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Preparation method for phytosterol acetate formed by catalytic synthesis through enzymic method

A technology of phytosterol acetate and phytosterol, which is applied in the field of preparation of phytosterol acetate catalyzed by an enzymatic method, can solve problems such as safety hazards of acetic anhydride, safety of chemical synthesis methods, and environmental pollution, and achieve production The effect of short process flow, convenient industrial production and high esterification rate

Active Publication Date: 2013-10-30
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the phytosterol small molecule esters synthesized in China are mainly phytosterol acetate. In the article "Study on the Synthesis of Phytosterol Acetate", it is reported that the chemical catalyst is used as the reaction medium, and the phytosterol and acetic anhydride are used for the esterification synthesis reaction. To obtain phytosterol acetate, as we all know, acetic anhydride has a relatively large safety hazard, and the chemical synthesis method has also been questioned by people in terms of safety and environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Add phytosterol and pyruvic acid with a molar ratio of 1:5 into a 150mL triangular flask, add 10mL of dehydrated n-hexane, put it in a water bath constant temperature oscillator, 55°C, fully shake and mix for 30min, and wait for the reaction Add 5%wt lipase (based on the mass of pyruvic acid) as a catalyst, 40mg / mL (the amount of molecular sieve added in the reaction solvent per unit volume is 40mg) molecular sieve as a dehydrating agent, and react under stirring conditions at 55°C Ends in 18 hours. After the reaction solution is cooled, filter the reaction solution with suction to separate the enzyme, molecular sieve and a small amount of unreacted sterols. If the filtrate still has a small amount of turbidity, it can be filtered again. According to the fact that phytosterol esters have a strong absorption peak at 640nm of visible light , Quantitative determination of the amount of phytosterol esters, the esterification rate was 93.01%.

Embodiment 2

[0025] Add phytosterol and pyruvic acid with a molar ratio of 1:5 into a 150mL triangular flask, add 10mL of dehydrated n-hexane, put it in a water bath constant temperature oscillator, 55°C, fully shake and mix for 30min, and wait for the reaction The substance was completely dissolved, and 6% lipase (based on the amount of pyruvate) was added as a catalyst, 40 mg / mL molecular sieve was used as a dehydrating agent, and the reaction was completed after 16 hours at 55°C under stirring conditions. After the reaction solution is cooled, filter the reaction solution with suction to separate the enzyme, molecular sieve and a small amount of unreacted sterols. If the filtrate still has a small amount of turbidity, it can be filtered again. According to the fact that phytosterol esters have a strong absorption peak at 640nm of visible light , Quantitative determination of the amount of phytosterol esters, the esterification rate was 91.25%.

Embodiment 3

[0027] Add phytosterol and pyruvic acid with a molar ratio of 1:4 into a 150mL triangular flask, add 10mL of dehydrated n-hexane, put it in a water bath constant temperature oscillator, 55°C, fully shake and mix for 30min, and wait for the reaction The substance was completely dissolved, and 5% lipase (based on the amount of pyruvate) was added as a catalyst, 40 mg / mL molecular sieve was used as a dehydrating agent, and the reaction was completed after 20 hours at 55°C under stirring conditions. After the reaction solution is cooled, filter the reaction solution with suction to separate the enzyme, molecular sieve and a small amount of unreacted sterols. If the filtrate still has a small amount of turbidity, it can be filtered again. According to the fact that phytosterol esters have a strong absorption peak at 640nm of visible light , Quantitative determination of the amount of phytosterol esters, the esterification rate was 87.90%.

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Abstract

The invention relates to a preparation method for phytosterol acetate formed by catalytic synthesis through an enzymic method. The preparation method comprises the following steps: dissolving pyruvic acid and phytosterin as reaction substrates into dehydrated organic solvent; adding immobilized lipase and a dehydrating agent; and carrying out an oscillating reaction in a water bath of 40-60DEG C for 16-20 hours to obtain the phytosterol acetate, wherein the esterification rate is high as high as 93.01 percent. According to the preparation method disclosed by the invention, the phytosterol acetate is prepared from the pyruvic acid and the phytosterin as raw materials. The preparation method has the advantages of requirement on simple equipment, environment friendliness, safety and higher esterification rate.

Description

technical field [0001] The invention relates to the technical field of preparation of phytosterol esters, in particular to a preparation method for enzymatically catalyzing the synthesis of phytosterol acetate. Background technique [0002] As a class of natural active substances, phytosterols have a variety of physiological functions, and have been recognized as functional substances that can lower cholesterol, prevent prostate diseases, fight cancer, fight inflammation, and regulate physiological hormone levels. However, the low water and oil solubility of phytosterols limits its possibility to be directly developed into a healthy food base, while synthetic phytosterol esters (PE) not only improve its oil solubility, but also maintain its original biological activity . [0003] The application number is 200810059243.7. The Chinese patent application discloses that calcium oxide and magnesium oxide are used as catalysts to synthesize phytosterol esters at relatively high ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/00
Inventor 刘国琴李萍李琳巩凡
Owner SOUTH CHINA UNIV OF TECH
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