Application and preparation method of entagenic acid

A technology of salicylic acid and diabetes drug, applied in the field of medicine, can solve problems such as unequal toxic and side effects, and achieve the effects of small toxic and side effects, reliable action and small effective dose

Inactive Publication Date: 2016-10-12
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the drugs for treating diabetes mainly include biguanides, thiazolidinediones, sulfonylureas, α-glucosidase inhibitors, etc., but all of them have various toxic and side effects.

Method used

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  • Application and preparation method of entagenic acid
  • Application and preparation method of entagenic acid
  • Application and preparation method of entagenic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preparation of total saponins and chinensis acid

[0030] 1 Preparation of the total saponins of Tengzi: Take the seeds of Tengzi (5.3 kg), crush them, soak and extract three times with 6 times the volume percentage concentration of 70% ethanol aqueous solution, each time for 24 hours, combine the extracts and recover the ethanol to obtain the extract ( 1288g), the extract is dissolved on the HP-20 macroporous resin column with 60℃ hot water, the sample amount is based on the amount of medicinal materials and the resin weight ratio is (1:13), and the volume percentage concentration of 10 column volumes is 30% Wash and remove impurities with 5 column volumes of 50% ethanol aqueous solution, collect the eluate of 50% ethanol aqueous solution, concentrate, and dry to obtain the total saponins of Tengzi.

[0031] 2 Preparation of tantanic acid (EA): take the total saponins of tantanic acid, add 20 times the amount of 2 mol / L HCl, hydrolyze at 90°C for 3 hours, adjust ...

Embodiment 2

[0035] Example 2: Comparison of EA and similar compounds with similar structures on glucose consumption of rat skeletal muscle cells (L6).

[0036] 1Materials and methods

[0037] 1.1 Materials: oleanolic acid, ursolic acid (content ≥98%, China National Institute for the Control of Pharmaceutical and Biological Products), Asiatic Acid (content ≥98%, Shanghai Yuanye Biological Technology Co., Ltd.), echinocystic acid (content ≥98 %, Tianjin Yifang Technology Co., Ltd.); L6 cell line was purchased from Shanghai Cell Bank of Chinese Academy of Sciences; Trypsin, fetal bovine serum (Hyclon, USA), α-MEM medium (Gibco, USA), glucose oxidase assay kit ( Intech Innovation Technology Co., Ltd.), CO 2 Incubator (ThermoScientific Company, USA); XDS-1 inverted microscope (Chongqing Optical Instrument Factory); Microplate reader (Tecan infinite M2000, Switzerland); Ultra-clean workbench (Suzhou Purification Equipment Factory).

[0038] 1.2 Cell culture

[0039] L6 cells were grown in α-MEM mediu...

Embodiment 5

[0050] Example 5: Effect of EA on spontaneous type 2 diabetes model db / db mice

[0051] 1. Experimental materials

[0052] 1.1 Dosing sample: homemade EA sample (purity determined by HPLC method ≥96%), reference drug: metformin hydrochloride (Aladdin, batch number: M107827). All samples are prepared with 0.25% CMC-Na.

[0053] 1.2 Experimental animals: 40 7-week-old male BKS-db / db mice, 8 littermate heterozygous db / m mice, all purchased from Nanjing Institute of Model Animals (certificate number: SCXK (苏) 2010-0001 ). The db / db homozygous mice had blood glucose ≥15mmol / L two hours after meal, which was consistent with the symptoms of diabetes. They were all fed with ordinary feed, 12h light / dark, room temperature 22±1℃, humidity 50%-60%.

[0054] 1.3 Main reagents and equipment

[0055] Nuovo Rui; triglyceride kit (Nanjing Jiancheng Institute of Bioengineering, batch number: 20150127), total cholesterol test kit (Nanjing Jiancheng Institute of Bioengineering, lot number: 20150521), ...

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Abstract

The invention discloses application and a preparation method of entagenic acid. Entagenic acid can be applied to preparation of medicines for preventing and treating diabetes: entagenic acid, which is used as an active ingredient, and a pharmaceutically acceptable carrier or auxiliary material are prepared into a medicine or a medicine composition for preventing and treating diabetes. Compared with the conventional hypoglycemic medicines, entagenic acid (EA) has the advantages of being less in effective dose, reliable in function, less in toxic or side effect, and the like, and has the curative effects of not only reducing the blood glucose, but also playing an important role in prevention and treatment of diabetic complications, so as to achieve considerable development and application value in the aspect of resisting diabetes.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application of tannin acid derived from natural medicine and a preparation method thereof. Background technique [0002] Diabetes mellitus (DM) is a chronic metabolic disease characterized by high blood sugar caused by insufficient absolute or relative secretion of insulin. Globally, the incidence of DM is increasing year by year, and it has become the third major disease threatening human health after cancer and cardiovascular and cerebrovascular diseases. According to the World Health Organization report, the number of diabetes patients worldwide reached 387 million in 2014 and is expected to increase to 592 million by 2035, an increase of 53%. Type 2 diabetes (T2DM) patients account for more than 90% of DM patients, so the treatment of type 2 diabetes is the key to preventing and treating diabetes. At present, the drugs used to treat diabetes mainly include biguan...

Claims

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Application Information

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IPC IPC(8): A61K31/56C07J63/00A61P3/10A61P3/00A61P3/06
CPCA61K31/56C07J63/008
Inventor 熊慧梅之南赵平张胜男
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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