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Cell micro-imaging method, image processing method and imaging analysis system

A technology of microscopic imaging and image processing, applied in image data processing, image enhancement, material analysis using radiation, etc., can solve the problems of neglecting influence, less research on image processing, lack of research on grating and photoresist, etc. To achieve the effect of reducing operation time and cost

Inactive Publication Date: 2014-04-30
NO 128 HOSPITAL OF HANGZHOU
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] In China, at present, soft X-ray microscopic imaging technology is mostly used in microbiology, plant and animal biological sample specimens, and the use of soft X-ray for cell microscopic imaging research is still in its infancy and initial stage, and the research focus is mainly on In terms of soft X-ray imaging operations, too much reliance on X-ray imaging itself, less research on sample processing and image processing, the impact of sample processing and image processing on image information is often ignored, and there is still a lack of research on the best suitable for Research on Diffraction Grating for Soft X-ray Imaging
In addition, due to the influence of traditional X-ray imaging techniques (such as hard X-ray imaging technology), less attention is paid to the folds that may occur during ultrathin sectioning. Microscopic imaging of cells can have a great impact; in the imaging process, traditional X-ray imaging technology is often used, and there is a lack of research on the best grating and photoresist for soft X-rays, resulting in the performance of diffraction gratings being affected. limit

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  • Cell micro-imaging method, image processing method and imaging analysis system
  • Cell micro-imaging method, image processing method and imaging analysis system
  • Cell micro-imaging method, image processing method and imaging analysis system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 : Method for soft X-ray imaging and treatment of suspected esophageal squamous cell carcinoma cells using soft X-ray microscopy

[0050] Select 6 hospitalized suspected patients (including 4 males and 2 females), aged 51-64 (average 57.3 years old).

[0051] The specimens were cut and sent to the pathology department for routine histological and cytological examinations. When suspected esophageal squamous cell carcinoma cells were found, light microscopic photos of the suspected esophageal squamous cell carcinoma cells were taken under an optical microscope with a magnification of 100 times. At the same time, samples of suspected esophageal squamous cell carcinoma cells were sent to the synchrotron radiation laboratory for soft X-ray microscopic beamline scanning diffraction, developed on photoresist, and soft X-ray microscopic images were taken.

[0052] The equipment used in this example is:

[0053] Use the soft X-ray microscopic imaging beamline station...

Embodiment 2

[0065] Example 2: Soft X-ray Imaging and Processing Method of Suspected Lung Cancer Cells Using Soft X-ray Microscopic Imaging

[0066] Suspected lung cancer patients were selected, male or female.

[0067] Experimental means such as instruments and equipment:

[0068] Soft X-ray Microimaging Beamline Station (National Synchrotron Radiation Laboratory, University of Science and Technology of China);

[0069] Vacuum drying oven, CJ-3A glue throwing machine (produced by Beijing Semiconductor Equipment Factory);

[0070] Olympus differential interference contrast microscope (Japan)

[0071] Scanning electron microscope KYKY-1000B (manufactured by the Scientific Instrument Factory of the Chinese Academy of Sciences);

[0072] 151II ultramicrotome (manufactured by SEIXY factory in West Germany).

[0073] Preparation of soft X-ray micrograph specimens of suspected lung cancer:

[0074] Step 1: Place suspected lung cancer tissue and cell specimens on a pathological ultrathin s...

Embodiment 3

[0087] Example 3 : Application of soft X-ray microscopy in inspection of plant leaves

[0088] Take wheat seeds, germinate the seeds in an incubator at 25°C, cut the leaves on the 7th-9th day, and cut them into 2mm 2 The slices are 10-20nm in thickness. The specimen is placed on the water surface of a 40°C water tank, so that the specimen floats on the water surface and spreads out quickly and evenly in a plane shape. The bath contains Tween 20 with a weight of 3ppm. The ultra-thin section specimens that have been floated and flattened in the water tank are picked up with a net, and then the electron microscope copper net is placed on absorbent paper to control the moisture and dried. The electron microscope copper net is 300 mesh and is processed as follows before the section is taken: Spread the clean copper grid on the filter paper in the petri dish, then slowly pour 0.3% by weight of Formvar solution until it covers the copper net, then drain the liquid in the petri dish...

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Abstract

The invention relates to a cell micro-imaging method, an image processing method and an imaging analysis system. The cell micro-imaging method is implemented by using a soft X ray and comprises the following steps of (1) preparing a sample; (2) primarily seeking and selecting a target cell; and (3) imaging the soft X ray. The image processing method is capable of reducing noises, eliminating artifacts and improving the contrast ratio. The imaging analysis system comprises an optical microscope system, a synchrotron radiation device, an image processing system and a display device. The methods and the system provided by the invention have the advantages that the existence and the region environment of a cell are effectively detected, the space scales, the structures and the functions of the cell and a sub-cell are researched, and thus the effective middle information is provided for multiple fields such as pathologies, medicines, agriculture and forestry and foods.

Description

[0001] technical field [0002] The invention relates to a method for microscopic imaging of cells by using soft X-rays, an image processing method and an imaging analysis system. [0003] Background technique [0004] X-rays discovered by Roentgen in 1895 have the property of strongly penetrating matter and are suitable for imaging human tissues and organs. Its wavelength is shorter, 0.1-1.0nm. However, the interaction between X-rays and matter is very complicated, and it is difficult to manufacture optical components similar to those used in visible light applications, so it is difficult to realize X-ray microscopes. With the birth of the electron collision synchrotron radiation source, special soft X-rays are emitted with a wavelength of 1-10nm. The soft X-rays are focused and projected onto the sample through optical diffraction and optical elements, and then imaged on the image plane. [0005] The resolution of soft X-ray microscopy has reached 10nm, the principle is...

Claims

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Application Information

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IPC IPC(8): G01N23/04G06T5/40
Inventor 郝建张帆董茂生罗毅吴升赵永飞王波
Owner NO 128 HOSPITAL OF HANGZHOU
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