Application of herba lysimachiae total flavonoids in preparation of medicine for relieving hyperuricemia

A technology of hyperuricemia and buckwheat grass, applied in the directions of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problem of no clear active ingredients, no feasibility, etc., and achieve clear ingredients, reduce high uric acid, and reduce serum uric acid. Effect

Active Publication Date: 2014-12-24
CATCH BIO SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in Wei's research, only the water extract was used for drug efficacy evaluation, and its active ingredients were not clarified. At the same time, the drug efficacy evaluation dose reached 12.5g / kg, far exceeding the dosage of the finished medicine, so it is not feasible

Method used

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  • Application of herba lysimachiae total flavonoids in preparation of medicine for relieving hyperuricemia
  • Application of herba lysimachiae total flavonoids in preparation of medicine for relieving hyperuricemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of total flavonoids of Desmodium

[0029] (1), take 5 kilograms of Desmodium syringae dry whole herb, grind it, soak it with 50 liters of 70% v / v ethanol aqueous solution at room temperature for one day, reflux extraction for 60 minutes, after filtering out the extract, add 50 liters of 70% ethanol to the filter residue v / v ethanol aqueous solution, reflux extraction for 40 minutes, and filter. The two extracts were combined and concentrated to dryness in vacuo to obtain 400 g of Desmodium chinensis crude extract with a yield of 8%.

[0030] (2) Take 50g of the crude extract of Desmodium desmodium prepared in step (1), dissolve it in water, and perform chromatography on Diaion HP-20 macroporous adsorption resin. The ratio of the mass of the extract to the mass of the resin is 1:20. Elute with water, 30% v / v ethanol water, 60% v / v ethanol water and 95% v / v ethanol water, each gradient elution is 4-5 column volumes, and the flow rate is 2-3 column v...

Embodiment 2

[0031] Example 2 Desmodium Desmodium Crude Extract and Determination of Total Flavonoids in Ethanol Elution Parts

[0032] Preparation of reference substance stock solution: Accurately weigh 20 mg of rutin reference substance, put it in a 100ml volumetric flask, add 60% ethanol to dissolve and dilute to the mark, shake well to obtain rutin stock solution (0.2 mg / mL).

[0033] Preparation of standard curve: pipette 0.0, 1.0, 2.0, 3.0, 4.0, 5.0ml of rutin stock solution into six 25mL volumetric flasks, add distilled water 7.0, 6.0, 5.0, 4.0, 3.0, 2.0mL respectively, Shake well, add 1.0mL of 5% sodium nitrite, shake well, stand for 6min, add 1.0mL of 10% aluminum nitrate solution, shake well, let stand for 6min; add 10mL of 4% sodium hydroxide solution, add distilled water to constant volume, shake After the color was developed for 15 minutes, its absorbance A was measured at a wavelength of 510nm. With least squares linear regression, the regression equation of solution absorba...

Embodiment 3

[0040] Example 3 Analysis of Components of Total Flavonoids of Desmodium Desmodium in the Elution Part of 60% Ethanol

[0041] Preparation of the test solution: accurately weigh 26.1mg of the sample and place it in a 25ml volumetric flask, dissolve it with 80% methanol and set the volume to the mark for analysis.

[0042] HPLC detection conditions:

[0043] Liquid phase: Agilent1260DAD

[0044] Column: AX-09

[0045] Column temperature: 30°C

[0046] Injection volume: 5μl

[0047] Flow rate: 1ml / min

[0048] Detection wavelength: 190-400nm full scan (extract 274nm spectrum)

[0049] Mobile phase: B (acetonitrile)-D (2% acetic acid-water), gradient elution, see Table 2.

[0050] Table 2 Gradient elution schedule

[0051] time (min)

B[％]

D[％]

0

15

85

3.30

15

85

5.00

20

80

11.67

90

10

13.33

90

10

15.00

15

85

20.00

15

85

[0052] Result: HPLC det...

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Abstract

The invention relates to an application of herba lysimachiae total flavonoids in preparation of a medicine for relieving hyperuricemia. The content of total flavonoids in the herba lysimachiae total flavonoids is more than 60%, and the herba lysimachiae total flavonoids contain 7.0wt% to 10.0wt% of kaempferol, 6.0wt% to 9.0wt% of quercetin, 5.0wt% to 9.0wt% of rutin, 7wt% to 10wt% of epicatechin and 7.0wt% to 10wt% of hesperidin. The herba lysimachiae total flavonoids are high in total flavonoid content and definite in ingredients, and also have the function of obviously lowering serum uric acid of a model rat with the hyperuricemia under the condition of the total flavonoids in a low dose, so that the herba lysimachiae total flavonoids are high in safety and are of a good medicine for lowering high uric acid and treating gout. In addition, the herba lysimachiae total flavonoids are simple in preparation process and are suitable for industrial production and applications.

Description

technical field [0001] The invention relates to a preparation method and application of plant extract total flavonoids for preparing medicine for treating hyperuricemia. Background technique [0002] In recent years, due to the increase in people's stress and the increase in intake of high-calorie, high-protein, and high-fat foods, the number of people suffering from hyperuricemia or gout symptoms caused by it has increased sharply. It is reported that about 20% of Adult males are in a state of hyperuricemia. [0003] In general, a state in which the uric acid level in the blood is 7.0 mg / dL or more is called hyperuricemia. Usually, a simple hyperuricemia state has no subjective symptoms, but if this state is left alone for a long time, urate in the blood crystallizes, and when this urate accumulates in joints, etc., it can cause gout accompanied by severe pain attack. If the above symptoms develop further, gouty nodules will appear under the skin or uric acid crystals wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K36/53A61K31/7048A61P19/06
Inventor 温尧林姚譞夏增华
Owner CATCH BIO SCI & TECH
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