A composite bilayer film capable of selectively releasing biomolecules regulated by an electric field and its preparation method

Abstract

The invention discloses a composite double-layer film capable of regulating and controlling selective release of biomolecules through an electric field and a preparation method thereof. The film is continuously deposited through an electrochemical deposition method, the lower layer is a traditional doped polypyrrole film layer, and the upper layer is a polypyrrole composite layer which contains two kinds of biomolecules co-deposition; and the thickness and the composition of the upper layer and the lower layer can be regulated and controlled through the electrochemical deposition electric quantity and the deposition electric current and time. The film has good biocompatibility, in view of different changes of the biomolecules caused by the change of the pH near electrodes of the biomolecules and the large difference of the particle size and the electronegativity between the two kinds of biomolecules, selective release of the two kinds of in-situ biomolecules can be regulated and controlled through different voltages, and related growth factors needed by cells at different periods are provided for the cells at a fixed time and location; and the preparation process of the film is simple and feasible, the film can be widely applied to the fields of cell culture in vitro, tissue engineering, medical instrument surface coatings and the like.

Description

technical field [0001] The invention belongs to the field of biomedical thin films, and in particular relates to a composite double-layer thin film capable of selectively releasing biomolecules under the control of an electric field and a preparation method thereof. Background technique [0002] Traditional polypyrrole, as a conductive polymer that has been widely studied in recent years, has the characteristics of simple preparation, uniform shape, controllable composition, and good biocompatibility [X.H.Chu, Q.Xu, Z.Q.Feng, et al.In vitrobiocompatibility of polypyrrole / PLGA conductive nanofiber scaffold with cultured rat hepatocytes.Materials Research Express, 2014,1(3):035402.], the polymerization method of pyrrole is simple, the monomer loses an electron on the electrode surface and is oxidized to a positive atomic group , followed by the generation of dimers, trimers, and multimers of positive atomic groups. There is electrostatic repulsion between the positive atomic ...

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Problems solved by technology

[0003] However, the polypyrrole film layer deposited with biomolecules will greatly reduce the biocompatibility [D.D.Ateh, P.Vadgama, H.A. Navsaria. Culture of human keratinocytes on polypyrrole-basedconducting polymers. :645-655.], after research, it is due to the reduction of the polypyrrole polymerization degree caused by biomolecules as a dopant, and the polypyrrole with a low polymerization degree is more cytotoxic

[0004] Therefore, a new preparation method for depositing biomolecular polypyrrole films is imperative, and different growth factors are required in different stages of cell growth, and a single biomolecule deposition cannot meet the needs of cell growth. The emergence of in situ timed selective growth factor films has great significance for in vitro cell culture, tissue engineering and other biomedical engineering fields

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