Genetic engineering recombinant high-purity anti-mycotoxin monoclonal antibody for inhibiting and killing mycotoxin as well as composite monoclonal antibody preparation method and combined preparation thereof

Abstract

The invention relates to a genetic engineering recombinant high-purity anti-mycotoxin monoclonal antibody for inhibiting and killing mycotoxin as well as a composite monoclonal antibody preparation method and a combined preparation thereof. The preparation method comprises the following steps: screening mycotoxin; connecting the mycotoxin with vector carrier to prepare immune antigen; performing immune and cell fusion to prepare a hybridoma cell; performing gene cloning, constructing anti-mycotoxin monoclonal antibody Ig and expressing in engineering bacteria; fermenting and amplifying recombinant engineering bacteria, culturing to prepare bacteria of the anti-mycotoxin monoclonal antibody Ig; performing cell crushing, filtering and removing bacteria and viruses, centrifuging, purifying and performing chromatography to prepare anti-mycotoxin monoclonal antibody Ig and soluble antibody protein of composite monoclonal antibody IgC. The anti-mycotoxin monoclonal antibody and the composite monoclonal antibody IgC can be used as a grain mycotoxin remover, feed components and can be prepared into an animal drug; the anti-mycotoxin monoclonal antibody Ig, the composite monoclonal antibody IgC and the combined preparation thereof can be used for effectively degrading the mycotoxin in feed and food, preventing and treating mycotoxin toxic disorders.

Description

technical field [0001] The present invention relates to a new method for suppressing mycotoxins in food, feed and food, more specifically, to a method for preparing mycotoxin-inhibiting genetically engineered recombinant high-purity anti-mycotoxin monoclonal antibody and compound monoclonal antibody and its combination preparation. Background technique [0002] In recent years, mycotoxin poisoning has brought more and more harm to animal husbandry. According to a survey by the Food and Agriculture Organization of the United Nations, 25% of the world's traded grains are contaminated with mycotoxins. In my country, the proportion of feed and raw materials contaminated with mycotoxins exceeds the standard as high as 60% to 70%. According to statistics, more than 66% of feed materials are contaminated with at least one mycotoxin, 35% of feed materials are contaminated with more than two types of mycotoxins, almost all peanut meal is contaminated with mycotoxin, and corn meal, ...

AI Technical Summary

Problems solved by technology

[0009] 4. Oxidation treatment method: the treatment effect is unstable, and the nutrients such as vitamins in the feed are seriously lost

[0010] 5. Antioxidants: Aflatoxin poisoning cannot be completely eliminated, and the concentration of antioxidants such as BHT exceeds 30 times the conventional use to show a better detoxification effect; in this way, it will inevitably have a toxic effect on animals

[0011] 6. High-temperature treatment method: high energy consumption and great damage to the nutrients in the feed, so there are few practical applications

The ideal reaction conditions for detoxification enzymes are in vitro reaction systems with neutral pH value and high moisture content, which are suitable for vegetable oil, soy sauce, beer, milk, etc. It is difficult to directly apply them in low moisture feed and raw materials

[0014] In view of the fact that countries in the world have so far no ideal method to deal with mycotoxins in feed and food, there is no safe and effective feed additive for degrading mycotoxins, and there is no specific drug for the treatment of mycotoxin poisoning. Even the most prestigious [Mycotoxin antidote] produced in Germany only adopts the traditional dextran adsorption method; market feedback information shows that the existing methods have failed to solve the increasingly serious problem of mycotoxin pollution

Therefore, the harm of mycotoxins is becoming more and more serious; it has brought huge economic losses to the feed industry and animal husbandry production in my country and the world and damaged national health

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