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Preparation method of lipid with structure of polyunsaturated fatty acids

A technology of unsaturated fatty acids and structural lipids, applied in the biological field

Active Publication Date: 2019-02-15
河北康睿达脂质有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More importantly, the synthesized structural lipids with different carbon chains can be mixed in different proportions to meet the nutritional needs of different groups of people, and provide theoretical basis and technical support for the large-scale preparation of structural lipids from different fatty acid sources in the future , has positive and practical guiding significance for the localization of foreign monopoly products, improving the resource utilization of oils, and extending the industrial chain of my country's oil industry, but it has no effect on the combination of structural lipids and other substances to form new nutrients for human use. Pay attention to improving the body's absorption of nutrients such as structural lipids

Method used

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  • Preparation method of lipid with structure of polyunsaturated fatty acids
  • Preparation method of lipid with structure of polyunsaturated fatty acids
  • Preparation method of lipid with structure of polyunsaturated fatty acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 The purifying borage oil (the weight content of r-linolenic acid is 20%) of the refined fish oil 29 weight parts of 55 weight parts, the ingot maple seed oil of 6 weight parts, the pine nut oil of 10 weight parts (Pinoline acid) and 6 parts by weight of immobilized lipase 435 were reacted in a closed container under a vacuum condition of 50 Pa, a reaction temperature of 55° C., and a reaction time of 20 hours to terminate the reaction. Entering the refining process, after the reaction product rises close to 120°C and 160°C dry steam is mixed with flash evaporation, the distillation realizes the deacidification, deodorization and passivation of enzyme activity, the flash vacuum is 100Pa, cooling, fine filtration of polyunsaturated fatty acid structured lipids.

Embodiment 2

[0047] Embodiment 2 with 3 parts by weight (the content of reduced fish oil EPA and DHA is 65%), the borage oil of 55 parts by weight (the r-linolenic acid weight content is 87%), the pine nut oil (Pinolar) of 15 parts by weight The weight content of acid is 17%) and 10 weight parts of RM, IM lipase in ultrasonic packed column reaction closed-loop 46 hours, obtain reaction product, then through vacuum 100-200Pa, steam temperature 190 ℃, flash evaporation, deodorization, Inactivate the enzyme activity, cool down to 60°C, add 0.2‰ antioxidant, mix and homogenize, and fill the finished product.

Embodiment 3

[0048] Embodiment 3 The polyene fish oil (the weight content of EPA and DHA is greater than 75%) of 15 weight parts, the evening primrose oil (the weight content of r-linolenic acid is 9%) of 77 weight parts, the pine nut oil of 8 weight parts (the weight content of pinolenic acid is 17%) and the 435 lipases of 8 weight parts, in the supersonic bed layer reactor closed-circuit circulation reaction 28 hours, terminate reaction and enter flashing procedure after fine filtration of product, before flashing, The oil product is first heated to 120°C, and then deodorized and inactivated by 180°C dry steam under a vacuum of 100-200Pa. The finished product is cooled to 60°C, and oil-soluble VE0.2‰ is added, mixed homogeneously, and the finished product can be packed.

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Abstract

The invention relates to a preparation method. The raw materials in the preparation method are at least refined or purified oil which comprises r-linolenic acid and oil which comprises timnodonic acidand docosahexenoic acid. The raw materials also comprise one or more of acer truncatum seed oil, malania oleifera oil, pine nut oil, coconut oil, palm oil and moringa oleifear seed oil. The preparation method comprises the steps: adding an ester exchange enzyme into the raw materials to react in a closed container; and carrying out distillation, deacidification, deodorization and modified enzymeactivation on the raw material after the ester exchange enzyme reaction to obtain the lipid. One or more fish oil EPA / DHA / DPA r-linolenic acid, C24: 1 nervonic acid, pinolenic acid and the like are combined to one oil molecule to form the lipid containing sn-1-3 site and sn-2 site fatty acids, and meanwhile, the prepared lipid can be combined with other nutritional substances to form novel nutritional food which is suitable for being eaten by pre-operative, rehabilitative and special crowds. The lipid also can be prepared into fructose biscuits, special food hard to swallow and chew and otherfood, so that the individual application direction is wider.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to enzyme modification technology, in particular to a method for preparing polyunsaturated fatty acid structured lipids. Background technique [0002] As we all know, structured lipids (Structured lipids, SLs) are modified natural lipids, directional addition of short carbon chain fatty acids, medium carbon chain fatty acids and long carbon chain unsaturated fatty acids, because of their special fatty acid composition and fatty acid content in triglycerides The specific position in the ester makes it have special physiological functions and nutritional value. In recent years, there has been a strong link between high fat and coronary heart disease, obesity, and certain cancers. Fat plays a unique and important role in various foods: providing energy, essential fatty acids and promoting the absorption of fat-soluble vitamins and calcium (Journal of Agricultural and Food Chemistry, 2012,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64A23L2/39A23L2/52A23L2/60A23L33/12A23L33/00A23G3/40A21D13/06A21D2/18A21D2/26A21D2/16
CPCA21D2/16A21D2/18A21D2/186A21D2/26A21D13/06A23G3/40A23L2/39A23L2/52A23L2/60A23L33/12A23L33/40C12P7/6472
Inventor 刘宪军来创业苑洪德王延荣刘宝华苑海鹏苑海祥
Owner 河北康睿达脂质有限公司
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