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Alkaloid extract of trembling poplar as well as extraction method and application thereof

A technology of Liriodendron tulipifera and extract, which is applied in the field of alkaloid extract of Liriodendron tulipifera and its extraction method and anti-tumor application, can solve the problems that the medicinal value of hybrid trees has not been studied, and achieve good economic benefits and social benefits. High benefit, high product yield, good inhibitory effect

Active Publication Date: 2013-11-13
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the medicinal value of hybrid trees has not been studied, especially in the research of drugs for the treatment of tumors

Method used

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  • Alkaloid extract of trembling poplar as well as extraction method and application thereof
  • Alkaloid extract of trembling poplar as well as extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take the freshly collected Liriodendron tulipifera material, separate the leaves and bark, and immediately dry it in the air at 35°C. Grind the dry material to medium fine powder, about 40 mesh, and set aside.

[0017] Take 350g of Liriodendron tulipifera plant material, dry it at 35°C, and crush it into a fine powder of about 40 mesh; soak it in 10% ammonia water for 12 hours, filter to obtain the residue; after 1 hour, reflux it with 2 L of chloroform for 2 hours, filter it, concentrate it, and use %~2% hydrochloric acid was extracted 5 times, 100 mL / time, the hydrochloric acid extract was adjusted to pH > 8 with 10% ammonia water, then extracted with chloroform, 100 mL / time, a total of 3 times, filtered, combined, and evaporated to dryness to obtain Total Alkaloid Extract.

[0018] The method of extracting the leaves and bark of each Liriodendron genus is the same as above, and the weight of the extracts of different plant materials is shown in Table 1.

[0019] Ta...

Embodiment 2

[0033] Take the freshly collected Liriodendron tulipifera material, separate the leaves and bark, and immediately dry it in the air at 35°C. Grind the dry material to medium fine powder, about 40 mesh, and set aside. Take 350g of Liriodendron tulipifera plant material as a medium fine powder, soak it with 8% ammonia water for 24 hours, filter to obtain the residue; after 1 hour, reflux with 2 L of chloroform for 1 hour, filter, concentrate, and extract with 1%~2% hydrochloric acid3 Each time, 100 mL / time, the hydrochloric acid extract was adjusted to pH > 8 with 10% ammonia water, and then extracted with chloroform, 100 mL / time, a total of 3 times, filtered, combined, and evaporated to dryness to obtain the total alkaloid extract.

[0034] The method of extracting from leaves and bark of each Liriodendron genus is the same as above. The extract yields of different plant materials are shown in Table 3.

[0035] Table 3 Extract yield table of different plant materials

[0036...

Embodiment 3

[0042] MCF-7 cells were cultured using ATCC complete medium, including basic medium EMEM (Eagle's Minimum Essential Medium), balanced salt solution EBSS (Earles Balanced Salt Solution), non-essential amino acids, 2mM L-glutamine, 1 mM sodium pyruvate , 1.5mg / mL sodium bicarbonate solution, 0.01mg / mL bovine insulin, 10% fetal bovine serum, 25cm containing 100U / mL penicillin and 100 mg / mL streptomycin 2 Tissue culture flasks. Temperature controlled at 37°C, in the presence of 5% (v / v) CO 2 incubated in saturated water vapor.

[0043] Breast cancer MDA-MB-231 cells were cultured with ATCC complete medium, including L-15 and 2 mM L-glutamine, 1.5 mg / mL sodium bicarbonate, 10% fetal bovine serum, containing 100 U / mL penicillin and 100 mg / mL 25 cm mL of streptomycin 2 Tissue culture flasks, temperature controlled at 37°C, in 5% (v / v) CO 2 incubated in saturated water vapor.

[0044] The culture conditions of the other three human tumor cells were the same as the above two, exce...

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Abstract

The invention discloses an alkaloid extract of trembling poplar as well as an extraction method and application thereof. The extraction method of the alkaloid extract of trembling poplar comprises the following steps: baking a trembling poplar plant material at 35 DEG C, crushing to obtain fine powder with the granularity of about 40 meshes; infiltrating with 10% ammonia water for 12 hours, and filtering to obtain residues; and 1 hour later, refluxing by use of 2L of chloroform for 2 hours, filtering, concentrating, extracting five times, 100mL per time, by use of 1-2% hydrochloric acid, regulating the pH value of the hydrochloric acid extract to be more than 8 by use of 10% ammonia water, extracting with chloroform for 3 times, 100mL per time, filtering, combining, and evaporating to dryness, thus obtaining the alkaloid extract. The extract method is simple and convenient and the yield of alkaloid is high; the alkaloid extract of the trembling poplar has excellent capability of inhibiting growth, proliferation and transfer of tumor cells, has wide application in preparing a medicament for treating cancer cells, has certain practicality, and can generate good economical and social benefits.

Description

technical field [0001] The invention relates to a tree tissue extract, its extraction method and application, in particular to an alkaloid extract of Liriodendron tulipifera, its extraction method and its anti-tumor application. Background technique [0002] There are only two species of Liriodendron genus in the family Magnoliaceae, one is Liriodendron tulipifera, which is naturally distributed in the eastern United States, and the other is Liriodendron japonica, which is distributed in southern my country. Both species have historical records of pharmacy in the United States, China, and other countries around the world. Liriodendron tulipifolia, tulip tree or boxwood, is a tall tree, often reaching a hundred feet in height, endemic to the eastern United States (Keeler, 1902). The bark of Liriodendron tulipifera was used by the Indians as a tonic, stimulant, and antipyretic, and possibly in the treatment of intermittent fevers caused by malaria (Rafinesque et al., 1828). ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/57A61P35/00A61K127/00A61K129/00
Inventor 施季森陈金慧贾爱群魏继福
Owner NANJING FORESTRY UNIV
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