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Deer spleen extract preparation and preparation method thereof

A technology of deer spleen extract and deer spleen, which is applied in the field of deer spleen extract preparation and preparation, can solve the problems of waste of deer spleen resources and achieve the effect of simple operation process

Active Publication Date: 2012-09-19
融致丰生制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Deer products are widely recognized as medicinal resources in my country. Now there are deer-related products such as antler and deer fetus on the market, as well as spleen-related products, such as beef spleen and pig spleen, but deer spleen is abandoned. If it is not used, it will cause a serious waste of deer spleen resources. The research on the preparation of deer spleen extract can improve the utilization of deer spleen and improve the comprehensive benefits of deer resources.

Method used

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  • Deer spleen extract preparation and preparation method thereof
  • Deer spleen extract preparation and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Take 200 g deer spleen, wash it with deionization, remove the blood, remove the fascia, cut it into pieces, add 5 times of deionized water, put it into a colloid mill and grind it at 3000 rpm for 10 minutes. The pH of the crushed suspension was adjusted to 2.4, and then 500 U / g of pepsin was added for hydrolysis at 37°C for 4 h. Then the hydrolyzed suspension was centrifuged at 3000 rpm for 10 min, the supernatant was collected, concentrated by rotary evaporation, and spray-dried to obtain dry powder of deer spleen extract.

Embodiment 2

[0045] Take 200 g deer spleen, wash it with deionization, remove the blood, remove the fascia, cut it into pieces, add 5 times of normal saline, put it into a colloid mill and grind it at 3000 rpm for 10 minutes. The pH of the crushed suspension was adjusted to 8.5, and then 756 U / g of trypsin was added for hydrolysis at 37°C for 4 h. Then the hydrolyzed suspension was centrifuged at 5000 rpm for 10 min, the supernatant was collected, concentrated by rotary evaporation, and spray-dried to obtain dry powder of the deer spleen extract.

Embodiment 3

[0047] Take 200 g deer spleen, wash it with deionization, remove the blood, remove the fascia, cut it into pieces, add 5 times of deionized water, put it into a colloid mill and grind it at 3000 rpm for 10 minutes. The pH of the crushed suspension was adjusted to 7.0, and then 1200 U / g of neutral protease was added for hydrolysis at 37°C for 4 h. Then the hydrolyzed suspension was centrifuged at 5000 rpm for 10 min, the supernatant was collected, centrifuged with a 3KD ultrafiltration tube, and the polypeptide content of less than 10KD in the supernatant was measured, the rest of the supernatant was concentrated by rotary evaporation, and spray-dried Spleen extract dry powder.

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Abstract

The invention provides a deer spleen extract preparation and a preparation method thereof. The preparation method comprises the following steps of: adding water or normal saline or buffer solution into deer spleen, mashing with a colloid mill, performing enzymolysis, centrifuging, taking supernate, concentrating, and performing spray drying to obtain dry powder, wherein the dry powder can be mixed with other one or more Chinese medicaments to prepare a compound preparation, and the dry powder is prepared by the same method; and adding accessories acceptable in health-care food into the dry powder, and thus obtaining the oral preparation facilitating oral administration. The preparation has health-care functions of assisting in improving human immunity and the like; and the waste deer spleen is changed into valuable, so that the utilization rate of the deer spleen is improved.

Description

technical field [0001] The invention provides a deer spleen extract preparation and a preparation method, and also discloses its application in improving immunity, and belongs to the field of health-care and nutritious food. Background technique [0002] Deer spleen is the spleen of sika deer or red deer in the deer family. The spleen has the functions of promoting digestion, improving spleen and stomach functions, and improving human immunity. Deer products are widely recognized as medicinal resources in my country. Now there are deer-related products such as antler and deer fetus on the market, as well as spleen-related products, such as beef spleen and pig spleen, but deer spleen is abandoned. If it is not used, it will cause a serious waste of deer spleen resources. The research on deer spleen extract preparation can improve the utilization of deer spleen and improve the comprehensive benefits of deer spleen resources. [0003] At present, patents related to spleen are m...

Claims

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Application Information

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IPC IPC(8): A23L1/29A61K35/28A61P37/04A23L33/00A61K35/26
Inventor 滕利荣宋静静程瑛琨逯家辉孟庆繁郭璇刘艳闫国栋周毓麟
Owner 融致丰生制药有限公司
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