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A silk fibroin gel microneedle system and preparation method thereof

A silk fibroin and gel technology, which is applied to a microneedle system composed of a silk fibroin solidified product and its preparation field, can solve problems such as loss of drug activity, and achieve the effects of simple method and mild processing conditions.

Active Publication Date: 2016-03-30
PHARSUN MEDICAL BIOTECHNICS (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these insolubilization processes may cause some loss of drug activity

Method used

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  • A silk fibroin gel microneedle system and preparation method thereof
  • A silk fibroin gel microneedle system and preparation method thereof
  • A silk fibroin gel microneedle system and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] (1) Mold production: select a PDMS film with a thickness of about 2mm, and set the laser conditions as follows: a carbon dioxide laser with a power of 30 watts, a focusing aperture of 100 μm, a carbon dioxide laser power of 5W, a frequency of 3000, and a dotting time of 5ms. Square array, each hole is 500 μm apart, the mold made under this laser drilling condition, the microneedle length is 450 μm, and the diameter of the base is about 250 μm.

[0021] (2) Preparation of silk fibroin solution: Take 80g of silkworm raw silk and put it into 5000ml of 0.06% sodium carbonate solution, boil it three times at 98-100°C, use deionized water for three times, and treat each time for 30min to remove the raw silk The sericin in the fiber is washed, loosened and then dried in an oven at 60°C to obtain pure silk fiber. Dissolve the dried silk fiber in 9.3M lithium bromide solution at 65°C, the bath ratio is 3:20, and the dissolution time is about 1 hour. After it cools down, take it ...

Embodiment 2

[0024] (1) Mold production: select a PDMS film with a thickness of about 1.5mm, set the laser conditions as follows: 30W carbon dioxide laser, focusing aperture 200μm, power 15W, frequency 6000, dotting time 10ms, punch out 10*10 hole square array Array, each hole is separated by 500 μm, the mold made under this laser drilling condition has a micro-length of 700 μm and a base diameter of about 250 μm.

[0025] (2) Preparation of silk fibroin solution: Take 80g of silkworm raw silk and put it into 5000ml of 0.05% sodium carbonate solution, boil it three times at 98-100°C, use deionized water for three times, and treat each time for 30min to remove the raw silk The sericin in the fiber is washed, loosened and then dried in an oven at 60°C to obtain pure silk fiber. Dissolve the dried silk fiber in 9.3M lithium bromide solution at 65°C, the bath ratio is 3:20, and the dissolution time is about 1 hour. After it cools down, take it out and put it into a dialysis bag and place it in...

Embodiment 3

[0028] (1) Mold production: Select a PDMS film with a thickness of about 2mm, set the laser conditions as follows: carbon dioxide laser, focusing aperture 300μm, power 27W, frequency 9000, dotting time 20ms, punch out a square array of 15*15 holes, each hole With a distance of 600 μm, the mold made under this laser drilling condition has a microneedle of 1000 μm and a base diameter of about 250 μm.

[0029](2) Preparation of silk fibroin solution: Preparation of silk fibroin solution: Take 80g of silkworm raw silk and put it into 5000ml of sodium carbonate solution with a concentration of 0.06%. After processing for 30 minutes, the sericin in the raw silk is removed, washed, loosened, and dried in an oven at 60°C to obtain pure silk fiber. Dissolve the dried silk fiber in 9.3M lithium bromide solution at 65°C, the bath ratio is 3:20, and the dissolution time is about 1 hour. After it cools down, take it out and put it into a dialysis bag and place it in deionized water for dia...

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Abstract

The invention discloses a silk fibroin gel microneedle system and a manufacturing method of the silk fibroin gel system microneedle system. Silk fibroin solution with the mass concentration between 3 percent to 15 percent is processed through ultrasonic oscillation and then is poured on a polydimethylsiloxane film needle hole die array obtained through laser boring, dies are placed in a vacuum drying box and vacuumized, the solution is made to enter holes, the vacuum degree is kept, the dies are dried to constant weight, and then the silk fibroin gel microneedle system is obtained. The microneedle system is an insoluble gel microneedle and has certain brute force to puncture into the skin, the microneedle system swells after making contact with body fluid in the skin, and medicine in the microneedle system is released. The gel microneedle system is good in biocompatibility, easy to prepare, moderate in condition and capable of biodegradation, and the system can be used in the aspects of cosmetology, transdermal drug absorption devices and the like.

Description

technical field [0001] The invention relates to a medical microneedle system, in particular to a microneedle system composed of insoluble silk fibroin solidified material and a preparation method thereof. Background technique [0002] With the development of micro-processing technology, the micro-needle technology that can pierce the epidermis without hurting the dermis and nerves came out more than ten years ago, bringing new ideas for transdermal drug delivery. The microneedle lattice with a size of micron can pierce the extremely poorly permeable skin surface (stratum corneum) in a reversible and minimally invasive way, creating a mechanical channel through which biomacromolecules can pass without causing pain and skin trauma. However, the seemingly simple microneedle transdermal drug delivery still faces a series of technical bottlenecks. At present, the microneedles used for transdermal drug delivery must be prepared by micromachining technology, which is expensive and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61M37/00
CPCA61M37/0015A61M2037/0046A61M2037/0053
Inventor 卢神州侯静殷祝平朱天李明忠
Owner PHARSUN MEDICAL BIOTECHNICS (SHANGHAI) CO LTD
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