Kit for detecting mosquito borne pathogens and detection method thereof

Abstract

The invention discloses a kit for detecting a plurality of mosquito borne pathogens and a detection method thereof. The plurality of mosquito-borne infectious pathogens are detected by using multiplex polymerase chain reaction (PCR) combined with a liquid chip, and a yellow fever virus (YFV), dengue fever viruses (DV) I-IV type, epidemic encephalitis B (japanese encephalitis) virus (JEV), plasmodium (Plasmodium), (including plasmodium vivax (PV), plasmodium falciparum (PF), plasmodium malariae (PM), plasmodium ovale (PO)), a west nile virus (MNV) and a chikungunya virus (CHIKV) can be detected in parallel one time. The kit has the advantages of being large in flux, high in specificity and sensitivity, stable in result, good in repeatability, simple to operate and fast in detection speed.

Description

technical field [0001] The invention belongs to the technical field of biochips and diagnostic reagents, in particular to a kit and a detection method for detecting various mosquito-borne pathogens, which design specific nucleic acid probes for various mosquito-borne infectious disease pathogens and react with multiple polymerase chain reactions Combining (PCR) technology and using liquid-phase chips to quickly and synchronously detect a variety of mosquito-borne pathogens and their typing. Background technique [0002] Mosquito-borne infectious diseases refer to diseases transmitted by mosquitoes biting the human body, and have been on the rise year by year in recent years. Once a mosquito-borne infectious disease becomes popular or breaks out, it will have a great impact on social stability, people's health, life and production. According to the needs of infectious disease screening at border ports, yellow fever, dengue fever, Japanese encephalitis (Japanese encephalitis)...

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Problems solved by technology

However, there are more or less defects in these methods: for example, the isolation and culture method is technically difficult, and it usually takes more than 3 to 5 days to obtain the results of etiological diagnosis and drug susceptibility, and it is difficult to carry out etiological diagnosis and drug resistance early in clinical practice. However, it cannot meet the needs of clinical treatment of critically ill infected patients; when immunological methods are used to detect virus-specific antibodies, it is not only necessary to collect double sera in the acute phase and recovery phase of the patient's infection, but also there may be crossover between antigens and antibodies. In terms of nucleic acid detection methods, PCR, multiplex PCR, RT-PCR methods, and real-time fluorescent PCR methods based on Taqman probe technology have high sensitivity and good specificity, and are suitable for rapid detection. However, most of these methods are based on the detection of a single reaction or a small number of multiple reactions. When testing clinical samples, it takes a lot of labor to detect and exclude dozens of indicators in each sample. and cost

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