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Preparation method and application of metal surface-etched nanoporous array

A technology of nanohole array and surface etching, which is applied in chemical instruments and methods, optical waveguide coupling, crystal growth, etc., can solve problems affecting cell biological behavior, and is beneficial to adhesion and proliferation, and the manufacturing process is simple The effect of convenience and easy operation

Inactive Publication Date: 2012-07-11
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although TiO was prepared by anodic oxidation on the surface of titanium 2 There have been many research reports on the method of nanotubes, but the application of electrochemical anodic oxidation combined with chemical corrosion technology to prepare TiO with stable mechanical properties and specific dimensions on the titanium surface 2 Nanopore array structure, and related research on the influence of this type of material on cell biological behavior, has not been reported at home and abroad

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  • Preparation method and application of metal surface-etched nanoporous array
  • Preparation method and application of metal surface-etched nanoporous array
  • Preparation method and application of metal surface-etched nanoporous array

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of titanium dioxide nanopores

[0051] Main instruments and reagents:

[0052] Titanium flakes with a purity of 99% (Alfa Aesar Company, China), ammonium fluoride (analytical grade, Sinopharm Chemical Reagent Co., Ltd., China), DMEM medium (Gibco, USA), fetal bovine serum (FBS, Gibco, USA) ), trypsin dry powder (activity 1:250, Sigma, USA), EDTA (Huamei Bioengineering Company, China), programmable DC power supply (IT6834, Suzhou Outest Electronics Co., Ltd., China), (thermostatic magnetic stirrer (94-2, Shanghai Meiyingpu Instrument Manufacturing Co., Ltd., China), cryogenic constant temperature bath (DC-0506, Shanghai Hengping Instrument Factory, China), platinum electrode (Tianjin Aida Hengsheng Technology Development Co., Ltd., China) , laser scanning confocal microscope (Leica TCS SP2, Germany), field emission scanning electron microscope (Fei Sirion 200, Philip, Netherlands), atomic force microscope (BioScope, Veeco Company, USA), fluoresce...

Embodiment 2

[0069] Example 2 TiO on titanium surface 2 Characterization of nanopores

[0070] The effect of different nano-modifications on the titanium surface on the biological behavior of cells is determined by the interaction between the biomaterials on the titanium surface and the cells. The interaction between the cells and the titanium implant material only occurs at the interface between the tissue and the titanium, so the characteristics of the titanium surface are in the important role in this interaction. In this example, 99% titanium sheet was used as the control group, and TiO was prepared by electrochemical anodic oxidation on the surface. 2 Nanoporous titanium sheet is the treatment group, using scanning electron microscope, atomic force microscope, X-ray diffractometer, material surface contact angle measuring instrument 2 Characterization of nanopores.

[0071] 1. Surface SEM images of the treatment group and the control group

[0072] See image 3 , the scanni...

Embodiment 3

[0081] Example 3 Titanium Surface TiO 2 Effects of nanopores on the adhesion and proliferation of bone marrow stromal cells

[0082] 1. Materials and methods

[0083] 1. Isolation and culture of porcine bone marrow stromal cells (BMSCs)

[0084] Choose 3-month-old healthy hybrid pigs, male or female. Ketamine intramuscular injection induction and intravenous injection of pentobarbital sodium compound anesthesia, about 4mL of bone marrow blood was drawn from the ilium, placed in serum-free DMEM culture medium, and mixed well. 2000r / min, centrifuge for 20min. Discard the supernatant and floating fat, and keep the interface layer and blood cell layer. Add DMEM culture solution containing 100mL / L fetal bovine serum, inoculate on a petri dish, and incubate at 37°C, 5% CO 2 Primary culture in a saturated humidity incubator. After 5 days, the medium was changed in full volume, and the unattached blood cells were replaced. After the cells reached about 80% confluence, the...

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Abstract

The invention relates to application of pure titanium and titanium alloy (hereinafter called titanium) surface-etched TiO2 nano-porous array appearance in preparing human metal implant which can be a dental implant, a bone implant, a fixing plate and a fixing nail, wherein the appearance is to prepare a TiO2 nano-porous array structure on the titanium surface. The invention also provides an optimal preparation method of the appearance. The preparation method has the advantages that: the preparation can be performed at normal temperature and normal pressure; the prepared TiO2 nano-porous array is regular; and the method is relatively simple in process, convenient for operation, low in cost and short in process flow. The prepared titanium surface nano-porous array structure has selective effect to different types of cells, so that the success rate of implant can be greatly increased; the nano-porous array structure can inhibit fibroblast from adhering or multiplying on the surface of the structure, so that the success rate of bone and dental implant operation and bone internal fixation can be greatly improved; and therefore, the preparation method is a bone implant material and bone fixation material surface appearance modification method with clinical application prospect.

Description

technical field [0001] The invention relates to a novel implant material and its preparation method and application, in particular, a titanium surface TiO 2 The application of the nanohole array structure and its preparation method. Background technique [0002] Biomedical materials refer to non-living materials that are used for medical purposes and are used to contact living tissues and achieve certain functions (International Organization for Standardization ISO, 1987.10). In-depth study of the interaction between implanted materials and the human body and the resulting material and host responses is the prerequisite and necessary condition for the development of ideal biomedical materials. The interaction between the material surface and the receiving tissue cells is one of the key factors for the clinical application effect of bone implant materials and the success of treatment. The surface properties of materials affect the adhesion and growth of tissue cells, and wi...

Claims

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Application Information

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IPC IPC(8): A61L27/30A61L27/06A61L31/08A61L31/02C25D11/26C23F1/38C30B30/02C30B29/16C30B29/62B81B7/04
Inventor 陈万涛黄其煜孙强陈姗姗严明徐骎张梅
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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