Preparation method of nano-hydroxyapatite-doped porous bionic bone scaffold

A technology of nano-hydroxyapatite and biomimetic bone, which is applied in the field of preparation of porous biomimetic bone scaffolds, can solve the problems of adverse effects and refractory degradation, and achieve pore penetration, good biocompatibility, good bioactivity and osteoconduction Effect

Inactive Publication Date: 2017-06-09
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage is that it is difficult to degrade, and the residual solvent will have an adverse effect on the body

Method used

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  • Preparation method of nano-hydroxyapatite-doped porous bionic bone scaffold
  • Preparation method of nano-hydroxyapatite-doped porous bionic bone scaffold
  • Preparation method of nano-hydroxyapatite-doped porous bionic bone scaffold

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Preparation of porous hyaluronic acid (5mg / ml)-gelatin (5mg / ml) composite scaffold doped with nano-hydroxyapatite:

[0035] In a water bath at 60°C, dissolve hyaluronic acid and gelatin in triple-distilled water to obtain a 5mg / ml hyaluronic acid solution and a 5mg / ml gelatin mixture; stir to remove air bubbles, mix the above two solutions, and configure the volume ratio 1:9 and 9:1 hyaluronic acid-gelatin solutions;

[0036] After nano-hydroxyapatite is ultrasonically dispersed, it is added to the above solutions according to different mass ratios (one of the solutions does not add nano-hydroxyapatite), to form a hyaluronic acid-gelatin suspension without nano-hydroxyapatite, Hyaluronic acid-gelatin suspension containing 1 mg / ml nano-hydroxyapatite and 50 mg / ml nano-hydroxyapatite;

[0037] The above-mentioned hyaluronic acid-gelatin and hyaluronic acid-gelatin-nano hydroxyapatite mixtures were left to defoam, poured into the mold, pre-frozen at -80°C for more than 3 ...

Embodiment 2

[0040] Preparation of porous hyaluronic acid (15mg / ml)-gelatin (0.2g / ml) composite scaffold doped with nano-hydroxyapatite:

[0041] In a water bath at 60°C, dissolve hyaluronic acid and gelatin in triple-distilled water to obtain a 15 mg / ml hyaluronic acid solution and a 0.2 g / ml gelatin mixture; stir to remove air bubbles, mix the above two solutions, and prepare a volume Hyaluronic acid-gelatin solutions with a ratio of 1:9 and 9:1;

[0042] After nano-hydroxyapatite is ultrasonically dispersed, it is added to the above solutions according to different mass ratios (one of the solutions does not add nano-hydroxyapatite), to form a hyaluronic acid-gelatin suspension without nano-hydroxyapatite, Hyaluronic acid-gelatin suspension containing 1 mg / ml nano-hydroxyapatite and 50 mg / ml nano-hydroxyapatite;

[0043] The above-mentioned hyaluronic acid-gelatin and hyaluronic acid-gelatin-nano hydroxyapatite mixtures were left to defoam, poured into the mold, pre-frozen at -80°C for ...

Embodiment 3

[0047] Cell culture and analysis evaluation of porous biomimetic bone scaffold doped with nano-hydroxyapatite:

[0048] The composite scaffold designed and prepared by the laboratory was sterilized. The scaffold was soaked in 75% ethanol for 3 hours, irradiated with ultraviolet light for 1 hour, and soaked in PBS containing 10% double antibody for deep sterilization. After surface modification with type Ⅰ collagen, the above nine groups of composite scaffolds were inoculated with osteoblast precursor cells at a seeding density of 2×10 6 cells / mL, add culture medium after 6 hours of cell attachment, and after 7 days of cell culture, perform relevant staining for cell death, apoptosis, etc., detect cell proliferation and differentiation, and analyze with Image Pro software. Depend on Figure 5 It can be seen that the osteoblast precursor cells are distributed in the pores and surfaces of the scaffold in the state of clusters or single cells, and the cells and the scaffold, and ...

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Abstract

The invention provides a preparation method of a nano-hydroxyapatite-doped porous bionic bone scaffold. According to the preparation method, a hyaluronic acid-gelatin-nano-hydroxyapatite cross-linked composite porous scaffold with good mechanical strength is prepared from hyaluronic acid, gelatin and nano-hydroxyapatite by virtue of a freeze-drying method and a chemical cross-linking method. The porous composite scaffold prepared by virtue of the preparation method has uniform aperture and very good cell adhesion capacity and mechanical strength and further has osteoconductivity, hole walls are thin, and pore spaces are penetrated. The preparation method has the advantages that the composite scaffold can be used for relatively truly simulating a bone tissue microenvironment, and parameters of aperture, porosity, degradation rate, water absorption rate, mechanical strength and the like of the scaffold can be adjusted and controlled by adjusting the concentration, volume ratio and freeze drying conditions of each component; the adopted materials have good biocompatibility, and the prepared scaffold can meet an internal porous structure of bone tissues; and the sources of the material are sufficient, and the preparation process is simple.

Description

[0001] Technical field: [0002] The invention relates to the technical field of novel biomaterials and tissue engineering, in particular to a method for preparing a porous bionic bone scaffold doped with nano-hydroxyapatite. [0003] Background technique: [0004] The present invention is based on tissue engineering. Bone tissue engineering is one of the effective ways to repair bone defects clinically, and has attracted extensive attention from scholars at home and abroad in recent years. The three or four elements of bone tissue engineering mainly include scaffold materials, seed cells, the integration of cells and scaffold materials, and the integration of implants and in vivo microenvironment. The process refers to the expansion and culture of seed cells in vitro, and then The amplified cells are transplanted to a scaffold material with good biocompatibility and degradability to continue culturing, so that the cells adhere to the scaffold to form a cell-scaffold complex; ...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/24A61L27/12A61L27/56C08J9/36
CPCA61L27/20A61L27/12A61L27/24A61L27/56A61L2300/112A61L2300/232A61L2300/252A61L2430/02C08J9/36C08J2305/08C08J2389/00C08J2405/08C08J2489/00C08L5/08
Inventor 秦建华张晓庆
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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