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Active aldehyde group modified substrate applied to microarray and preparation method of active aldehyde group modified substrate

A technology of aldehyde group modification and microarray, which is applied in the fields of medicine and biology to achieve uniform surface, high probe immobilization rate, and improved immobilization force

Inactive Publication Date: 2016-01-06
KUNMING HUANJI BIOLOGICAL CHIP IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a microarray with active aldehyde group modified substrate and its preparation method in order to solve the deficiency that the existing gene chip uses glass as the carrier

Method used

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  • Active aldehyde group modified substrate applied to microarray and preparation method of active aldehyde group modified substrate
  • Active aldehyde group modified substrate applied to microarray and preparation method of active aldehyde group modified substrate
  • Active aldehyde group modified substrate applied to microarray and preparation method of active aldehyde group modified substrate

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Embodiment 1

[0043] A method for preparing a microarray with an active aldehyde group-modified substrate, comprising the steps of:

[0044] Step (1), pretreatment of the slides: clean the slides once with a plasma cleaner for 5 minutes, then soak and wash the slides with concentrated sulfuric acid with a mass concentration of 98% for 1 hour, and then clean the slides with purified water. Wash the residual concentrated sulfuric acid on the surface to obtain the pretreated glass slide;

[0045] Step (2), poly-lysine coating: Coat the surface of the pretreated glass slide obtained in step (1) with poly-lysine, when coating poly-lysine, use a mass concentration of 0.5% The polylysine aqueous solution soaked the slides for 10 minutes, then washed off the excess uncoated polylysine on the surface of the slides with purified water, dried at 55°C for 30 minutes, cooled to room temperature, and obtained Polylysine-coated slides;

[0046] Step (3), introducing active aldehyde groups; using glutara...

Embodiment 2

[0048] A method for preparing a microarray with an active aldehyde group-modified substrate, comprising the steps of:

[0049] Step (1), pretreatment of the slides: the slides were first cleaned continuously with a plasma cleaner for 5 times, each time for 5 minutes, and then soaked in concentrated sulfuric acid with a mass concentration of 98% for 2 hours, and then the slides were cleaned with purified water. Wash the residual concentrated sulfuric acid on the surface to obtain the pretreated glass slide;

[0050] Step (2), poly-lysine coating: Coat the surface of the pretreated glass slide obtained in step (1) with poly-lysine, when coating poly-lysine, use a mass concentration of 1.5% The polylysine aqueous solution soaked the slides for 30 minutes, then washed off the excess uncoated polylysine on the surface of the slides with purified water, dried at 65°C for 60 minutes, cooled to room temperature, and obtained Polylysine-coated slides;

[0051] Step (3), introducing a...

Embodiment 3

[0053] A method for preparing a microarray with an active aldehyde group-modified substrate, comprising the steps of:

[0054] Step (1), pretreatment of the slides: the slides were first cleaned by a plasma cleaner for 3 consecutive times, each time for 3 minutes, and then soaked in concentrated sulfuric acid with a mass concentration of 98% for 1.6 hours, and then the slides were cleaned with purified water. Wash the residual concentrated sulfuric acid on the surface to obtain the pretreated glass slide;

[0055] Step (2), poly-lysine coating: Coat the surface of the pretreated glass slide obtained in step (1) with poly-lysine, when coating poly-lysine, use a mass concentration of 1.2% The polylysine aqueous solution soaked the slides for 18 minutes, then washed off the excess uncoated polylysine on the surface of the slides with purified water, dried at 60°C for 45 minutes, cooled to room temperature, and obtained Polylysine-coated slides;

[0056] Step (3), introducing ac...

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Abstract

The invention relates to an active aldehyde group modified substrate applied to a microarray and a preparation method of the active aldehyde group modified substrate, and belongs to the technical field of medicine and biology. A biological chip substrate is a substrate which is characterized in that an amination surface is connected with an aldehyde group. According to the aldehyde group substrate provided by the invention, the substrate is treated by concentrated sulfuric acid and a plasma washing machine, so that the problem of multiple impurity points of the common substrate can be solved; polylysine is firstly prepared into the amination surface, then glutaraldehyde is used as an aldehyde group donor; through combined action of electrostatic adhesion and chemical coupling, the fixing force of nucleic acid is improved; each probe point is full in shape; the stability of the substrate is improved; therefore, the aldehyde group substrate is an excellent biological chip probe carrier and can be widely applied to the preparation of biological chips.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a biochip substrate and a preparation method thereof, in particular to an active aldehyde group-modified substrate for a microarray and a preparation method thereof. Background technique [0002] Today, biochips have become an indispensable tool for life science and medical research and testing. Because of their high throughput, high sensitivity, and high accuracy, biochips can be used in the analysis of gene mutations, gene expression profiles, protein reactions, and pathogen detection. and other fields are widely used. The substrate is the basis of the chip, and its preparation is a very important link in the development of the biochip. [0003] Biochips generally choose correspondingly treated silicon chips, glass chips, metal chips, plastic chips or nitrocellulose membranes as carriers. Glass slides are widely used due to their cheapness, smooth surface, and low ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C40B40/06C40B50/18C12Q1/68C03C17/28
Inventor 马明星王超
Owner KUNMING HUANJI BIOLOGICAL CHIP IND
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