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Combined Newcastle disease and infectious bronchitis inactivated vaccine and method for preparing same

A technology of inactivated vaccines and Newcastle disease, which is applied in the direction of medical formulas, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve problems such as difficulties, achieve simple and fast operation, save man-hours, and benefit absorption effect

Inactive Publication Date: 2012-10-10
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially for inactivated vaccines, it is very difficult to prepare a vaccine that can induce sufficient immune protection over a long period of time and has good reproducibility

Method used

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  • Combined Newcastle disease and infectious bronchitis inactivated vaccine and method for preparing same
  • Combined Newcastle disease and infectious bronchitis inactivated vaccine and method for preparing same
  • Combined Newcastle disease and infectious bronchitis inactivated vaccine and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] New, branch dual inactivated vaccine prepared by the present invention of embodiment 1

[0025] The LaSota strain of Newcastle disease virus is used to manufacture this product, and the virulent Beijing strain of chicken Newcastle disease virus (CVCC AV1611) is preferably used for efficacy testing. The infectious bronchitis virus strain used for seedling production and effect test is M41 strain.

[0026] Dilute the Newcastle disease virus seed LaSota strain virus seed into 10 4 EID 50 , the infectious bronchitis strain M41 was diluted to 10 6 EID 50 , after dilution, mix the two virus solutions at a ratio of 1:1. Inoculate 9-11-day-old susceptible chicken embryos with mixed virus seeds, inoculate 0.1ml of allantoic cavity in each embryo, seal the inoculation hole and stomata with wax, incubate at 37°C, discard dead embryos before 72 hours, and collect dead embryos 72-96 hours And 96h living embryo embryo fluid.

[0027] The embryo fluid was inactivated by 0.1% for...

Embodiment 2

[0028] The new and branch dual inactivated vaccine containing aluminum stearate produced by the conventional heteroembryon inoculation of embodiment 2

[0029] The LaSota strain of Newcastle disease virus is used to manufacture this product, and the virulent Beijing strain of chicken Newcastle disease virus (CVCC AV1611) is preferably used for efficacy testing. The infectious bronchitis virus strain used for seedling production and effect test is M41 strain.

[0030] 2. Inoculate 9-11-day-old susceptible chicken embryos with Newcastle disease virus species LaSota strain virus seeds and infectious bronchitis virus strain M41 respectively, and each embryo is inoculated with 0.1ml of the allantoic cavity, and the inoculation hole and stomata are sealed with wax. Incubate at 37°C, discard dead embryos before 72 hours, collect dead embryos at 72-96 hours and live embryos at 96 hours.

[0031]3. The harvested embryo fluid was inactivated by 0.1% formaldehyde at 37°C for 20 hours, a...

Embodiment 3

[0032] The new, branch dual inactivated vaccine character comparison that embodiment 3 two kinds of methods produce

[0033] 1. Appearance: Both are milky white homogeneous emulsions.

[0034] 2. Dosage form: Both are water-in-oil type. Drop a drop of oil emulsion on clean water, observe its shape, whether it disperses, and the time it disperses. The results showed that both were regular circular in shape and non-diffusion.

[0035] 3. Viscosity: Use a 1ml straw (inner diameter of the upper mouth is 2.7mm, and inner diameter of the lower mouth is 1.2mm), absorb 1ml of vaccine at about 25°C, let it flow out vertically and naturally, and record the time required for the outflow of 0.4ml. The results showed that the three outflow times of the two were all within 5s, which met the requirements of the regulations, and the viscosity of the vaccine prepared by the method of the present invention was lower, all within 3s.

[0036] 4. Stability:

[0037] (1) Take 10ml of the vaccin...

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Abstract

The invention discloses a method for producing a combined oil emulsion vaccine, and specifically relates a method for producing a combined Newcastle disease and infectious bronchitis inactivated vaccine, comprising the following steps of: carrying out homeomorphous inoculation, regulating the concentrations and proportions of Newcastle disease and infectious bronchitis viruses to achieve rational collocation, and regulating the components of an adjuvant in the vaccine so as to avoid aluminium stearate. The vaccine is capable of causing simultaneous production of Newcastle disease and infectious bronchitis antibodies, reducing disturbance, free of aluminium stearate, and capable of keeping high antibody production capability in a long time; and besides, the problems of stress reaction of animals and vaccine residue caused by vaccine injection can be solved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a dual inactivated vaccine for Newcastle disease and infectious bronchitis and a preparation method thereof. Background technique [0002] Newcastle disease virus (Newcastledisease virus, NDV) is also known as Asian fowl plague virus, pseudo fowl plague virus or avian pneumoencephalitis virus. Its position in virus taxonomy belongs to the family Paramyxoviridae and a species in the genus Paramyxovirus. The virus mainly harms chickens, guinea fowls and turkeys, and spreads rapidly among the infested flocks, and the virulent strain can destroy the whole flock of chickens. Attenuated strains only cause respiratory tract infection and decreased egg production in chickens, but they can recover quickly. Humans can develop conjunctivitis or lymphadenitis due to contact with sick birds and live virus vaccines, but recover quickly. Newcastle disease v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/215A61K39/17A61K47/44A61K47/24A61P31/14
Inventor 张许科孙进忠乔荣岑
Owner PU LIKE BIO ENG
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