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Trichoderma polysporum fungus strain HZ-31 and application thereof to biological weeding

A technology of Trichoderma polyspores and Trichoderma, applied in the direction of microorganism-based methods, chemicals for biological control, applications, etc., can solve the problems of increased pollution, reduced drug efficacy, and increased costs, and achieve less negative environmental effects , low residue environment, high safety effect

Inactive Publication Date: 2017-05-10
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, excessive reliance and long-term large-scale use of a single herbicide have led to the emergence and development of weed resistance, resulting in reduced efficacy, increased dosage, increased cost, and increased pollution, directly threatening the continued use of herbicides. Safety of use and agricultural production

Method used

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  • Trichoderma polysporum fungus strain HZ-31 and application thereof to biological weeding
  • Trichoderma polysporum fungus strain HZ-31 and application thereof to biological weeding
  • Trichoderma polysporum fungus strain HZ-31 and application thereof to biological weeding

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A Trichoderma polysporum strain, which is classified as Trichoderma polysporum HZ-31 (Trichoderma polysporum), was preserved on September 12, 2016 at No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, China. General Microbiology Center of Culture Collection Management Committee, the preservation number is CGMCC No.12867. After the strain was cultured on a PDA plate for 7 days, dense white conidia were produced on the surface, and the conidiophores were arranged in hemispherical or cushion-like blisters. Four whorled, right-angled, conidia are oblong or spherical, and smooth. Combined with morphological characteristics and molecular identification results, the strain HZ-31 was judged to be Trichoderma polysporum.

Embodiment 2

[0028] The preparation of embodiment 2 wettable powder

[0029] 1. Determination of optimal fermentation conditions for strain HZ-31

[0030]The carbon and nitrogen source single factor test was carried out with PDA medium as the basic medium, and the optimal carbon and nitrogen source of the strain HZ-31 was determined by the sporulation yield and OD absorbance value of the spore suspension; rapeseed cake, wheat straw bran, Sheep manure, wheat bran, perlite and vermiculite were used as substrates, cultured after inoculating the bacterial solution, and the solid-state fermentation substrate with high spore production was determined; rapeseed cake was used as the substrate, mixed nutrients were added to the triangular flask, and each treatment Add 20 mL of sterile water, connect spore suspensions with different ratios of matrix volume fractions, and determine the optimum inoculation amount of liquid strains by counting with a hemocytometer; screen out the water content of the m...

Embodiment 3

[0042] The influence of embodiment 3 strain HZ-31 wettable powder on the pathogenicity of weeds

[0043] Collect fresh weed leaves, place them in a petri dish lined with sterile filter paper, prepare HZ-31 wettable powder, and inoculate the amount of 100uL / leaf. Take the leaves only inoculated with water as the control, and repeat each treatment three times. Place them in a light incubator at 25°C for moisturizing culture, and observe the disease of the leaves.

[0044] Transplant wild oats, Polygonum sorrel, Thingrass, Chenopodium, Dendrobium japonica, and Liza with normal leaf color at the 4-5 leaf stage in the field into flowerpots (Φ=15cm), and cultivate them in the laboratory; Dissolve the wettable powder of HZ-31 and spray it on the transplanted healthy weed plants. The plants inoculated with clear water were used as the blank control, and three repetitions were set. After inoculation, the weed plants were placed at room temperature, and the degree of weed disease was ...

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Abstract

The application discloses a trichoderma polysporum fungus and application thereof to biologial weeding. A strain of the trichoderma polysporum fungus is named in a classified mode as trichoderma polysporum, is completely named as trichoderma polysporum HZ-31, is preserved in China General Microbiological Culture Collection Center on September 12, 2016, and has a preservation number of CGMCC No.12867. The invention provides wettable powder consisting of trichoderma polysporum fungal spore powder obtained by fermenting 10 to 15% of kaolin, 6% of polyvinyl alcohol, 3% of dextrin, 3% of nekal, 1% of bentonite and solid. According to the invention, aiming at common weeds in broad bean and pea fields, such as avena fatua, persicaria lapathifolia, lepyrodiclis holosteoides, chenopodium album, elsholtzia densa, and polygonum aviculare, the trichoderma polysporum fungus wettable powder is prepared, is used for biological weeding, can effectively and safely control, prevent and treat part of common farmland weeds, is low in cost, has no pollution, and is low in residue and environmental-friendly.

Description

technical field [0001] The application belongs to the field of microorganisms applied to agricultural plant protection, and in particular relates to a Trichoderma polyspora strain HZ-31 and its application in biological weeding. Background technique [0002] With the advancement of social civilization and the improvement of public health awareness, the negative effects of extensive, long-term and large-scale use of chemical herbicides are becoming more and more apparent, which has attracted global attention. On the one hand, the application of a large number of chemical herbicides has brought environmental pollution, especially the application of long-lasting herbicides has caused residual toxicity and phytotoxicity, resulting in reduced crop production and even human and animal poisoning. At present, more than 100 chemical herbicides have been banned or canceled in more than 30 countries around the world. On the other hand, excessive reliance and long-term large-scale use ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N25/14A01N63/04A01P13/00C12R1/885
CPCA01N25/14A01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 朱海霞马永强郭青云程亮魏有海郭良芝
Owner QINGHAI UNIVERSITY
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