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Method for separation and preparation of alkaloids from walnut green seedcase

A technology of walnut green skin and alkaloids, applied in the separation/purification of carboxylic acid compounds, organic chemistry and other directions, can solve the problems of incomplete separation, low purity, low extraction rate, etc., and achieve the effect of strong adaptability

Inactive Publication Date: 2015-04-01
LULIANG UNIV
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Problems solved by technology

Common alkaloid extraction methods include cold soaking, percolation, ultrasonic, Soxhlet extraction, heat reflux extraction, etc., but there are problems of low extraction rate, incomplete separation and low purity

Method used

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  • Method for separation and preparation of alkaloids from walnut green seedcase
  • Method for separation and preparation of alkaloids from walnut green seedcase
  • Method for separation and preparation of alkaloids from walnut green seedcase

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Embodiment Construction

[0027] The above and other technical features and advantages of the present invention will be described in more detail below in conjunction with the accompanying drawings.

[0028] The invention uses green walnut peel as raw material, and adopts multistage extraction and HSCCC to separate and prepare alkaloids after being assisted by an ultrasonic microwave extractor. The results show that: use methanol water (4:1) as the extractant, the ratio of liquid to material is 10:1, after assisted treatment by ultrasonic microwave extractor for 15min, the chloroform methanol water alkaloid extract is obtained after multi-stage extraction; (16:1) was used as a developing agent, and clearer multiple monomer images were obtained by TLC; ) were collected for each monomer at 313nm. Conclusion: Combined with the characteristics of ultraviolet spectrum, the collected monomers were compared with a variety of alkaloid standard samples for TLC images, and four monomers were preliminarily confir...

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Abstract

The invention relates to a method for separation and preparation of alkaloids from walnut green seedcase, the alkaloids is separated and prepared by multistage extraction and HSCCC (high speed counter-current chromatography) after auxiliary processing of the walnut green seedcase as a raw material by an ultrasonic microwave extraction device, a mixture with the ratio of methanol: water of 4: 1 is used as an extraction agent, the material liquid ratio is 10 to 1, after 15min of auxiliary processing by the ultrasonic microwave extraction device, a chloroform methanol water alkaloid extraction solution can be obtained by multistage extraction; according to the HSCCC method, chloroform and methanol are used, a solid phase carrier is not required, the loss caused by irreversible adsorption and degradation of a sample in the solid phase carrier can be eliminated, recovery rate is high, sampling can be repeated, the separation process can keep stable, reproducibility is good, gradient elution and reversed-phase elution can be realized; repeated sampling can be realized, separation efficiency is high, and the separation amount is large.

Description

technical field [0001] The invention relates to the field of separation and preparation of alkaloids, in particular to a method for separation and preparation of alkaloids from walnut green peel. Background technique [0002] Walnut (Juglans regia L) Qingpi, also known as Qinglongyi, is a thick immature green peel on the outside of walnut. It contains alkaloids, naphthoquinones, flavonoids, diarylheptanes, polysaccharides, small molecule terpenes, etc. Alkaloids are an important class of nitrogen-containing organic substances derived from the biological kingdom (mainly plants), and mainly exist in various organs and tissues in plants. Plants of the same family and genus may contain alkaloids of the same structure type, and the content in certain organs of the plant may be higher than that in other tissues of walnuts. Among walnut tissues, the alkaloids in fresh green skin were significantly higher than those in other tissues. [0003] The content of alkaloids in plants is...

Claims

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Application Information

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IPC IPC(8): C07D471/22C07D451/10C07D221/22C07D455/03C07D311/62C07C65/03C07C51/47C07C51/42
CPCC07C51/42C07D221/22C07D311/62C07D451/10C07D455/03C07D471/22C07C65/03
Inventor 杨卫民张苏勤杜京旗赵君刘宝琦
Owner LULIANG UNIV
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