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A composite bilayer film capable of selectively releasing biomolecules regulated by an electric field and its preparation method

A biomolecular and composite double-layer technology, applied in the direction of electrolytic organic material coating, etc., can solve the problems of reduced polymerization degree of polypyrrole, decreased biocompatibility of polypyrrole film layer, and high cytotoxicity of polypyrrole.

Active Publication Date: 2020-01-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the polypyrrole film layer deposited with biomolecules will greatly reduce the biocompatibility [D.D.Ateh, P.Vadgama, H.A. Navsaria. Culture of human keratinocytes on polypyrrole-basedconducting polymers. :645-655.], after research, it is due to the reduction of the polypyrrole polymerization degree caused by biomolecules as a dopant, and the polypyrrole with a low polymerization degree is more cytotoxic
[0004] Therefore, a new preparation method for depositing biomolecular polypyrrole films is imperative, and different growth factors are required in different stages of cell growth, and a single biomolecule deposition cannot meet the needs of cell growth. The emergence of in situ timed selective growth factor films has great significance for in vitro cell culture, tissue engineering and other biomedical engineering fields

Method used

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  • A composite bilayer film capable of selectively releasing biomolecules regulated by an electric field and its preparation method
  • A composite bilayer film capable of selectively releasing biomolecules regulated by an electric field and its preparation method
  • A composite bilayer film capable of selectively releasing biomolecules regulated by an electric field and its preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Dissolve the traditional dopant in deionized water at a concentration of 20mg / ml first, wait for it to completely dissolve, add pyrrole monomer at a concentration of 0.1M, and prepare the lower layer deposition solution;

[0031] 2) Dissolve the biomolecule BMP-2 and the biomolecule heparin sodium in deionized water in turn, the concentrations are 0.02mg / ml and 5mg / ml, respectively, after they are completely dissolved, add pyrrole monomer, the concentration is 0.1M, and configure the upper layer deposition fluid;

[0032] 3) Put the substrate into the underlying deposition solution as the working electrode, use the Pt electrode as the counter electrode, and use the chronopotentiometry method to deposit on the substrate with a current of 1mA for 60s, and deposit the lower conventionally doped polypyrrole film layer;

[0033] 4) Transfer the substrate with the lower polypyrrole film layer directly to the deposition solution containing biomolecules and deposit it on the...

Embodiment 2

[0036] 1) First dissolve the traditional dopant in deionized water at a concentration of 1mg / ml, wait until it is completely dissolved, add pyrrole monomer at a concentration of 0.2M, and prepare the lower layer deposition solution;

[0037] 2) Dissolve biomolecules BMP-2 and biomolecules hyaluronic acid in deionized water in turn, the concentrations are 0.04mg / ml and 1mg / ml, respectively, after they are completely dissolved, add pyrrole monomer, the concentration is 0.2M, and configure supernatant liquid;

[0038] 3) Put the substrate into the underlying deposition solution as the working electrode, use the Pt electrode as the counter electrode, and use the chronopotentiometry method to deposit on the substrate with a current of 0.1mA for 300s, and deposit the lower conventionally doped polypyrrole film layer;

[0039] 4) The substrate with the lower polypyrrole film layer is directly transferred to the deposition solution containing biomolecules and deposited on the substrat...

Embodiment 3

[0041] 1) Dissolve the traditional dopant in deionized water at a concentration of 50mg / ml first, wait for it to completely dissolve, add pyrrole monomer at a concentration of 0.5M, and prepare the lower layer deposition solution;

[0042] 2) Dissolve biomolecule albumin and biomolecule hyaluronic acid in deionized water in turn, the concentrations are 2mg / ml and 0.5mg / ml respectively, after they are completely dissolved, add pyrrole monomer, the concentration is 0.5M, and configure the upper layer deposition fluid;

[0043] 3) Put the substrate into the underlying deposition solution as the working electrode, use the Pt electrode as the counter electrode, and use the chronopotentiometry method to deposit on the substrate with a current of 2mA for 10s, and deposit the lower conventionally doped polypyrrole film layer;

[0044] 4) Transfer the substrate with the lower polypyrrole film layer directly to the deposition solution containing biomolecules and deposit on the substrate f...

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Abstract

The invention discloses a composite double-layer film capable of regulating and controlling selective release of biomolecules through an electric field and a preparation method thereof. The film is continuously deposited through an electrochemical deposition method, the lower layer is a traditional doped polypyrrole film layer, and the upper layer is a polypyrrole composite layer which contains two kinds of biomolecules co-deposition; and the thickness and the composition of the upper layer and the lower layer can be regulated and controlled through the electrochemical deposition electric quantity and the deposition electric current and time. The film has good biocompatibility, in view of different changes of the biomolecules caused by the change of the pH near electrodes of the biomolecules and the large difference of the particle size and the electronegativity between the two kinds of biomolecules, selective release of the two kinds of in-situ biomolecules can be regulated and controlled through different voltages, and related growth factors needed by cells at different periods are provided for the cells at a fixed time and location; and the preparation process of the film is simple and feasible, the film can be widely applied to the fields of cell culture in vitro, tissue engineering, medical instrument surface coatings and the like.

Description

technical field [0001] The invention belongs to the field of biomedical thin films, and in particular relates to a composite double-layer thin film capable of selectively releasing biomolecules under the control of an electric field and a preparation method thereof. Background technique [0002] Traditional polypyrrole, as a conductive polymer that has been widely studied in recent years, has the characteristics of simple preparation, uniform shape, controllable composition, and good biocompatibility [X.H.Chu, Q.Xu, Z.Q.Feng, et al.In vitrobiocompatibility of polypyrrole / PLGA conductive nanofiber scaffold with cultured rat hepatocytes.Materials Research Express, 2014,1(3):035402.], the polymerization method of pyrrole is simple, the monomer loses an electron on the electrode surface and is oxidized to a positive atomic group , followed by the generation of dimers, trimers, and multimers of positive atomic groups. There is electrostatic repulsion between the positive atomic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G73/06C25D9/02C25D5/54
CPCC08G73/0611C25D5/54C25D9/02
Inventor 程逵朱翼飞翁文剑
Owner ZHEJIANG UNIV
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