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Cynanchum chinense extract and preparation method thereof

A technology of goose down vine and extract, applied in the field of biomedicine, can solve the problems of few research reports and the like

Active Publication Date: 2013-01-02
SHANGHAI GREEN VALLEY PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been reported in the literature that the water extract of the whole herb of the velvet vine has anticonvulsant, central inhibitory, sedative and hypnotic effects on experimental animals, and it contains proteases, flavonoids and other components (Ma Yan, Zhou Fengqin. Folk medicine velvet vine chemical composition, pharmacology and clinical application Research overview. Chinese Journal of Traditional Chinese Medicine, 2010, 28: 289-291.), but there are fewer overall research reports

Method used

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  • Cynanchum chinense extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the extraction of goose down rattan

[0014] The aerial part of the velvet vine was collected from the suburbs of Shihezi, Xinjiang Autonomous Region in 2010 (batch CC20100901), and was identified as the stem and leaf of the velvet vine (Cynanchum chinense R.Br.). Take 3 kg of powder of the aerial part of the velvet vine, reflux extraction with 8 times the amount of 70% ethanol solution (2h×3 times), combine the extracts, concentrate under reduced pressure to obtain liquid extract extract A (3L), and set aside. According to HPLC analysis, the contents of wheat flavin-7-O-β-D-glucuronide and kaempferol-3-O-β-D-glucopyranoside were 3.12 and 0.061 mg / g respectively.

Embodiment 2

[0015] Embodiment 2: the preparation of velvet vine extract

[0016] Take 2L of the extract in Example 1, disperse it with 6L of water, adjust the pH to 7-9, filter, and put the filtrate on an AB-8 macroporous resin column (1000×100mm i.d., column volume 2.0L / BV), and then water and 10%, 95 % ethanol (each 8 L) was eluted, and the 95% ethanol eluent was concentrated under reduced pressure and dried to obtain the extract B of the velvet vine, a total of 106 g. According to HPLC analysis, the contents of wheat flavin-7-O-β-D-glucuronide and kaempferol-3-O-β-D-glucopyranoside were 50.32 and 1.161 mg / g respectively.

[0017] Content determination: using Agilent 1260 Infinity Series high performance liquid chromatography; DAD detector, detection wavelength 360nm; chromatographic column is Cosmosil AR-II-C 18 (4.6mm×250mm, 5μm); flow rate 1mL min -1 , column temperature 35°C. The mobile phase is 0.6% phosphoric acid solution (A)-acetonitrile (B), gradient elution (0~12min, 18%B; ...

Embodiment 3

[0019] Embodiment 3: the preparation of velvet vine extract

[0020] Take 0.8L of the extract in Example 1, disperse it with 2.2L of water, adjust the pH to 7-9, filter, put the filtrate on a polyamide column (600×40mm i.d., column volume 400mL / BV), and successively water and 20%, 95% ethanol ( Each 8 L) was eluted, and the 95% ethanol eluate was concentrated under reduced pressure and dried to obtain the velvet vine extract C, a total of 32 g. According to HPLC analysis, the contents of wheat flavin-7-O-β-D-glucuronide and kaempferol-3-O-β-D-glucopyranoside were 67.36 and 1.32 mg / g respectively.

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Abstract

The invention discloses a cynanchum chinense extract and a preparation method thereof. The preparation method comprises the following steps: (1) selecting overground part stem leaves of calamus plant medicine, extracting with water or an ethanol-water mixed solvent, and concentrating and filtering an extracting solution; and (2) absorbing the extracting solution with macroporous resin or polyamide, carrying out gradient elution with the ethanol-water mixed solvent, collecting eluted parts of corresponding target products, and concentrating and drying the eluted parts to obtain the cynanchum chinense extract. The cynanchum chinense extract comprises more than 30mg / g of Tricin-7-O-Beta-D-glucuronide, and at least two components of tricin, kaempferol-3-O-Beta-D- glucopyranoside, apigenin and kaempferol. Plant resources of the preparation method disclosed by the invention can be recycled. The preparation method disclosed by the invention has the advantages of economic and reasonable process route, high efficiency, low energy consumption, convenience in recovering and recycling the solvent, and easiness in realizing the industrial production. The cynanchum chinense extract has the anti-inflammatory action, and can be applied in anti-inflammatory medicine.

Description

technical field [0001] The invention relates to an extract of velvet vine, and also relates to a preparation method of the extract and its application in treating inflammation-related diseases, belonging to the field of biomedicine. technical background [0002] Cynanchum chinense (Cynanchum chinense R.Br.) is a plant of the genus Cynanchum chinense R.Br., which grows in bushes, roadsides, riversides or field ridges. Its roots and fresh stems are used for folk medicine. The roots can dispel wind, detoxify, and strengthen Stomach pain relief and stem milk external application to treat warts have different purposes. It has been reported in the literature that the water extract of the whole herb of the velvet vine has anticonvulsant, central inhibitory, sedative and hypnotic effects on experimental animals, and it contains proteases, flavonoids and other components (Ma Yan, Zhou Fengqin. Folk medicine velvet vine chemical composition, pharmacology and clinical application Rese...

Claims

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Application Information

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IPC IPC(8): A61K36/27A61P29/00
Inventor 刘江云陈虹冯建勇杨世林
Owner SHANGHAI GREEN VALLEY PHARMA
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