A kind of alternative Wallella and its application for remediating environmental heavy metal pollution

A technology of walnutella and heavy metals, which is applied in the field of heavy metal pollutant treatment and microorganisms, can solve problems such as pollution, threat to ecological environment safety, etc., and achieve the effects of reducing heavy metal content, improving practical value and application prospect.

Inactive Publication Date: 2018-03-09
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The natural environment contains a certain concentration of chromium, cadmium and lead, but with the rapid development of mining area development, electroplating, printing and dyeing, chemical industry, electronics and other industries, heavy metals such as chromium, cadmium and lead enter the atmosphere, water and soil environment excessively, causing serious pollution , threatening the safety of the ecological environment

Method used

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  • A kind of alternative Wallella and its application for remediating environmental heavy metal pollution
  • A kind of alternative Wallella and its application for remediating environmental heavy metal pollution
  • A kind of alternative Wallella and its application for remediating environmental heavy metal pollution

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Experimental program
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Effect test

Embodiment 1

[0022] The isolation and identification of embodiment 1 bacterial strain

[0023] 1) Sample collection: In July 2009, the ore soil samples used in the present invention were collected from the Tonglushan Copper-Iron Mine in Daye, Hubei.

[0024] 2) Sample enrichment: Weigh 20g of soil sample into a 200ml sterilized Erlenmeyer flask, add 1ml of 0.1M Cr(VI) mother liquor and mix well, so that the concentration of Cr(VI) in the soil is 0.5mM. Then seal the mouth of the triangular flask with the lid of the petri dish, place it in a 37°C incubator for enrichment for about a week, and add sterile water every two days during the enrichment process to ensure that the sample is not dry.

[0025] 3) Isolation of Cr(VI)-resistant bacteria: Weigh 10g of Cr(VI)-enriched soil sample in a Erlenmeyer flask filled with 100ml of sterile normal saline (0.85%), shake at 37°C for 20min, and take 1mL of soil suspension Dilute gradually to 10 in 9mL sterile normal saline -2 , 10 -3 , 10 -4 , 10 ...

Embodiment 2

[0027] Embodiment 2 indoor simulation test

[0028] 1) Preparation of liquid LB medium: Weigh 15 g of yeast powder extract (OXOID company, UK), 30 g of tryptone (OXOID company, UK), 30 g of NaCl, dissolve in 3 L of double distilled water, and make it completely After dissolving, put it in a sterilizing pot at 121°C and sterilize for 30 minutes.

[0029] 2) Pick a single colony of WH16-1 in 10ml LB liquid medium, culture it on a shaker at 37°C until the cell density is OD 600 is about 1. Add Pb(NO 3 ) 2 Pb 2+ The concentration is 100mg / L, then inoculate 100mL of the above-mentioned liquid LB medium according to the inoculation amount of 1%, culture in a shaker at 37°C and 150r / min, take 2mL samples after a period of time, centrifugally filter, and measure Pb in the filtrate 2+ concentration. After 96 hours, Pb in solution 2+ The removal rate was 84.13%.

[0030] 3) Pick a single colony of WH16-1 in 10ml LB liquid medium, culture it on a shaker at 37°C until the cell den...

Embodiment 3

[0033] Embodiment 3 soil simulation test

[0034] 1. Application of remediation of soil cadmium pollution

[0035] 1) Select paddy field soil, remove surface weeds, then air-dry, grind, pass through a 40-mesh sieve, and collect for future use; the pH of the soil is 6.05, and the organic matter content is 7.5g kg -1 , available phosphorus 2.75mg·kg -1 , and the moisture content was 24.65%.

[0036] 2) Prepare 6 black boxes of 250mL size, weigh 100g of air-dried soil into each box, and then add 0.001g of CdCl 2 2.5H 2 O, mix well, then add 20mL deionized water to each box, balance for three days, and control the water content to 20-30%.

[0037] 3) After balancing, spray WH16-1 bacteria solution evenly on the soil surface in three of the boxes, and the amount of bacteria solution is based on 1.0×10 8 cfu / g (soil) to convert; for the treatment without bacteria, replace it with the same amount of deionized water, mix well, weigh and record the weight, and then place it in a c...

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Abstract

The invention discloses an alternative Alishewanella sp. WH16-1 for repairing heavy metal pollution in the environment, and the preservation number is: CCTCC NO: M 2015073. The bacterial strain provided by the present invention can convert lead or cadmium in the environment from a carbonate binding state and an ion exchange state to a residue state, an organic binding state and an iron-manganese oxide binding state, or reduce chromium from a hexavalent state to a trivalent state. Valence state, thereby greatly reducing the biological activity of environmental heavy metals and reducing the heavy metal content in crops.

Description

technical field [0001] The invention belongs to the technical field of heavy metal pollutant treatment and microorganisms, and in particular relates to an alternative Alishewanella sp. and its application in repairing heavy metal pollution in the environment. technical background [0002] The natural environment contains a certain concentration of chromium, cadmium and lead, but with the rapid development of mining area development, electroplating, printing and dyeing, chemical industry, electronics and other industries, heavy metals such as chromium, cadmium and lead enter the atmosphere, water and soil environment excessively, causing serious pollution , threatening the safety of the ecological environment. [0003] Chromium cadmium lead has strong biological toxicity. For example, after lead and its compounds are absorbed into the body, they form soluble lead hydrogen phosphate, glycerol phosphate compounds or combine with proteins to circulate in the blood. Serious dam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 廖水姣王革娇周高婷夏险王辉
Owner HUAZHONG AGRI UNIV
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