Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of biological membrane obtained by natural source and/or self-assembly technology, and closed structure or cellular compartment with biological membrane property as medicine carriers

A self-assembly technology, cell compartment technology, applied in the application field of closed structure and cell compartment as a medical carrier

Inactive Publication Date: 2015-09-09
HANGZHOU UMOTOR BIOTECH CO LTD
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the deficiencies of artificial membranes that still have application defects at present, an object of the present invention is to provide biofilms obtained by natural sources and / or self-assembly techniques, closed structures with biofilm properties or cell compartments as pharmaceutical carriers. Application; Another object of the present invention is to provide pharmaceutical composition

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of biological membrane obtained by natural source and/or self-assembly technology, and closed structure or cellular compartment with biological membrane property as medicine carriers
  • Application of biological membrane obtained by natural source and/or self-assembly technology, and closed structure or cellular compartment with biological membrane property as medicine carriers
  • Application of biological membrane obtained by natural source and/or self-assembly technology, and closed structure or cellular compartment with biological membrane property as medicine carriers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 Extraction and purification of biofilm

[0076] The desired biofilm was extracted and purified by density gradient centrifugation. The specific process is as follows:

[0077] (1) New Zealand white rabbits that had been fasted for 18-24 hours were sacrificed to take the liver, and after removing the large blood vessels, the liver was cut into 2mm 3 The left and right small pieces were washed with normal saline until the tissue pieces turned white;

[0078] (2) Preparation of homogenate: 1mmol / L CaCl 2 , 50mmol / L HEPES (pH 7.4), 1mmol / L PMSF, 2μg / mL Aprotinin, 2μg / mL Antipain;

[0079] (3) Add liver tissue and homogenate to 8 times the volume of homogenate according to the mass:volume ratio, and homogenize with an electric homogenizer at 15,000 rpm in an ice-bath environment until the tissue is liquefied;

[0080] (4) Filter the homogenate through four layers of gauze, centrifuge the filtrate at 1,000×g, 10min, 4°C, and collect the precipitate;

[0081] ...

Embodiment 2

[0083] Example 2 Extraction and purification of biofilm

[0084] The desired biofilm was obtained by extracting and purifying by two-phase partition method. The specific process is as follows:

[0085] (1) Select plump and consistent normal corn seeds, soak them in 1% NaClO for 10 minutes, rinse them, and germinate them at 25°C in constant temperature and darkness for 72 hours;

[0086] (2) Take corn epicotyl, add 2 times the volume of extraction buffer (5mmol / L EDTA, 25mmol / L Tris, 0.25mmol / L sucrose, 1mmol / L MgSO 4 , 0.2% (W / V) BSA, 0.5% (W / V) PVP-10, 10% (W / V) glycerol, 15mmol / L β-mercaptoethanol, 1mmol / L PMSF, 1mmol / L DTT), ice bath grinding to liquefaction;

[0087] (3) Filter the grinding solution through four layers of gauze, centrifuge the filtrate at 12,000×g, 15min, 4°C, and take the supernatant;

[0088] (4) Centrifuge the supernatant at 80,000×g for 30 minutes at 4°C to collect the precipitate;

[0089] (5) Precipitate with suspension (25mmol / L Tris, 0.25mmo...

Embodiment 3

[0091] Example 3 Extraction and purification of biofilm

[0092] The desired biofilm was obtained by extracting and purifying by differential centrifugation. The specific process is as follows:

[0093] (1) Antarctic ice algae ( Chlamydomonas subcaudata ) is isolated and purified from Antarctic sea ice;

[0094] (2) Inoculate Antarctic ice algae into the medium at a ratio of 1:100 (21.2g NaCl, 3.6g NaSO per 10L medium 4 , 0.6g KCl, 0.3g NaHCO 3 , 0.1g KBr, 0.1g H 3 BO 3 , 0.1g NaF, 9.6g MgCl 2 ·6H 2 O, 1.0 g CaCl 2 , 0.1g SrCl 2 ·6H 2 O), cultured in a controlled light incubator. Cultivate for 14 days at -4°C, with a light intensity of 1300~1900lx, a light cycle of 12 light / 12 dark, and shaking 4~5 times a day;

[0095] (3) Centrifuge the ice algae culture solution at 4,000rpm, 20min, and 4°C to collect the ice algae sediment, and rinse it twice quickly with pre-cooled distilled water to remove extracellular viscous, surface salt and impurities in the culture so...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to application of a biological membrane obtained by a natural source and / or a self-assembly technology, and a closed structure or a cellular compartment with biological membrane property as medicine carriers. The biological sources of the biological membrane and the closed structure or the cellular compartment with the biological membrane property are animals, plants or microorganisms; the obtained biological membrane is intact or segmented, and is of a lipid bilayer structure in form; the constituents mainly are lipid and protein; and in addition, a small amount of saccharides are combined with the lipid or protein through covalent bonds. According to the biological membrane and the closed structure or the cellular compartment (hereinafter referred to as biological membrane) with the biological membrane property disclosed by the invention as the medicine carriers, the targets of improving the stability, the solubility and the curative effects of medicines can be reached; the toxicity or irritation is reduced; and the long-term effect and the targeting property are slowly released.

Description

technical field [0001] The invention belongs to the fields of biomedicine and polymer materials, and specifically relates to the application of biofilms obtained from natural sources and / or self-assembly, closed structures with biofilm properties, and cell compartments as medical carriers. Background technique [0002] Biomembranes or biological membranes are the general term for all membrane structures at the junction of cells, organelles and their environment. They play a role in dividing and separating cells and organelles, and are also important parts related to intracellular communication. All organisms have biofilms except some viruses. In addition to the plasma membrane (also known as the cell membrane), eukaryotic cells also have a membrane system that separates various organelles, including nuclear membranes, mitochondrial membranes, endoplasmic reticulum membranes, lysosome membranes, Golgi membranes, chloroplast membranes, vacuoles, and percutaneous membranes. Ox...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K47/46A61K8/99A61K8/98A61K8/97A61K39/187A61L27/36A61K31/704
Inventor 高颖姚震祥魏建良王旻子
Owner HANGZHOU UMOTOR BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com