Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Separating preparation method of micro droplets and microarrays

A separation method and micro-droplet technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the difficult separation of a large number of micro-droplets and the simple preparation of microarrays, difficult micro-droplets Separation, large volume of micro-droplets and other problems, to achieve the effect of low requirements, less samples and high separation efficiency

Active Publication Date: 2016-06-22
INST OF CHEM CHINESE ACAD OF SCI
View PDF10 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently used method has many defects, including the large amount of sample required, expensive or complicated equipment, the prepared micro-droplets are surrounded by the oil phase and it is difficult to separate, and the prepared micro-droplets have a large volume.
For very expensive or extremely small samples, it is difficult to achieve the separation of a large number of microdroplets and the easy preparation of microarrays using current technologies

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Separating preparation method of micro droplets and microarrays
  • Separating preparation method of micro droplets and microarrays

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A glycol droplet array was prepared using a drop of glycol solution. The silicon wafer is cleaned, and the specific steps are as follows: the silicon wafer is ultrasonicated three times with acetone and ethanol solutions respectively, and rinsed with deionized water. Then immerse the silicon wafer in a toluene solution with a volume fraction of 1% octadecyltrichlorosilane for 10 minutes, take it out and blow it dry with nitrogen. The whole process is carried out in a clean room to prevent silicon wafers from being polluted by dust in the air. At this time, the contact angle of the silicon wafer was 106°. The preparation of the hydrophilic regions then proceeds. Place the silicon wafer under a photomask, and then expose it to ultraviolet light with a wavelength of 365nm for 10 minutes. During the exposure process, octadecyltrichlorosilane molecules in the light-transmitting region decompose, and this region becomes hydrophilic at the same time. Use a pipette to fix a...

Embodiment 2

[0027] Prepare the dye pattern with a drop of the dye solution. The filter paper commonly used in the laboratory was modified by hydrophobization. The experimental method is as follows: put the filter paper in a clean vacuum desiccator, place a glass slide in it, and drop a drop of perfluorodecyltrimethoxysilane on the glass slide. Then use an oil pump to evacuate the vacuum desiccator for 10 minutes. Then place the desiccator in a 60°C oven for 2 hours. After removal, the static contact angle of the filter paper was 140°. The preparation method of the hydrophilic region of the hydrophobic filter paper is as follows: 5 milliliters of water and 5 milliliters of ethylene glycol are evenly mixed, and a mass fraction of 1% of PAA (molecular weight 3000) is added thereto. After stirring evenly, it can be used as ink for inkjet printing. Print on the hydrophobic filter paper with an inkjet printer, the area where the ink is printed becomes a hydrophilic area, and the area that i...

Embodiment 3

[0029] Preparation of sodium chloride crystals. Hydrophilic substrate preparation: The slides were sonicated three times with ethanol and acetone, rinsed with distilled water, and dried with nitrogen. The whole process is carried out in a clean room to ensure that the surface of the glass sheet is not polluted by impurities in the air. The surface is then patterned with hydrophobic Teflon by microfabrication. The pattern thickness is between 50 nanometers and 10 micrometers. At this time, the surface covered by Teflon is a hydrophobic region, and the region not covered by the Teflon surface is a hydrophilic region. A drop of saturated sodium chloride solution was fixed with a dropper, and the pressure of 200 μN and the speed of 1 mm / s were slid on the surface of Teflon / hydrophilic glass with hydrophilic and hydrophobic patterns. Teflon surface has little adhesion to saturated sodium chloride solution, and hydrophilic glass sheet has strong adhesion to saturated sodium chlor...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of material interfacial wetting, and discloses a method used for preparing micro droplets and microarrays via separating single droplets. According to the method, droplets are dropped onto a patterned hydrophilic / hydrophobic substrate, contact force of the droplets with the patterned hydrophilic / hydrophobic substrate is controlled, and relative slip velocity between the droplets and the patterned hydrophilic / hydrophobic substrate is controlled, so that size accurate control separation of the droplets can be realized so as to obtain the micro droplets and microarrays. The method can be used for preparing a large amount of micro droplets from one drop of a sample solution, the size of obtained micro droplets is small, requirement on the sample solution is low, less sample is needed, separation efficiency is high, equipment is simple, operation is convenient, preparation cost is low, and application range is wider. The method possesses a promising application prospect in the fields of surface patterning, biological analysis and detecting, chemical reaction, and drug development.

Description

technical field [0001] The invention belongs to the technical field of material interface infiltration, in particular to a method for separating a single droplet to prepare a microdroplet and a microarray. Background technique [0002] Micro-droplet preparation has broad application prospects in the fields of biological analysis and detection, chemical reaction, drug development, and surface patterning. At present, the micro-droplet preparation method mainly includes physical confinement (references: R.J.Jackman, D.C.Duffy, E.Ostuni, N.D.Willmore, G.M.Whitesides, Anal.Chem.1998,70,2280-2287; E.Ostuni, C.S.Chen, D.E.Ingber, G.M.Whitesides, Langmuir2001, 17, 2828-2834.), T-shaped channel microfluidic preparation (related patent numbers: CN104084247A, CN104107734A, CN104130932A), printing preparation (related patent numbers: CN104004652A, CN103974547A), etc. However, the currently used method has many defects, including the large amount of sample required, expensive or complic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
Inventor 李会增杨强李明珠宋延林
Owner INST OF CHEM CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com