A kind of method for preparing Polygonum cuspidatum resveratrol and emodin

Abstract

A method of preparing polygonum cuspidatum resveratrol and emodin belongs to the technical field of separation and preparation of the resveratrol and emodin. The method employs a polygonum cuspidatum resveratrol extract containing the emodin as a raw material, and includes the following steps: preparing a MCI separation filler equilibration column, preparing a polygonum cuspidatum resveratrol ethanol solution, preparing a resveratrol separation liquid, preparing concentrated liquids of the resveratrol and the emodin, preparing ethanol-removed concentrated liquids of the resveratrol and the emodin, and preparing freeze-dried powders of the resveratrol and the emodin, wherein the purity of the polygonum cuspidatum resveratrol is more than 99% and the purity of the emodin is more than 95%. Production devices in the method are all industrial devices. The method has mild production conditions and is suitable for large-scale production, and the product satisfies market demand. Through the membrane technology and a solvent recovery system, the method has low energy consumption and the material can be recycled, thereby significantly reducing production cost. The resveratrol and emodin products are widely used in the fields of medicines, health-caring foods, etc.

Description

1. Technical field [0001] The invention belongs to the technical field of separation and preparation of resveratrol and emodin, and in particular relates to a method for preparing polygonum cuspidatum resveratrol and emodin. 2. Background technology [0002] Resveratrol, referred to as stilbenol, is a non-flavonoid polyphenol compound containing stilbene structure, which is widely found in grapes, knotweed, peanuts and other plants. Biological and medical studies have shown that resveratrol has anti-oxidation, anti-aging, anti-tumor, anti-coagulation and coronary atherosclerosis, anti-inflammatory and anti-bacterial, prevention of high blood pressure and heart disease, improvement of blood circulation, lower blood sugar concentration, balance insulin Secretion, immune protection and other active effects, widely used in medicine, health food and cosmetics and other fields. Emodin is an anthraquinone compound, including macrocycline methyl ether, rhein, chrysophanol, emodin g...

AI Technical Summary

Problems solved by technology

[0004] Existing methods for separating resveratrol and emodin from Polygonum cuspidatum, such as "Separation and Purification of Resveratrol with High Concentration Agarose Gel Microspheres" published in the "Abstracts of the 9th Session of the 28th Annual Academic Conference of the Chinese Chemical Society" in 2012 "The paper introduces the method for separating resveratrol from Polygonum cuspidatum with agarose gel microspheres. The defects of this method are: ① using acetic acid and ethanol aqueous solution as the mobile phase to separate resveratrol in the crude extract of Polygonum cuspidatum. On the basis of resveratrol, further separation of resveratrol and acetic acid is required

However, the concentration of acetic acid and ethanol in the recovery solution concentrated under reduced pressure has changed, so it must be recalibrated before each reuse, and the operation is cumbersome.

Another example is the article "Study on the Separation of Resveratrol in Polygonum cuspidatum by Gel Column Chromatography" published in Volume 21 of "Natural Product Research and Development" in 2009, which introduces the separation of resveratrol from Polygonum cuspidatum with Sephadex LH-20 gel. Method, the shortcoming of this method is: 1. directly separate with Polygonum cuspidatum thick extraction concentrate, because there is a large amount of impurity in the thick extraction concentrate of Polygonum cuspidatum, and gel chromatography only relies on the molecular diameter difference of isolate to realize separation, and separation precision is limited, Therefore, the separation effect is not good, and the purity of the isolated resveratrol only reaches 86.16%.

② After gel separation, the separation liquid has not been further refined and has no industrial value