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Preparation method of casein keratinase crosslinked polymer membrane

A keratinase and heteropolymer technology, which is applied in the field of preparation of casein keratinase cross-linked heteropolymer films, can solve problems such as environmental problems and insufficient modification effect, and achieves reduced production costs, good water resistance, and hydrophobicity. Sex-enhancing effects

Active Publication Date: 2014-04-30
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these modifications can improve the mechanical properties of keratin film formation to varying degrees, they also bring environmental problems, and the modification effect is not obvious enough.

Method used

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  • Preparation method of casein keratinase crosslinked polymer membrane
  • Preparation method of casein keratinase crosslinked polymer membrane
  • Preparation method of casein keratinase crosslinked polymer membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of casein keratinase cross-linked heteropolymer film, comprising the following steps:

[0035] 1) Preparation of casein and keratin solution

[0036] Preparation of casein solution: Take 7g casein, 92g water and 1g5mol / L NaOH solution, immerse the casein in part of deionized water, add NaOH solution, mix well, soak continuously for more than 24 hours (continuously soak during this process) Add part of deionized water until the casein is completely soaked, and the pH value of the system is kept below 7.0); stir the casein at this time evenly, and add the remaining deionized water. The pH value of the solution is adjusted to 7.5, and the magnetic stirring is continued until completely dissolved to obtain a casein solution with a mass fraction of 7%;

[0037] Preparation of keratin solution: Add 7g of keratin powder into 93g of deionized water, mix well, adjust the pH value of the system to 9.0 with solid NaOH, and stir magnetically for 3 hours at 50°...

Embodiment 2

[0045]A preparation method of casein keratinase cross-linked heteropolymer film, comprising the following steps:

[0046] 1) Preparation of casein and keratin solution

[0047] Preparation of casein solution: Take 7g casein, 92g water and 1g5mol / L NaOH solution, immerse the casein in part of deionized water, add NaOH solution, mix well, soak continuously for more than 24 hours (continuously soak during this process) Add part of deionized water until the casein is completely soaked, and the pH value of the system is kept below 7.0); stir the casein at this time evenly, and add the remaining deionized water. The pH value of the solution is adjusted to 7.5, and the magnetic stirring is continued until completely dissolved to obtain a casein solution with a mass fraction of 7%;

[0048] Preparation of keratin solution: Add 7g of keratin powder into 92.5g of deionized water, mix well, add 0.5g of solid NaOH to adjust the pH value of the system to 9.0, and stir magnetically for 4 h...

Embodiment 3

[0056] A preparation method of casein keratinase cross-linked heteropolymer film, comprising the following steps:

[0057] 1) Preparation of casein and keratin solution

[0058] Preparation of casein solution: Take 7g of casein, 92g of water and 1g of 5mol / L NaOH solution, immerse the casein in part of deionized water, add NaOH solution, mix well, soak continuously for more than 12h (continuously add part of the deionized water until the casein is completely soaked and the pH value of the system is kept below 7.0); stir the casein evenly at this time, and add the remaining deionized water. The pH value of the solution is adjusted to 6.5, and the magnetic stirring is continued until it is completely dissolved to obtain a casein solution with a mass fraction of 7%;

[0059] Preparation of keratin solution: Add 7g of keratin powder into 92.5g of deionized water, mix well, add 0.5g of solid NaOH to adjust the pH of the system to 9.0, and stir magnetically for 0.5h at 55°C until t...

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Abstract

The invention discloses a preparation method of a casein keratinase crosslinked polymer membrane, and belongs to the technical field of leather working. The method comprises the following steps: 1) mixing a casein solution with a keratin solution, and then adjusting the pH value of the mixed solution to 6.5-7.5, stewing to obtain a protein mixed solution; 2) adding glutamine transaminase transferase to the mixed solution according to the adding amount of 10-30U of transferase in each gram of dry matter protein, carrying out crosslinking reaction after fully and evenly mixing, and then inactivating, so as to obtain a mixed hybrid polymer of casein and keratin; 3) adding a film-forming agent to the mixed hybrid polymer of casein and keratin, and then molding, so as to obtain the casein keratinase crosslinked polymer membrane. The casein and the keratin are crosslinked under the catalytic action of the glutamine transaminase transferase, so as to form a covalent crosslinked macromolecule polymer. The crosslinked macromolecule polymer is improved in mechanical property, strong in water-resisting property, and good in biocompatibility, can be completely biodegraded, and has a broad application prospect in the fields of a zoology coating and a biological membrane.

Description

technical field [0001] The invention belongs to the technical field of leather processing, and in particular relates to a preparation method of a casein keratinase cross-linked heteropolymer film. Background technique [0002] Casein is one of the most widely used film-forming agents in leather finishing, and it is green and pollution-free. As a leather finishing agent, it has strong adhesion, is not easy to fall off due to friction, has good temperature resistance, light resistance, and has good It has the advantages of air permeability and water vapor permeability, but the casein coating also has disadvantages such as not being resistant to wet rubbing, brittle and hard, easy to break when bent, poor anti-corrosion ability, and prone to mildew. At home and abroad, casein modified by caprolactam and casein modified by graft copolymerization of vinyl monomers have been industrialized. In addition, some people have studied the modification of casein with silicone, polyurethan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C12P21/02C08L89/00
Inventor 王鸿儒苏小舟黄勉
Owner SHAANXI UNIV OF SCI & TECH
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