Exiguobacterium mexicanum 2a and applications thereof

A microbacterium, CCTCCM2014674 technology, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of equipment wear, small application range of mechanical wax removal, large investment of thermal wax removal, etc., to achieve strong anti-fluid erosion ability, strong microbial anti-wax Function, the effect of reducing the viscosity of crude oil

Active Publication Date: 2016-05-11
陕西博秦生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The scope of application of mechanical wax removal is small, the operation is difficult, and the equipment is seriously worn out, so it is rarely used; the thermal wax removal is expensive, the maintenance cost is high, and the effect is not ideal; the applica...

Method used

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  • Exiguobacterium mexicanum 2a and applications thereof
  • Exiguobacterium mexicanum 2a and applications thereof
  • Exiguobacterium mexicanum 2a and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The identification of embodiment 1 bacterial classification

[0029] (1) Morphological characteristics

[0030] The strain 2a was cultured on the peptone medium of beef extract. The colony of strain 2a was large and dry, orange, with surface folds raised upwards, and the edges were irregular; under the electron microscope, the cells were long rod-shaped, 0.3-0.5 μm wide and 1.0-2.0 μm long μm. Specific forms such as figure 1 .

[0031] (2) Biochemical properties

[0032] Gram staining is positive, aerobic, glucose and sucrose fermentation are positive, citric acid and malonic acid utilization are positive, ethanol oxidation and methyl red test are positive, V-P test is negative, nitrate reduction test, nitrous acid reduction And denitrification test are positive, urease test is negative, ammonia gas can be hydrolyzed, and starch and gelatin can be hydrolyzed.

[0033] (3) 16SrDNA sequence analysis

[0034] Through PCR reaction, a 1.6kb gene fragment was amplified ...

Embodiment 2

[0037] The application of embodiment 2 strains

[0038] (1) Degradation of crude oil

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PUM

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Abstract

The invention belongs to the technical field of tertiary oil recovery utilizing microbes and metabolism thereof to improve the crude oil yield, and discloses a strain of exiguobacterium mexicanum 2a, which is preserved in China Center for Type Culture Collection. The preservation number of the strain is CCTCC M2014674. The invention also discloses applications of exiguobacterium mexicanum 2a. The strain can be used to degrade crude oil or solid paraffin and can also be used to prevent crystal deposition of paraffin. The maximal degradation rate of colloids and asphalt in crude oil can reach 72.8% and 57.9% respectively, the viscosity of crude oil can be reduced by 11.1%, the solubility of solid paraffin in n-hexane is prominently improved, the soluble paraffin accounts for 62.0 % of the total weight of paraffin; compared with a control group, the crystallized paraffin is reduced by 64.7%; the wax clearing rate and wax preventing rate reaches 77.1% and 96.9% respectively; the cells of exiguobacterium mexicanum 2a are highly hydrophobic, the cells can tightly adhere on the surface of metals to form a biological membrane, and thus the crystal deposition of wax is prevented.

Description

technical field [0001] The invention belongs to the technical field of tertiary oil recovery by utilizing microorganisms and metabolites thereof to enhance crude oil recovery, and specifically relates to Microbacterium mexicans 2a and its application. Background technique [0002] Oil is the blood of the industrial economy, and oil security is related to a country's economic and social sustainable development and national defense security. In the international environment of economic globalization and regional development, all countries in the world regard the continuous, reasonable and stable acquisition of oil resources as an important national strategy. [0003] At present, through primary and secondary oil recovery, the recovery rate of crude oil is about 30% of geological reserves, and there is still a large amount of residual crude oil to be exploited by new technologies. The main factors that limit the increase of crude oil recovery: first, high molecular hydrocarbon...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K8/582C12R1/07
CPCC09K8/582C12N1/205C12R2001/07
Inventor 张俊会薛泉宏高卉来航线王平
Owner 陕西博秦生物工程有限公司
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