically, add DMEM culture medium at 1:10 (volume ratio), grind, prepare tissue suspension, repeat 3 times After freezing and thawing, centrifuge at 12000r / min for 15min, collect the supernatant, filter it through a 0.22μm membrane filter, culture it on PAM cells at 37℃ for 1h, replace it with DMEM culture medium containing 2% calf serum, and culture at 37℃ 5th. Harvest the poisonous culture medium, freeze and thaw twice, collect the poison, and add DMEM culture medium containing 2% calf serum. The porcine reproductive and respiratory syndrome virus PCR detection kit (Beijing Century Yuanheng Animal Epidemic Prevention Technology Co., Ltd.) was used to detect the porcine reproductive and respiratory syndrome virus, and the result was positive; Detection, respectively d...

Embodiment 2

[0058] Example 2 Genetic Characteristics of Isolated Viruses

[0059] The genetic characteristics of the virus isolated in Example 1 were determined by genetic analysis. The genome of porcine reproductive and respiratory syndrome virus isolated on PAM cells was used as a template, reverse-transcribed into cDNA, and PCR was performed using the primers shown in Table 1. Primer Premier 5.0 was used to design primer sequences for amplifying GP5, GP2, and Nsp2 genes, respectively.

[0060] The PCR amplification system is as follows: Template cDNA 1μl, PrimerSTAR HS DNA Polymerase (2.5U / μl) 0.5μl, 5×PrimeSTAR TM Buffer10μl, upstream and downstream primers 1μl (10pmol / μl), dNTP Mix (2.5mM each) 4μl, and ddH 2 O Make up the volume to 50 μl. A two-step PCR reaction was performed: 2 min at 98°C; 10 s at 98°C, 1 min at 55°C, 1 mim / kb at 72°C, 30 cycles; 5 min at 72°C. The resulting PCR products were analyzed by electrophoresis on a 1% agarose gel containing ethidium bromide. PCR prod...

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Abstract

The invention relates to a vaccine composition for preventing a porcine reproductive and respiratory syndrome. The vaccine composition comprises an immunizing dose of antigen of a porcine reproductive and respiratory syndrome virus strain HNjz15, high-pathogenicity porcine reproductive and respiratory syndrome virus antigen and a pharmaceutically acceptable carrier. The vaccine composition can effectively resist a new epidemic strain and the attack of high-pathogenicity porcine reproductive and respiratory syndrome viruses, produces the synergistic effect on the protection effect of the high-pathogenicity porcine reproductive and respiratory syndrome virus antigen and improves the immune resistance ability of the antigen.

Description

technical field [0001] The invention belongs to the field of veterinary vaccines, and in particular relates to a vaccine composition for preventing porcine reproductive and respiratory syndrome, a preparation method and application thereof. Background technique [0002] Porcine reproductive and respiratory syndrome is caused by porcine reproductive and respiratory syndrome virus (porcinereproductive and respiratory syndrome virus, PRRSV), which is characterized by fever, abortion in sows, increased mortality of piglets before and after weaning, respiratory disorders in pigs of different ages, etc. disease with clinical features. The disease was first discovered in North Carolina in the United States (1987), and then successively found in Canada (1998), Germany (1990), and the United Kingdom (1991). In 1992, the International Veterinary Office (OIE) named the disease Porcine Reproductive and Respiratory Syndrome (PRRS). my country also reported the prevalence of this diseas...

Claims

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Application Information

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IPC IPC(8): A61K39/12A61K39/295A61P31/14
CPCA61K39/12A61K2039/5252A61K2039/70C12N2770/10034
Inventor 田克恭燕贺孙进忠张许科
Owner PU LIKE BIO ENG
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