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Application of phyllanthus urinaria polysaccharide component in preparing drug for resisting hepatitis B virus

A bead polysaccharide and anti-hepatitis B technology, which is applied in the field of medicine, can solve the problems of less adverse reactions and low prices of Chinese herbal medicines that need further research, and achieve the effects of improving the body's immunity, high development value, and less dosage

Inactive Publication Date: 2013-04-17
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many varieties of traditional Chinese herbal medicines, which have been widely used in the clinical treatment of chronic hepatitis B and have achieved certain curative effects, and have accumulated rich clinical experience. However, the mechanism of Chinese herbal medicines against HBV needs to be further studied, so the screening of Chinese herbal medicines against HBV remains to be done. It has great potential; and the traditional Chinese herbal medicine has few adverse reactions and low price, and has been paid more and more attention by researchers at home and abroad. Seeking natural medicines with antiviral effects is a hot spot in current research

Method used

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  • Application of phyllanthus urinaria polysaccharide component in preparing drug for resisting hepatitis B virus
  • Application of phyllanthus urinaria polysaccharide component in preparing drug for resisting hepatitis B virus
  • Application of phyllanthus urinaria polysaccharide component in preparing drug for resisting hepatitis B virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Extraction of Phyllostachys polysaccharide

[0023] 1 Materials and methods

[0024] 1.1 Materials (omitted)

[0025] 1.2 Experimental method

[0026] 1.2.1 Phyllostachys pretreatment

[0027] The whole plant of Phyllostachys phylloxera was washed with distilled water to remove the soil, dried at 50°C, crushed, and sealed for later use. Its powder is yellow-green.

[0028] 1.2.2 Extraction method of Phyllostachys polysaccharide

[0029] Petroleum ether reflux at 80 °C to degrease → 95% ethanol at 80 °C to remove small molecule sugars → 90 °C water to reflux for three times, combine the filtrate for three times and centrifuge at 4000r / min, concentrate to 1 / 4 volume → add 4 times the volume of 95% ethanol , stand still for 24 hours, centrifuge, redissolve in distilled water → remove protein by sevag method (chloroform: n-butanol = 4:1, polysaccharide solution: mixed solution = 3:1), magnetically stir for 30 minutes, fully stand for stratification, repeat 7~...

Embodiment 2

[0059] Example 2 Separation and Purification of Phyllostachys polysaccharide

[0060] 1 Materials and methods

[0061] 1.1 Materials (omitted)

[0062] 1.2. Experimental method

[0063] 1.2.1 DEAE-52 column separation

[0064] 1.2.1.1 DEAE-52 filler pretreatment

[0065] Soak DEAE-52 cellulose filler in distilled water for 48 hours, change the water once every 4 to 5 hours, remove suspended impurities by pouring method, and then carry out alkali-acid-alkali treatment. First soak in 0.5 mol / L NaOH solution for 30 minutes, then wash with distilled water until neutral, then soak in 0.5 mol / L HCl solution for 30 minutes, then wash with distilled water until neutral, and finally soak in 0.5 mol / L NaOH solution for 30 minutes After washing with distilled water to neutrality, OH - type cellulose. Wet column packing (column size 500×50mm), equilibrated with distilled water for 48 hours, avoiding bubbles and faults during column packing.

[0066] 1.2.1.2 Separation and purificat...

Embodiment 3

[0086] Embodiment 3 Physicochemical properties and structural analysis of PULPⅡ

[0087] 1. Physical and chemical properties

[0088] PULPⅡ is a khaki powder, easy to absorb moisture. Soluble in water, insoluble in ethanol, acetone and other organic solvents. Molish reaction, phenol-sulfuric acid reaction, sulfuric acid-carbazole reaction are positive, ninhydrin reaction, iodine-potassium iodide reaction, Fehling reaction, ferric chloride reaction and sulfate group reaction are negative.

[0089] The physical and chemical properties, uronic acid content determination, elemental analysis, infrared and ultraviolet, and HPLC comprehensive analysis show that PULP II is a kind of acidic polysaccharide that does not contain N and S elements, and the relative average molecular weight is 579962.6.

[0090] 2. Infrared absorption spectrum of PULPⅡ

[0091] Infrared spectrum shows that PULP Ⅱ is at 3100~3500 cm -1 , 2800~2900 cm -1 , 1400~1530 cm -1 , 1000~1100 cm -1 There are obvi...

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Abstract

The invention relates to an application of a phyllanthus urinaria polysaccharide component in preparing a drug for resisting a hepatitis B virus, and belongs to the field of medicine. The phyllanthus urinaria polysaccharide component is a phyllanthus urinaria polysaccharide component PUIP II which comprises rhamnose, arabinose, mannose and glucose at a molar ratio of 0.11:0.36:0.08:0.45. The phyllanthus urinaria polysaccharide component is artificially extracted, separated and purified by a medicinal plant, namely phyllanthus urinaria. An in-vitro bioactivity experiment indicates that the phyllanthus urinaria polysaccharide component PUIP II can inhibit secretion of HBsAg and HBeAg and reproduction of HBV-DNA (hepatitis B virus-deoxyribose nucleic acid) in HepG2.2.2.15 cell culture, and has a certain in-vitro HBV activity resistance effect, and an in-vivo activity experiment indicates that the PUIP II has an obvious inhibiting effect on DHBVDNA (duck hepatitis B virus deoxyribose nucleic acid) in a duck, is smaller in bounce, and can be used for preparing the drug for resisting the hepatitis B virus and for preparing health care products for protecting a liver.

Description

technical field [0001] The invention relates to the application of the polysaccharide component of Phyllostachys japonicus in the preparation of anti-hepatitis B virus medicine, and belongs to the field of medicine. Background technique [0002] Hepatitis B Virus (HBV) has been discovered for more than 40 years. In 1963, Blumberg and others found that the serum of Australian hemophiliac patients contained an antigen that could react with the serum of American hemophiliac patients, calling it the Australian antigen (ie, Hepatitis B Virus Surface Antigen, Hepatitis B Virus Surface Antigen, HBsAg ). In 1970, Dane et al. found HBV particles with a size of 42nm in the serum of hepatitis B patients, and the outer membrane component contained HBsAg. In 1986, the International Committee on Virus Nomenclature officially classified HBV as a hepadnaviridae. [0003] Hepatitis B virus (HBV) infection is a worldwide health problem with worldwide distribution. It can not only cause acut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P31/20A23L1/30
Inventor 李清禄李凌峰李宇翔张丽丽谢勇平蔡向阳黄志坚
Owner FUJIAN AGRI & FORESTRY UNIV
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