Preparation method of DNA base pair cross-linked supermolecular hydrogel

Abstract

The invention provides a preparation method of DNA base pair cross-linked supermolecular hydrogel. Adenine and thymine are respectively modified to obtain a double bond connected base monomer, water serves as a solvent, the water and hydrophilic monomer acrylamide are subjected to thermal initiation by an initiator potassium persulfate, a high-molecular polymer with a three-dimensional network structure is formed through free radical copolymerization, and the polymer is subjected to the hydrogen-bond interaction of complementary pairing of DNA base pairs to obtain the DNA base pair cross-linked supermolecular hydrogel. According to the preparation method, the base pairs contained in a living body are introduced to polyacrylamide hydrogel, not only terminal connection is adopted, and the base pairs are inlayed into macromolecular chain segments, so that a large number of base pair hydrogen-bond interactions exist among chains, the mechanical properties are affected and stronger and more sensitive response is produced to salt and pH. The influence of the organic solvent is eliminated, and the DNA base pair cross-linked supermolecular hydrogel has good biocompatibility, viscosity and pH and salt responsiveness and can be used in the fields of gene transfer, gene therapy, drug controlled release, tissue engineering and the like.

Description

technical field [0001] The invention belongs to the technical field of polymer hydrogel, and relates to a method for preparing DNA base-pair cross-linked supramolecular hydrogel. Background technique [0002] With the establishment of supramolecular science, supramolecular hydrogels have also begun to attract widespread attention. Compared with traditional chemically cross-linked hydrogels, the three-dimensional network structure of supramolecular hydrogels is induced by weak interactions. Weak interactions are sensitive to external stimuli and often have certain reversibility. Therefore, supramolecular hydrogels not only maintain the properties of traditional hydrogels such as high water content, biocompatibility, and environmental friendliness, but also often have stress Response, self-healing and other excellent properties. At present, the hydrogen bonding in supramolecular hydrogels is mostly intermolecular hydrogen bonding in non-biological organisms, while the hydroge...

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Problems solved by technology

[0003] At present, the reports about base pair hydrogels in the literature all introduce base pairs into one or both ends of the macromolecular chain segments in the hydrogel system to form hydrogen bonds between the chain segments, but this type of hydrogel The main cross-linking effect of the glue is the directional arrangement and stacking effect between the chain segments, which has the disadvantage of unstable structure

Moreover, most of these hydrogels are gelled in organic solvents and then removed with deionized water. However, there will be solvent residues after washing, and organic solvents are irritating and toxic to the human body and will cause adverse reactions. This limits the biological applications of such hydrogels

[0004] Polyacrylamide hydrogel has certain biocompatibility and can be applied to human soft tissue filling materials. It has been approved for cosmetic operations such as breast augmentation and hip augmentation, but its structure is unstable, and it cannot maintain its original shape after long-term water absorption and swelling. shape, and become a sol state, which limits its application range to a large extent

[0005] Chinese patent (CN103694426A) describes a modified base pair introduced into the end of the polymer, through the formation of complementary base pairs between two bases, the hydrogen bond formed between the base pairs has salt and pH Responsiveness, although the polymer can be used in seawater desalination and personal health care, etc., but the invention has the disadvantages of unstable structure and slow response to salt and pH due to low base content

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