Amphipathy photoswitch fluorescent polymer nano particles and preparing method thereof

Abstract

The invention discloses amphipathy photoswitch fluorescent polymer nano particles and a preparing method of the amphipathy photoswitch fluorescent polymer nano particles. Methyl methacrylate, polymerizable green fluorescence dye and a polymerizable photochromic compound are taken as monomers, hexadecane is taken as hydrophobe, a polyoxyethylene macromolecular chain transfer reagent is taken as a surface active agent, a one-step reversible addition-fragmentation chain transfer (RAFT) miniemulsion polymerization method is adopted for preparing the novel amphipathy photoswitch fluorescent polymer nano particles. The amphipathy photoswitch fluorescent polymer nano particles can emit intense macroscopic green or red fluorescence under irradiation of visible light and ultraviolet light, and have fast photoresponse capacity and good repeated fluorescence reversible switch performance. The amphipathy photoswitch fluorescent polymer nano particles have wide application prospects in the fields of cell imaging, bioanalysis, optical storage and the like.

Description

technical field [0001] The invention relates to the field of material preparation, in particular to amphiphilic light-switchable fluorescent polymer nanoparticles with fluorescence reversible switch performance under the irradiation of ultraviolet light and visible light and a preparation method thereof. Background technique [0002] In recent years, optically switchable fluorescent materials have received increasing attention in the fields of ultra-high-resolution cell imaging, labeling of complex biological systems, and ultra-high-density optical data storage applications. For example, optical switching fluorescent materials based on spiropyran derivatives, diarylethene derivatives, etc. have good optical rewritable repeatability and low production cost, which provides a low-cost optical ultra-high density storage technology. new possibilities. On the other hand, compared with conventional fluorescent probes, probe materials with optical switch fluorescence characteristic...

AI Technical Summary

Problems solved by technology

However, among these existing preparation methods, the sol-gel method has harsh synthesis conditions and poor reproducibility; the nanoparticle surface modification method and the block copolymer self-assembly method require complex preparation processes, which are not conducive to large-scale Preparation and commercial production; while the conventional micro / miniemulsion method requires the use of common ionic or nonionic surfactants, instability due to migration or aggregation of surfactants will occur in complex physiological environments

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