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Microcrystal catechin and preparation method thereof

A technology of catechins and microcrystals, which is applied in the field of medicine, can solve the problems of unresolved tea polyphenols anti-oxidation, unstable quality of tea polyphenols, and poor functionality, so as to achieve excellent anti-oxidation performance and prevent atherosclerosis Strong effect of hardening and quality stability

Active Publication Date: 2013-01-16
王孝仓
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0024] The current tea polyphenols are unstable in quality, easy to oxidize, have a short shelf life, poor functionality, and problems in the process. Tea polyphenols, including catechins, contain many phenolic hydroxyl groups, are extremely active, and are easily oxidized into quinone compounds. At present, tea polyphenols have not solved their own anti-oxidation problem, and the shelf life is too short to be unilaterally prepared, and only stay in the stage of animal experiments, pharmacodynamic mechanism studies or in vitro simulation experiments.

Method used

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  • Microcrystal catechin and preparation method thereof
  • Microcrystal catechin and preparation method thereof
  • Microcrystal catechin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: Preparation of the microcrystalline catechin of the present invention

[0056] The specific process is as follows:

[0057] (1) Steaming: Put green tea and deionized water into a pot to heat and cook, so that the effective substances in the tea can be dissolved in water. The ratio of deionized water to dry green tea is (2.5-3): 1, and the heating temperature is 85-100 ℃, the heating time is 15-30 minutes, then filter the tea soup into the container.

[0058](2) Membrane technology: The filtered tea soup is treated with an ultrafiltration membrane to remove impurities in the tea soup and concentrate it to 40-50% strong tea soup.

[0059] (3) Ethyl acetate extraction: pump the strong tea soup obtained in step (2) into the extraction tower, add ethyl acetate to extract catechins, the weight ratio of ethyl acetate to strong tea soup is 2:1, and the extraction time is 50- 80 minutes.

[0060] (4) Recovery of ethyl acetate: pump the two liquids obtained in st...

Embodiment 2

[0063] Embodiment 2: X-ray powder diffraction spectroscopic analysis of the microcrystalline catechin of the present invention

[0064] The microcrystalline catechin prepared in Example 1 was subjected to X-ray powder diffraction, using Cu-Kα radiation, and sent to the Central Laboratory of Nankai University for X-ray diffraction analysis.

[0065] Instrument: Rigaku D / MAX-2500 diffractometer

[0066] Target: Cu-Kα radiation (λ=1.5405A), 2θ=3°~50°

[0067] Step angle: 0.04°

[0068] Pipe pressure: 40KV

[0069] Pipe flow: 100mA

[0070] Scanning speed: 8° / min

[0071] Filter: graphite monochromator

[0072] Counter: Blink Counter

[0073] The microcrystalline catechin x-ray powder diffraction pattern prepared by embodiment 1 is as follows figure 1 As shown, using Cu-Kα radiation, the X-ray powder diffraction spectral characteristics expressed in 2 θ angles are shown in the table below.

[0074] 2θ

[0075] The sample (TPI) was analyzed to be microcrystalline....

Embodiment 3

[0076] Example 3: Microcrystalline catechin component analysis

[0077] The content of the microcrystalline catechin of the present invention, composition determination are as follows:

[0078] Measurement items

standard

The measurement results

test results

Polyphenol content%

≥40

97.1

Standards compliant

Loss on drying%

≤5.0

4.4

Standards compliant

Ignition residue %

≤0.5

0.13

Standards compliant

Arsenic (calculated as AS)%

≤0.0002

0.00003

Standards compliant

Lead (calculated as Pb)%

≤0.0004

＜0.00004

Standards compliant

[0079] Microcrystalline catechins (tea polyphenols) also have a strong hydrogen-supplying capacity, utilizing the ability of catechins to scavenge free radicals up to 98% in the xanthine-xanthine oxidase system. The Institute of Molecular Medicine of Zhejiang University ...

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Abstract

The invention relates to the field of medicine and discloses a microcrystal catechin and a preparation method thereof. The microcrystal catechin is characterized by spectral signatures of x-ray powder diffraction which uses Cu-K alpha radiation and is expressed at the angle 2 theta. The preparation method comprises the five procedures of steaming, membrane technology, organic solvent extraction, solvent recovery, and concentrating and drying. The microcrystal catechin has stable quality, long shelf life, good effects in treating hyperlipaemia and cardiovascular and cerebrovascular diseases, excellent oxidation resisting property and the excellent functions of diminishing inflammations, killing bacteria, clearing radicals, resisting tumors, resisting mutations, lowering blood fat and preventing atherosis. The microcrystal catechin is a valuable nature drug and has a wide clinic application prospect.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a microcrystalline catechin and a preparation method thereof. Background technique [0002] Tea (Tea, camellia sinenxis) has a history of 4,700 years in my country. The tea culture has a long history in China. my country produces 700,000 tons of tea every year and sells them all over the world. Tea trees are now planted in more than 30 countries. Studies have shown that tea is not only refreshing and brain-invigorating, but also has anti-inflammatory, anti-bacteria, elimination of free radicals, anti-cancer and anti-mutation, lower blood pressure, blood sugar, anti-oxidation, anti-aging and other effects. Tea is rich in the biological activity of polyphenolic compounds, that is, tea polyphenols, which account for about 20-25% of the dry weight of tea. According to their chemical structure, they can be divided into 4 categories: [0003] 1. Catechins (catcnins), which belong to flavanols,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61P3/06A61P9/00A61P31/04A61P35/00A61P39/06A61P9/10A61P31/10A61P31/12A61P37/08A23D9/06A23L3/3544A23G9/36
Inventor 王孝仓
Owner 王孝仓
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