Myrothecium roridum and application thereof
A technology of lacquer spot fungus and conidia, which is applied in the field of microbial control, can solve problems such as environmental pollution, low toxicity, and few large-scale industrial applications, and achieve good control effects, rapid pathogenicity, and significant economic and economic benefits. The effect of social benefits
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Embodiment 1
[0029] Embodiment 1: the isolation and identification of dewy paint spot bacteria BC-1
[0030] 1.1 Isolation of strains
[0031] Collect naturally susceptible barnyardgrass leaves in the field, rinse twice with sterile water, cut off the diseased and healthy joints of the diseased leaves, and disinfect the surface with 5% (v / v) sodium hypochlorite solution for 2 minutes, and then use 70% ( v / v) Alcohol surface disinfection for 1 minute, after rinsing with sterile water, transfer these tissues to an empty sterilized petri dish, and dry them in an ultra-clean bench. Spread the dried leaf tissue on the surface of the PDA medium, place it in an incubator at 28°C, and transfer it to a new PDA culture dish after the bacteria grow, produce spores, separate and purify the single spores to obtain pure culture bacteria Strain BC-1. The BC-1 strain was stored in a -20°C refrigerator by the filter paper method.
[0032] 1.2 Identification of strains
[0033] 1.2.1 Morphological ident...
Embodiment 2
[0045] Example 2: Determination of the host range of bacterial strain BC-1
[0046] Prepare the spore suspension with reference to the method in 1.3, and spray it on the corn, wheat and rice plants at the 2-3 leaf stage. After 7 days, observe the safety of the strains on the tested corn, wheat and rice. After 21 days, the aerial parts of the crops were collected, the fresh weight was weighed, and the fresh weight inhibition rate was counted.
[0047] It is clear from the determination of the host range that the strain BC-1 is safe to crops such as corn, wheat and rice; it has a good biocontrol effect on barnyard grass, and the fresh weight inhibition rate after two weeks is about 91%. It has a certain biocontrol effect on weeds such as crabgrass and foxtail.
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