Method of screening medicaments with high accuracy and high repeatability

Abstract

The invention provides a method of screening medicaments with high accuracy and high repeatability. The method comprises the following steps: 1) preparing a patterned fiber electrospinning receiving board, and preparing a receiving board with a patterned metal silver layer by utilizing a photoetching technology and a direct current magnetron sputtering technology; 2) preparing patterned electrospinning fibers, and carrying out electrostatic spinning on an object obtained in the step 1); 3) preparing a PDMS cavity; 4) connecting the objects obtained in the second and third steps by virtue of plasma treatment; and 5) inoculating primary mouse myocardial cells to the patterned electrospinning fibers in the object obtained in the step 4), pumping a culture medium into the cavity of the object obtained in the step 4) by virtue of an injection pump, then pumping the medicaments, recording the beating frequency of the myocardial cells within 20 days after the medicaments are pumped to obtain an EC50 value, and screening the medicaments according to the EC50 value. According to the method disclosed by the invention, normal growth of the cells in smaller cavity is realized, and the method also has the effects of screening the medicaments and reusing and is high in the accuracy and the repeatability.

Description

Technical field [0001] The invention belongs to the field of drug screening, and specifically relates to a drug screening method with high accuracy and high repeatability. technical background [0002] Drug screening is to screen out highly effective new drugs or lead compounds from natural or synthetic compounds. The process of drug screening is the process of conducting drug activity experiments on compounds, and the activity experiments on compounds have gradually changed from early confirmatory experiments to screening experiments. It is an important part of the modern drug development process to test and obtain specific physiologically active compounds. step. [0003] In the prior art, two-hybrid drug selection technology, recombinant receptor drug selection technology, and transgenic animal model drug selection technology all have the disadvantages of complicated technical steps, high detection cost, and poor accuracy. [0004] In recent years, chip drug screening technol...

AI Technical Summary

Problems solved by technology

However, the chips in the prior art all have the problems of high processing difficulty, scarcity of raw materials or high cost of raw materials, making it difficult for drug screening chips to be widely used.

[0005] In particular, the use of electrospun fibers to prepare drug screening systems, especially chip screening systems, has always been faced with the technical problems that it is difficult to prepare a specific array of electrospun fibers, and it is difficult to fit the electrospun fibers to the size of the chip.

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