Preparation method for liquefied strain of edible mushroom

A technology for edible fungi and strains, which is applied in the fields of botanical equipment and methods, applications, and gardening, and achieves the effects of simple inoculation, short production cycle and cost reduction.

Inactive Publication Date: 2012-10-10
浙江常山荣昇食用菌有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing edible fungus liquefied strains aimed at the defects in the existing edible fungus strain production methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of liquefied strains of Pleurotus geniferus:

[0022] step 1:

[0023] Purification and cultivation of solid strains of Pleurotus edodes: select solid medium components and the weight ratio is: cottonseed hulls: sawdust: wheat bran: corn flour: gypsum: lime: = 42: 40: 10: 5: 2: 1, add water Finally, the water content reaches 58% to 62%, mix well and bottle (750ml / bottle), sterilize under 0.15Mpa pressure for 2 hours to 2.5 hours, cool in a clean cooling room to below 28°C, and store under sterile conditions , connect the mother species of Pleurotus osmanthus to the solid medium, and one 20mm×200mm mother seed is connected to 4 to 6 bottles of solid medium, and then moved into the cultivation room at 23°C to 25°C, keeping the air humidity at 60% to 75%. , after 30 to 35 days of ventilating and avoiding light to grow bacteria, cultivate the solid strains of pear mushrooms;

[0024] Step 2: Preparation of sterile water: use steam sterilization to prepare ster...

Embodiment 2

[0028] Preparation of Flammulina velutipes liquefaction strain:

[0029] step 1:

[0030] Purification and cultivation of Flammulina velutipes solid strain: select solid medium components and the weight ratio is: cottonseed hull: sawdust: wheat bran: corn flour: rice bran: gypsum powder: lime: =50: 18: 15: 10: 5: 1.5: 0.5, the water content reaches 58%~62% after adding water, mix well and bottle (750ml / bottle), sterilize under 0.15Mpa pressure for 2 hours~2.5 hours, cool in a clean cooling room to below 28°C, Under sterile conditions, connect the female species of Flammulina velutipes to the solid medium, and connect 4 to 6 bottles of solid medium to one 20mm×200mm female species, and then move it into a sterile room at 21°C to 23°C to keep the air humidity After 60% to 75%, ventilated and protected from light for 35 to 40 days, cultivate the solid strain of Flammulina velutipes;

[0031] Step 2:

[0032] Preparation of sterile water: Prepare sterile water by directly boili...

Embodiment 3

[0038] Preparation of seafood mushroom liquefaction strains:

[0039] Step 1: Purification and cultivation of seafood mushroom solid strains: select solid medium components and the weight ratio is: cottonseed hulls: sawdust: wheat bran: corn flour: rice bran: gypsum powder = 50: 15: 15: 10: 9: 1 After adding water, the water content is 58% to 62%. After mixing evenly, bottle it (750ml / bottle), sterilize it under 0.15Mpa pressure for 2 hours to 2.5 hours, and cool it in a clean cooling room to below 28°C. Under the condition of bacteria, connect the mother species of seafood mushrooms to the solid medium, and connect 4 to 6 bottles of solid medium to one 20mm×200mm mother species, and then move it into a sterile room at 23℃~25℃ for 35 days~ After 40 days, cultivate the solid strain of seafood mushroom;

[0040]Step 2: Preparation of sterile water: directly prepare sterile water using a water filter;

[0041] Step 3: Liquefaction of seafood mushroom solid strain. Under asepti...

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Abstract

The invention discloses a preparation method for liquefied strains of edible mushrooms. The preparation method comprises four steps of purification culture of solid strains, sterile water preparation, solid strain liquefaction and liquefied strain inoculation. Under an aseptic condition, the solid strains are added into a crushing machine and are crushed into paste strains, then the sterile water is added for diluting the paste strains to obtain the liquefied strains, and finally the liquefied strains are poured into culture bags to complete the inoculation by an appliance. The preparation method for liquefied strains of edible mushrooms has the advantages of simplified process, short production period, simplicity and convenience in inoculation, rapid spore germination, uniform cell age, early fruiting, good quality, high yield, high efficiency, small pollution risk, small investment and operating cost, good economic benefit and the like.

Description

technical field [0001] The invention belongs to a method for cultivating edible fungus strains, in particular to a preparation method for liquefied edible fungus strains. Background technique [0002] The production of edible fungus strains is a key link in the cultivation of edible fungi. To achieve high-quality and high-efficiency edible fungus cultivation, the production of high-quality, high-efficiency, and low-cost strains must first be achieved. The production of existing edible fungus strains mainly includes two production methods: solid strains and liquid strains. The production process of solid strains is as follows: mother species→original species→cultivated species→bacteria bag (or cultivation bottle) , that is, under aseptic conditions, use a 20mm×200mm parent seed to connect 4 to 6 bottles (750ml / bottle) of solid medium that has been thoroughly sterilized, and culture it at an appropriate temperature to become the original seed; Then use 1 bottle of the origina...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 周国才黄良水
Owner 浙江常山荣昇食用菌有限公司
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