A kind of preparation method of dihydromyricetin-loaded ternary composite liposome
A technology of dihydromyricetin and ternary compounding, which is applied in the directions of liposome delivery, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., can solve problems such as macrocytotoxicity, and maintain biological activity. , reduce toxicity and improve the efficiency of transfection
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Embodiment 1
[0022] A preparation method of dihydromyricetin-loaded ternary composite liposomes, comprising the following steps:
[0023] (1) Dissolve dihydromyricetin, phosphatidylglycerol (PG) and dioleoylphosphatidylethanolamine (DOPE) in chloroform at a molar ratio of 2:3:3, evaporate under reduced pressure to remove chloroform, and obtain dry lipid Membrane, add HEPES buffer (pH7.4), shake slowly to suspend the lipid film in HEPES buffer to obtain anionic liposome suspension, put the anionic liposome suspension in an ultrasonic cleaner for Ultrasound in a water bath, fully hydrated, and squeezed through the membrane to obtain anionic liposomes;
[0024] (2) Dissolve trimethyl-2,3-dioleoyloxypropylammonium bromide (DOTAP) and phosphatidylcholine (PC) in chloroform at a molar ratio of 1:1, and evaporate under reduced pressure to remove chloroform , to obtain a uniform film, add HEPES buffer (pH7.4), slow shaking makes the lipid film suspended in HEPES buffer, to obtain cationic liposom...
Embodiment 2
[0027] A preparation method of dihydromyricetin-loaded ternary composite liposome, said method comprising the following steps:
[0028] (1) Dissolve dihydromyricetin, phosphatidylinositol (PI) and cholesterol (CHOL) in chloroform at a molar ratio of 2.5:3.5:3.5, evaporate under reduced pressure to remove chloroform, obtain a dry lipid film, and add HEPES Buffer solution (pH7.4), shake slowly to suspend the lipid film in the HEPES buffer solution to obtain anionic liposome suspension, place the anionic liposome suspension in an ultrasonic cleaner for ultrasonic cleaning in a water bath, fully After hydration, squeeze through the membrane to obtain anionic liposomes;
[0029] (2) Dissolve 1,2-dioleoyl-3-succinyl-sn-glycerol choline ester (DOSC) and dioleoylphosphatidylethanolamine (DOPE) in chloroform at a molar ratio of 1:0.8, and decompress Evaporate and remove chloroform to obtain a uniform film, add HEPES buffer (pH7.4), slowly shake to suspend the lipid film in HEPES buffe...
Embodiment 3
[0032] A preparation method of dihydromyricetin-loaded ternary composite liposome, said method comprising the following steps:
[0033](1) Dissolve dihydromyricetin, phosphatidylserine (PS) and dioleoylphosphatidylethanolamine (DOPE) in chloroform at a molar ratio of 2.8:4:3, evaporate under reduced pressure to remove chloroform, and obtain dry lipid Membrane, add a certain volume of HEPES buffer (pH7.4), shake slowly to suspend the lipid film in HEPES buffer to obtain anionic liposome suspension, put the anionic liposome suspension in ultrasonic cleaning Ultrasound in a water bath in the instrument, after fully hydrating, squeeze through the membrane to obtain anionic liposomes;
[0034] (2) Dissolve trimethyl-2,3-dioleyloxypropyl ammonium chloride (DOTMA) and phosphatidylcholine (PC) in chloroform at a molar ratio of 1.2:1, and evaporate under reduced pressure to remove chloroform , to obtain a uniform film, add HEPES buffer (pH7.4), slow shaking makes the lipid film suspen...
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