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TiO2 mesoscopic crystal based trypsin photoelectrochemical detection method

A trypsin and photoelectrochemical technology, applied in the field of trypsin photoelectrochemical detection based on TiO2 mesocrystal, can solve the problems of low photoelectric conversion efficiency and large forbidden band width.

Inactive Publication Date: 2017-01-04
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, TiO 2 The band gap is large and can only be excited by ultraviolet light, so the photoelectric conversion efficiency is low in the visible light region

Method used

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  • TiO2 mesoscopic crystal based trypsin photoelectrochemical detection method
  • TiO2 mesoscopic crystal based trypsin photoelectrochemical detection method
  • TiO2 mesoscopic crystal based trypsin photoelectrochemical detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of TiO-based 2 The preparation method of the photoelectrochemical sensor of mesoscopic crystal (such as figure 1 shown):

[0041] (1) Pretreatment of the glassy carbon electrode: the glassy carbon electrode is first mechanically polished on the suede covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning until it is cleaned. Wash thoroughly with ethanol, dilute acid and water;

[0042] (2) Add dropwise 4μL of TiO with a concentration of 3mg / ml 2 Put the anatase mesogen suspension on the surface of a clean glassy carbon electrode, dry it under an infrared lamp, and cool it to room temperature;

[0043] (3) Immerse the electrode in 0.5 mg / ml polyacetimide solution for 40 min, and dry it at room temperature;

[0044] (4) Continue to drop 5 μL of glutaraldehyde solution with a concentration of 5.0 wt.% on the surface of the PEI / OAM modified electrode, place i...

Embodiment 2

[0047] Preparation of OAM materials:

[0048] The titanate nanowire precipitation was washed with dilute acetic acid solution, adjusted to pH 3.5, and dried at 60°C for 12 h to obtain the precursor titanate nanowires. Take 400mg of precursor titanate nanowires and disperse them in 70ml of acetic acid solution, react in a 100°C polytetrafluoroethylene autoclave at 200ºC for 48h, wash the obtained product by centrifugation with distilled water and absolute ethanol, and dry at 60ºC for 12 hours and calcined at 400ºC for 30 minutes to remove residual organic matter and obtain TiO2 Octahedral anatase mesoscopic crystals. Electron emission scanning electron microscope (SEM) image of OAM and nitrogen adsorption and desorption process diagram, such as Figure 2A and Figure 2A As shown in the inset, OAM is a symmetrical octahedral particle with a size of 40-100 nm. The nitrogen adsorption and desorption process has a type IV curve with an obvious hysteresis loop isotherm, which prov...

Embodiment 3

[0050] Preparation of B-CODs@petide solution:

[0051] (1) Preparation of B-CODs: 2.1 g of hydroquinone, 10 ml of acetone and 5.16 ml of BBr3 solution were mixed and transferred to a 25 mL polytetrafluoroethylene autoclave and heated at 200°C for 2 H. After the reaction was complete, the autoclave was cooled to room temperature, and the solution was concentrated by rotary evaporation to obtain B-CODs. Transmission electron microscopy (HRTEM) images of B-CODs, such as Figure 2C , indicating that B-CODs also have a lattice structure. The ultraviolet-visible (UV) spectrum and fluorescence (FL) spectrum of B-CODs, such as Figure 2D As shown, it is indicated that B-CODs have high light absorption, and photogenerated electrons and holes are easily recombined, which can effectively improve the sensitivity of PEC biosensors.

[0052] (2) Preparation of B-CODs@petide solution: 10 μL of boron-doped carbon quantum dots with a concentration of 10 mg / ml was dispersed in 50 μl of an AP...

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Abstract

The invention discloses a TiO2 mesoscopic crystal based trypsin photoelectrochemical detection method. According to the method, a polyethylenimine(PEI)-sensitized TiO2 octahedral anatase mesoscopic crystal is utilized as a photoactive substrate material, and a signal amplification function on the substrate material by boron-doped carbon quantum dot labeling peptide (B-CODs@petide) is utilized to prepare a photoelectrochemical sensor to be applied to trypsin detection. Based on the stability and excellent conductivity of PEI, PEI and OAM can be compounded to accelerate the transfer rate of photo-generated electrons and improve photocurrent signals; through the introductions of B-CODs with the competitive light capturing ability and space hampered peptide chains, the photocurrent signals can be significantly reduced; when a modified electrode is incubated in a trypsin solution, trypsin can conduct catalytic hydrolysis on peptide bonds, and B-CODs@petide can be released from the surface of the electrode, so as to improve the photocurrent signals; the photocurrent signals and the trypsin concentration are linear in the range of 1*10<-7> to 1.0 mg / mL. The method can be used for monitoring screening of various proteases and inhibitors in early diagnoses of different diseases.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and specifically relates to a TiO-based 2 Photoelectrochemical detection of trypsin in mesoscopic crystals. Background technique [0002] In recent years, the construction of efficient biosensors for simple protein detection in medical diagnosis and pathogen identification has attracted increasing attention. As a diagnostic tool, tumor markers play an important role in predicting and diagnosing cancer to enable early intervention and better management of the disease. Trypsin is an important digestive enzyme produced by the pancreas, which controls the exocrine function of the pancreas. In addition, a large number of diseases are closely related to changes in trypsin concentration levels, for example, cystic fibrosis, pancreatitis, gangrene, and meconium ileus. Therefore, the development of a simple and effective bioassay of trypsin and the monitoring...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/30G01N27/327
CPCG01N27/305G01N27/308G01N27/3278G01N27/416
Inventor 戴宏林燕语高利红
Owner FUJIAN NORMAL UNIV
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