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Inorganic/organic diphase nano composite bone tissue engineering scaffold and preparation method thereof

A bone tissue engineering and nano-composite technology, applied in medical science, prosthesis, etc., can solve the problems of difficult processing, difficult to stabilize material properties, difficult to degrade, etc., and achieve the effect of promoting cell adhesion, improving toughness, and good biological activity.

Inactive Publication Date: 2013-03-20
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) There are large differences in composition and microstructure from natural bone tissue, resulting in large differences in physical, chemical, biological and mechanical properties between the material and bone tissue, and the tissue and cell affinity of the material is not ideal;
[0006] (2) The speed of chemical bonding with bone tissue and the controllable degradability are not ideal. Some materials degrade too fast and others are difficult to degrade. The degradation speed of materials in the body is difficult to match with the growth speed of new bone;
[0007] (3) The dispersion of the inorganic phase in the composite scaffold is poor, and the interaction force with the organic phase is weak, which affects the mechanical properties of the composite scaffold;
[0008] (4) The material preparation process conditions are relatively complicated, and the material performance is difficult to stabilize, which causes difficulties in processing

Method used

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  • Inorganic/organic diphase nano composite bone tissue engineering scaffold and preparation method thereof
  • Inorganic/organic diphase nano composite bone tissue engineering scaffold and preparation method thereof
  • Inorganic/organic diphase nano composite bone tissue engineering scaffold and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0055] 1) Prepare 1.5mol / L soluble calcium salt solution and 1.8mol / L soluble phosphate solution; the contents of chitosan, chondroitin sulfate and hyaluronic acid are respectively 2%, 0.1% and 0.05% by mass percentage ;

[0056] 2) dissolving the chitosan in the ratio in 1% acetic acid solution, and dissolving the chondroitin sulfate / hyaluronic acid in the ratio in deionized water;

[0057] 3) Slowly add the chondroitin sulfate / hyaluronic acid mixture into the chitosan acetic acid solution, stir to make it fully mixed;

[0058] 4) According to n(Ca 2+ ):n(PO 4 3- )=1.67:1 ratio, a certain amount of soluble calcium salt solution and soluble phosphate solution were added to the above-mentioned mixed solution respectively, continuously stirred for 2 h, and the mass ratio of theoretically forming HAP was 1%;

[0059] 5) Add the cross-linking agent EDC / NHS, the mass ratio of EDC is 0.02%, the mass ratio of EDC and NHS is 2:1, and cross-link at room temperature for 5 hours; the...

Embodiment 2

[0062] 1) Prepare 2mol / L soluble calcium salt solution and 1.2mol / L soluble phosphate solution; the contents of chitosan, chondroitin sulfate and hyaluronic acid are respectively 1%, 0.1% and 0.05% by mass percentage;

[0063] 2) dissolving the chitosan in the ratio in 1% acetic acid solution, and dissolving the chondroitin sulfate / hyaluronic acid in the ratio in deionized water;

[0064] 3) Slowly add the chondroitin sulfate / hyaluronic acid mixture into the chitosan acetic acid solution, stir to make it fully mixed;

[0065] 4) According to n(Ca 2+ ):n(PO 4 3- )=1.67:1 ratio, a certain amount of soluble calcium salt solution and soluble phosphate solution were added to the above-mentioned mixed solution respectively, continuously stirred for 1 h, and the mass ratio of theoretically forming HAP was 0.5%;

[0066] 5) Add the cross-linking agent EDC / NHS, the mass ratio of EDC is 0.02%, the mass ratio of EDC and NHS is 3:1, cross-link at room temperature for 4 hours; the final...

Embodiment 3

[0069] 1) Prepare 3mol / L soluble calcium salt solution and 0.6mol / L soluble phosphate solution; the contents of chitosan, chondroitin sulfate and hyaluronic acid are respectively 2%, 0.2% and 0.065% by mass percentage;

[0070] 2) dissolving the chitosan in the ratio in 2% acetic acid solution, and dissolving the chondroitin sulfate / hyaluronic acid in the ratio in deionized water;

[0071] 3) Slowly add the chondroitin sulfate / hyaluronic acid mixture into the chitosan acetic acid solution, stir to make it fully mixed;

[0072] 4) According to n(Ca 2+ ):n(PO 4 3- )=1.67:1 ratio, a certain amount of soluble calcium salt solution and soluble phosphate solution were added to the above-mentioned mixed solution respectively, continuously stirred for 1.5 h, and the mass ratio of theoretically forming HAP was 0.8%;

[0073] 5) Add the cross-linking agent EDC / NHS, the mass ratio of EDC is 0.04%, the mass ratio of EDC and NHS is 3:1, and cross-link at room temperature for 6 hours; th...

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Abstract

The invention discloses an inorganic / organic diphase nano composite bone tissue engineering scaffold and a preparation method thereof. The bone tissue engineering scaffold is prepared by adopting an in-situ bionic method and a nano self-assembly technology; chitosan, chondroitin sulfate and hyaluronic acid are used as organic media; a soluble calcium salt and a soluble phosphate are used as precursors of inorganic phase nano hydroxyapatite; and by simulating bio-mineralization and adopting a mode of controlling the growth of the inorganic phase through organic macromolecules, the inorganic / organic diphase nano composite bone tissue engineering scaffold, namely the nano hydroxyapatite / chitosan / chondroitin sulfate / hyaluronic acid bone tissue engineering scaffold, is prepared on the organic matrix through in-situ nucleation and crystallization. The preparation method is simple and mild in process conditions; and the prepared composite scaffold is similar to a natural bone on macro and micro, and is excellent in performance and convenient to form and process.

Description

technical field [0001] The invention belongs to the field of bone tissue engineering scaffold material manufacturing, and in particular relates to an inorganic / organic dual-phase nanocomposite bone tissue engineering scaffold and a preparation method thereof. Background technique [0002] Bone tissue defect caused by trauma, tumor, infection, pathology and other factors is one of the major clinical problems. The repair methods include: autologous bone graft repair, allogeneic, heterogeneous bone graft repair, and various artificial bone substitute materials such as metal alloys and high molecular polymers. However, the supply of autologous bone is limited, and there are risks of immune rejection and disease infection in allogeneic and xenogeneic bone; while biomedical materials such as metals and organic polymers, which have been widely used in the medical field for a long time, have completely different components from natural bone. As a bone substitute material or to fill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/20A61L27/12A61L27/02
Inventor 陈景帝余其凤张玉珏张惠张其清
Owner FUZHOU UNIV
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