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Mycelia polysaccharide of Nicotiana fumigatus and its preparation method and application

A technology of mycelium and tobacco pipe fungus, applied in the biological field, can solve the problems of restricting the development and utilization of medicinal fungus tobacco pipe fungus, and the shortage of wild fungal resources

Inactive Publication Date: 2019-04-09
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, with the destruction of natural conditions, the resources of wild fungi have become more and more scarce, which limits the further development and utilization of medicinal fungi, including N.

Method used

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  • Mycelia polysaccharide of Nicotiana fumigatus and its preparation method and application
  • Mycelia polysaccharide of Nicotiana fumigatus and its preparation method and application
  • Mycelia polysaccharide of Nicotiana fumigatus and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Fermentation of the mycelium of the smoke pipe fungus

[0022] Seed liquid culture: 100mL of fresh seed culture medium in a 250mL shake flask, Bjerkanderafumosa (Bjerkanderafumosa) inoculation and activation of 3 plates of mother seed, each 0.5cm 2 . Place on a constant temperature shaker at 28°C and 150r / min to shake for 8 days.

[0023] The above 1L seed medium: 0.1g / L CaCl 2 ,1g / L KH 2 PO 4 ,1.5g / L MgSO 4 , 2g / L yeast extract, 3g / L peptone, 20g / L glucose.

[0024] Shake flask fermentation culture: 200mL of fresh fermentation medium was placed in a 500mL shake flask, cultured for 8 days, and the culture conditions were the same as the seed culture medium to obtain the mycelium of the smoke tube fungus.

[0025] The above 1L fermentation medium: 0.5g / L MgSO 4 ,0.05g / L FeSO 4 ,1g / L KH 2 PO 4 ,0.02g / LCuSO 4 ,0.4g / L K 2 HPO 4 ,0.01g / L ZnSO 4 ,0.01g / L CoCl 2 ,0.08g / L MnSO 4 , 3g / L peptone, 53g / L corn flour.

Embodiment 2

[0026] Example 2: Extraction, Separation and Purification of Polysaccharides from the Mycelium of Fumicotus

[0027] Extraction of deproteoglycan from the mycelium of Fusarium smoky tube mycelium: wash the mycelium with distilled water, heat at 100℃ for 2h, filter and concentrate, add 4 times 95% ethanol, stir well, stand at 4℃ overnight, and collect the precipitate by centrifugation , Washed 3 times with absolute ethanol and ether, and freeze-dried the precipitate to obtain the mycelial crude polysaccharide BFM. The crude polysaccharide was deproteinized by the enzyme-Sevag method. After the 5% polysaccharide solution was mixed with pronase (enzyme: protein=1:50), a small amount of xylene was added for preservative, and 1% NaCl was used as the activator. The temperature was kept at 37°C for 24 hours. Then it was shaken with Sevag reagent (chloroform: n-butanol=4:1), centrifuged, repeated several times until no precipitation was produced, precipitated with absolute ethanol and ly...

Embodiment 3

[0030] Example 3: Structural analysis of homogeneous polysaccharides from the mycelium of Fumigatus

[0031] Analysis of the monosaccharide composition of the homogenous polysaccharide of the mycelium of P. smokyii. By acidolysis, hydrolysis and derivatization of the homogenous polysaccharide of P. smokyii mycelium, DBFM3, the monosaccharide-PMP derivative is obtained, which is combined with Shimadzu HPLC system The DIKMAInertsil ODS-3 column (4.6mm×150mm) was used to determine the monosaccharide composition of DBFM3 at a flow rate of 1mL / min. image 3 It is a monosaccharide composition chromatogram, (a) is a standard sample, (b) is a homogeneous polysaccharide DBFM3; the test results show that DBFM3 contains mannose, galacturonic acid and galactose, and the molar ratio is 1:18.16:0.702.

[0032] Infrared spectroscopy analysis of the homogeneous polysaccharides of the mycelium of the smoky smoky tube fungus Take dried homogeneous polysaccharide DBFM3 2mg, compressed with KBr, and de...

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Abstract

The invention discloses bjerkandera fumosa mycelium polysaccharide and a preparation method and an application thereof. The method comprises the following steps: fermenting the material by a shake flask and collecting the bjerkandera fumosa mycelium, performing hot water extraction, ethanol deposition, freeze-drying and protein removal by an enzyme-Sevag method to obtain the mycelium de-protein polysaccharide DBFM, through a DEAE-32 cellulose anion-exchange column, separating and purifying the material to obtain the polysaccharide DBFM3 with uniform mycelium. The prepared DBFM and DBFM3 have antioxidation and immunoloregulation effects, and have application potential for preparation of an anti-oxidant and an immunoadjuvant.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and specifically relates to Fumaria smokyii mycelium polysaccharide, a preparation method thereof, and application as an antioxidant and an immunomodulator. Background technique [0002] The polysaccharide component is the main active ingredient known in edible and medicinal fungi. The edible and medicinal fungal polysaccharide is called "biological response modifier" in the world. As a biological non-specific immune enhancer, it has immune regulation and anti-oxidation. , Anti-tumor, anti-virus, hypoglycemic and hypoglycemic activity. [0003] Bjerkandera fumosa (Bjerkandera fumosa) is a medicinal fungus belonging to the genus Bjerkandera, Polyporaceae, and Fusarium. The fruiting body of the wild smoke pipe fungus is considered to have anti-tumor (especially anti-uterine cancer) effects in the northeast folks, but so far, there is no relevant research on the composition and function of the smoke p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K39/39A61P39/06A61P37/04
CPCA61K39/39C08B37/0003C08B37/006
Inventor 李凡刘迪高莹陈珊
Owner NORTHEAST NORMAL UNIVERSITY
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