A kind of method for extracting lincomycin by resin adsorption fermentation stock solution

Abstract

The invention discloses a method for extracting lincomycin lincomycin by using resin to adsorb a fermentation stock solution. The method comprises the following steps: (1), placing a fermentation solution in a pretreatment tank, adding a sodium hydroxide solution whiling stirring, heating, adding resin and stirring slowly to obtain filtered resin; (2) placing the filtered resin into alkaline water with the same volume, and adding deionized water to clean the resin; (3), placing the cleaned resin in a chromatographic column and eluting the resin with n-butyl alcohol; (4), taking lincomycin desorbed solution and adding activated carbon for decolorizing; (5) desalinating the decolorized liquid through a desalinating column to obtain lincomycin desalinating liquid; (6), concentrating; (7) drying to obtain the finished product of hydrochloric acid lincomycin. The method provided by the invention avoids the procedures of plate-frame pressure filtration or ceramic membrane filtration, solvent extraction, back extraction and the like in the traditional process, and has the advantages of being simple in operation, low in labor intensity, clean in working environment, simplified in process, small in occupied area, low in cost and small in environmental pollution; the total recovery is greater than 85%, and the purity of the finished product is greater than 98%.

Description

technical field [0001] The invention relates to a method for separating and purifying high-purity lincomycin, belonging to the technical field of antibiotic purification. Background technique [0002] Lincomycin is a broad-spectrum antibiotic produced by the fermentation of Streptomyces lincolnensis. It mainly acts on the 50S subunit of the ribosome of sensitive bacteria, preventing the elongation of the peptide chain, thereby inhibiting the protein synthesis of bacterial cells. There is no cross-resistance between lincomycin and other antibiotics, and its own toxicity is low. It can also be used as a precursor to synthesize clindamycin and other antibiotics. It can be used clinically for patients allergic to penicillin and erythromycin, various infections caused by sensitive bacteria, such as pneumonia, meningitis, endocarditis, cellulitis, tonsillitis, erysipelas, furuncle and urinary system infection, etc. Mycin is widely used in the medical industry and occupies an imp...

AI Technical Summary

Problems solved by technology

[0005] 1. The process is lengthy and the total yield is low

[0006] 2. The plate and frame filter press is operated intermittently, with low efficiency, high labor intensity, and poor sanitation conditions in the working environment

[0007] 3. Emulsified during extraction, the solvent is highly water-soluble, difficult to recycle, waste water cannot be treated, and causes serious environmental pollution

[0008] 4. The amount of organic solvent used in crystallization is large, and the operating cost is high

However, none of them can fundamentally solve the problems raised in 15, and with the improvement of equipment, equipment input costs and operating costs have been greatly improved, and are not suitable for industrialized large-scale production.

[0010] In the technical scheme disclosed in Chinese invention patent application publication number [CN104356179 A] and publication number [CN101348508 A], it is proposed to use resin to adsorb lincomycin, and n-butanol is resolved, but publication number [CN104356179 A] and publication number [ CN101348508 A] None extract lincomycin from the fermentation stock solution, all need to be extracted, all need acetone recrystallization, the process is still lengthy, the total yield is low, and it is also unsuitable for industrial production