Technology for enzymatic efficient deacetylation and chitin degradation by using Thermophilic Bacillus

A thermophilic bacillus, deacetylation technology, applied in the fields of biopharmaceutical technology, chemical engineering, and food processing technology, can solve the problems of difficult product purification, easy pollution of the environment, and large energy consumption

Inactive Publication Date: 2015-03-25
ZHEJIANG SHUREN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

These methods all use inorganic strong bases or strong acids, which have the disadvantages of high energy consumpti

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  • Technology for enzymatic efficient deacetylation and chitin degradation by using Thermophilic Bacillus
  • Technology for enzymatic efficient deacetylation and chitin degradation by using Thermophilic Bacillus
  • Technology for enzymatic efficient deacetylation and chitin degradation by using Thermophilic Bacillus

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Embodiment Construction

[0010] A technology for efficient deacetylation of chitin by using Thermophilic Bacillus enzyme

[0011] 1. Preparation of strains

[0012] 1) Plate culture: Bacillus thermophilus C8-3-2a, ctn C8-1, ctn C8-1`, ctn x10, ctn x5-3 were cultured on a double-layer plate with chitin as the sole carbon source, respectively. Streak inoculation on the base, culture at 45-46°C for 24 hours, and put it in the refrigerator for later use; 2) Liquid culture: Use an inoculation loop to pick out the cultured plates of Bacillus thermophilus C8-3-2a, ctnC8- 1. Inoculate ctn C8-1`, ctn x10, ctn x5-3 strains into 100 mL of liquid medium with chitin as the only carbon source, culture at 45-46 °C for 72 hours, and store it in a refrigerator for later use;

[0013] 3) Raw material treatment: The chitin used to prepare the plate medium should be ground first, and then passed through a 30-mesh sieve. Chitin for fermentative production is cut into small pieces or small particles.

[0014] 2. Ferment...

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Abstract

A technology for enzymatic efficient deacetylation and chitin degradation by using Thermophilic Bacillus belongs to the fields of the food processing technology, the bio-pharmaceuticals and the chemical engineering technology. The above strain has the biological characteristic of generation of highly active extracellular chitin deacetylase and chitin degrading enzyme, and can efficiently degrade chitin. The technology mainly comprises the following steps: inoculating preserved slant strain into a double layer plate with chitin as a sole carbon source, culturing at 46DEG C for 24h, inoculating a certain amount of the above obtained substance into a liquid medium with chitin as the sole carbon source, carrying out liquid seed culturing at 46DEG C for 72h, and inoculating the obtained production strain into a fermentation medium with chitin as the sole carbon source according to a certain ratio. The technology can degrade chitin to produce chitosan, chitosan oligosaccharide with different molecular weights, glucosamine (aminoglucose), acetylglucosamine and other relevant products, and also can produce chitin deacetylase and the chitin degrading enzyme.

Description

technical field [0001] The invention relates to a technology for efficiently deacetylating chitin by utilizing Thermophilic Bacillus enzyme, belonging to the fields of food processing technology, biopharmaceutical technology and chemical engineering technology. Background technique [0002] Zhejiang Province is a major marine province in my country, and the aquaculture industry plays an important role in the economic development of the province. The annual output of shrimp and crabs in mariculture exceeds 60,000 tons, and the shrimp and crabs in ocean fishing exceed 800,000 tons. Chitin is a by-product with a large output in aquatic products processing, with an annual output of more than 50,000 tons. The pollution caused by the processing of chitin is very serious. Chitin is widely found in shrimp, crab and other crustaceans, some plants, bacteria and fungi and other organisms. It is the second largest natural polymer compound after cellulose, with an average annual output ...

Claims

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Application Information

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IPC IPC(8): C12P19/26C12N9/88C12N9/42C12R1/01
Inventor 活泼林荣业李成平饶桂维梅瑜王楠金建昌严小平朱晓娟
Owner ZHEJIANG SHUREN UNIV
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