Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Avian nephropathogenic infectious bronchitis virus strain as well as vaccine composition, preparation method and application thereof

A vaccine composition and technology for bronchitis, applied in biochemical equipment and methods, microorganism-based methods, antiviral agents, etc., can solve the problems of inability to protect nephrotype epidemic strains, pass weak evaluation tests, and low homology

Active Publication Date: 2016-07-27
PU LIKE BIO ENG
View PDF8 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The patent application CN101514334A discloses that a virulent strain of infectious bronchitis virus tl / CH / LDT3 / 03 strain isolated is continuously passaged in 9-10 day-old SPF chicken embryos to weaken it, and it is passed to 120 generations to weaken it. The evaluation test is qualified. It is used as an attenuated vaccine strain and named LDT3-A. However, the S1 gene sequence of the vaccine strain has low homology to the current IBV epidemic strains in my country and cannot provide complete protection for the current kidney-type epidemic strains.
[0006] Research on Chicken Infectious Bronchitis (Nephrophilic Type) Live Vaccine (W93 Strain) Journal of the Chinese Academy of Sciences, 2001, 23 (6): 436-440) disclosed that a strain of attenuated strain W93 strain was obtained through chicken embryo continuous subculture attenuation, which was applied to the immune prevention of young chickens, but the isolation and subculture of this strain was earlier. , which is far away from the gene level of the current epidemic strains and cannot provide effective protection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Avian nephropathogenic infectious bronchitis virus strain as well as vaccine composition, preparation method and application thereof
  • Avian nephropathogenic infectious bronchitis virus strain as well as vaccine composition, preparation method and application thereof
  • Avian nephropathogenic infectious bronchitis virus strain as well as vaccine composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Isolation, Identification, Preservation and Gene Sequencing of Chicken Kidney Type Infectious Bronchitis Virus

[0062] 1.1 Isolation, Identification and Preservation of Chicken Kidney Type Infectious Bronchitis Virus

[0063] Chicken kidney-type infectious bronchitis virus was isolated in early 2013 by the technicians of Pulaike Bioengineering Co., Ltd. from the obviously diseased kidney tissue (swollen, mottled) of sick chickens suspected of outbreaks of infectious bronchitis Obtained, chicken embryos were inoculated and expanded to obtain virus liquid, and the obtained virus liquid could cause typical lesions of infectious bronchitis virus such as dehydration, curling up, and small development of inoculated chicken embryos; the obtained virus was confirmed by negative staining electron microscopy and RT-PCR detection. Avian infectious bronchitis virus. The animal regression experiment proved that the strain can cause the clinical symptoms of chicken kidney...

Embodiment 2

[0068] The preparation of embodiment 2 chicken kidney type infectious bronchitis inactivated vaccines

[0069] 2.1 Establishment of seed batches for the production of chicken infectious bronchitis virus PLK strain

[0070] Take the isolated PLK strain, dilute 100 times with sterilized saline, inoculate in the allantoic cavity of 9-10 day old SPF chicken embryos, inoculate 0.1ml per embryo, seal the pinhole after inoculation, and incubate at 37°C. Discard the 24h dead embryos, observe the chicken embryos until 48h, put the air chamber of the 30-72h chicken embryos upward, and cool them at 4°C for 4-24h. Harvest the chicken embryo allantoic fluid, mix several chicken embryo allantoic fluids into a group, put them in a sterilized bottle, store them at 2-8°C, and do a sterility test at the same time, and use SPF chicken embryos in this way to reduce the infectivity of chickens. The virulent strain of bronchitis virus PLK strain has been passed down continuously for 13 generations...

Embodiment 3

[0108] Embodiment 3 Preparation of attenuated strain of chicken kidney type infectious bronchitis virus strain

[0109] 3.1 Test process

[0110] 3.1.1 Virus passage

[0111] The chicken kidney-type infectious bronchitis virus PLK strain isolated in Example 1 was continuously passaged and attenuated, specifically: the PLK strain was continuously passaged in 9-10 day-old SPF chicken embryos, and the virus of each generation passed through the allantoic Inoculate 5 SPF chicken embryos in the cavity, dilute the virus solution of the previous generation 100 times each time, inoculate 100 μL per embryo, and incubate in a 37°C incubator for 48 hours (discard dead embryos after 24 hours). After 48 hours at 4°C, all the chicken embryos were taken out and placed at 4°C for 24 hours, the pathological changes of the chicken embryos were observed and recorded, and the allantoic fluid of the chicken embryos was aseptically collected for the next passage. Chicken embryos with typical lesi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of veterinary biological products, and relates to a strain of avian nephropathogenic infectious bronchitis virus containing a gene coding sequence SEQ ID No.2 which codes a protein and an attenuated virus strain. Serial passage of the virus strain is carried out to 110-140th generation in chicken embryos, and the attenuated virus strain is obtained. The invention also relates to a vaccine composition which is prepared by using the virus strain, the attenuated virus strain and antigen proteins thereof, as well as a preparation method and an application thereof. A vaccine composition containing inactivated totivirus antigen, live attenuated totivirus antigen and subunit antigen can be used for effectively preventing diseases related to avian nephropathogenic infectious bronchitis.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to chicken kidney type infectious bronchitis virus strain and vaccine composition, preparation method and application thereof. Background technique [0002] Chicken infectious bronchitis is an acute, highly contagious infectious disease caused by coronavirus. According to the tissue tropism of the virus and the lesion caused by the virus, it is often divided into respiratory type, renal type, reproductive type and glandular stomach type. [0003] Kidney infectious bronchitis was first reported in the United States in 1948. In 1962, Winterfied et al. discovered nephropathic infectious bronchitis virus in the United States, and named it Holte strain, Gray strain, CV56b strain, Wolgemuth strain, etc. (WinterfieldRW, HitchnerSB.Etiologyofaninfectiousnephritis-nephrosissyndromeofchickens. Res. 1962, 23:1273-1279). In the same year, Cumming isolated N1 / 62 (T str...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N7/08A61K39/215A61P31/14C07K14/165C12R1/93
Inventor 张许科孙进忠肖亚朋颜世君田克恭
Owner PU LIKE BIO ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com