Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

New technology for preparing poria polysaccharide extract through membrane separation and purification technology

A Poria cocos polysaccharide and membrane separation technology, which is applied in the field of natural product extraction and separation, can solve the problems of low extraction rate and long extraction time, and achieve the effects of improving purity, shortening extraction time, and reducing the dissolution of impurities

Active Publication Date: 2014-05-07
WUHAN HUANG MEDICAL SYST BIOLOGICAL MEDICINE INC
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the current extraction and separation methods have the advantages of being cheap, non-toxic, and safe to operate, the extraction time is too long and the extraction rate is low, so it is impossible to obtain effective single-component pachyrhin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Get 5 kg of Poria cocos, put it into the extraction tank, add 30 kg of deionized water, cover and seal it, control the temperature at 90 ° C, and the auxiliary ultrasonic power is 10 MHz, stir intermittently for 5 minutes per hour, after leaching for 80 minutes, filter the filtrate, and then Add 10kg of deionized water for three times of extraction (boiling for 30 minutes each time), combine the four times of boiling and extracting liquid, filter out a total of 55kg after coarse filtration by centrifuge, and cool to 30°C; filter the filtrate with a microfiltration membrane with a pore size of 5 μm PP cotton filtration, operating temperature 25 ℃, pressure 0.3Mpa, obtain filtrate 51kg; Obtained filtrate is further filtered with polysulfone ultrafiltration membrane PS-10 with nominal molecular weight cut-off 10000 Dalton, operating temperature 25 ℃, pressure 0.05Mpa, obtain The filtrate was 49kg; the filtrate was concentrated and then freeze-dried to obtain 3.7kg of pachym...

Embodiment 2

[0031] Take 10kg of Poria cocos, 100kg of extractant, and put them into the extraction tank, control the temperature at 95°C, and the auxiliary ultrasonic power at 15 MHz, stir intermittently for 10 minutes per hour, leaching, after 100min, filter out the filtrate, and then add 50kg of extractant to extract for 100min , the two extracts were combined and then centrifugally filtered to remove impurities; 146kg of the filtrate was cooled to 30°C and then filtered with a microfiltration membrane PP cotton with a pore size of 10μm. The operating temperature was 30°C and the pressure was 0.5Mpa to obtain 143kg of the filtrate; The roll-type polypropylene ultrafiltration membrane HPS-30 with a molecular weight of 30,000 Daltons was further filtered at an operating temperature of 30° C. and a pressure of 0.2 Mpa to obtain 138 kg of filtrate; the filtrate was concentrated and freeze-dried to obtain 7.8 kg of saffron powder.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to a new technology for preparing poria polysaccharide extract through membrane separation and purification technology. The technology comprises the following steps: (1) grinding and sieving poria powder into crude powder with 20 meshes, and then adding five to ten times of deionized water to obtain feed liquor; (2) filtering the feed liquor obtained in the step (1) through a centrifugal machine; (3) filtering the filtrate obtained in the step (2) through a micro-filtering membrane PP cotton; (4) further filtering the filtrate through the polysulfone hollow fiber type ultrafiltration film with nominal molecular weight cutoff of 10000 dalton; (5) drying the filtrate obtained in the step (4) in a vacuum freeze-drying manner; (6) carrying out anti-oxidization and anti-aging research to the poria polysaccharide by taking a drosophila as an animal model; (7) carrying out hypoglycemic action research by adopting an in-vitro alpha-glucosidase inhibition model through the poria polysaccharide prepared in the step (5). The new technology is simple to operate, and capable of greatly increasing the purity and efficiency of polysaccharide.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction and separation. The invention relates to a method for extracting, separating and purifying polysaccharides prepared from poria cocos as a raw material, and the efficacy evaluation of hypoglycemic and antioxidative activities. Background technique [0002] Poria cocos belongs to the genus Poria cocos of the family Polyporaceae of the order Aphylloides, and is a fungal traditional Chinese medicine. Sweet, light, flat, heart-guiding, spleen, kidney meridian, has the effects of diuretic and dampness, invigorating the spleen and calming the heart. The main medicinal ingredient in Poria cocos is Poria cocos polysaccharide. Poria cocos polysaccharide has anti-tumor, anti-virus, anti-oxidation, enhancement of immunity, liver protection, hypnosis, anti-inflammation, anti-calculus and other effects, and can be widely used in medical care, food and other fields. Poria cocos polysacchari...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 何静仁洪峰刘莉段李歌蔡小双齐敏玉
Owner WUHAN HUANG MEDICAL SYST BIOLOGICAL MEDICINE INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com