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Liquid fermentation method for producing paecilomyces cicadae mycelia and application of culture product

A Paecilomyces cicadae, liquid fermentation technology, applied in the field of microbial fermentation, can solve the problems of long cultivation period, less liquid fermentation, low yield, etc., and achieve the effect of stable product quality, stable quality and high yield

Active Publication Date: 2011-11-16
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the artificial cultivation methods of cicadae in the existing patents focus on solid fermentation, and few involve liquid fermentation
However, solid fermentation often has long cultivation period, low yield and easy pollution.

Method used

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  • Liquid fermentation method for producing paecilomyces cicadae mycelia and application of culture product
  • Liquid fermentation method for producing paecilomyces cicadae mycelia and application of culture product
  • Liquid fermentation method for producing paecilomyces cicadae mycelia and application of culture product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Preparation and cultivation of strains:

[0026] 1) Strain preparation: including preparation of slant seed and shake flask seed;

[0027] Wherein, the preparation of the slant seed comprises the following steps: transplanting the isolated and purified Paecilomyces cicadae strain into a PSA slant test tube, cultivating it at 24° C. for 7 days, and selecting a good-growing seed for future use;

[0028] The preparation of the shake flask seed includes the following steps: insert the slant seed into the potato sucrose culture solution, cultivate it for 5 days at 25°C and the oscillation frequency is 140r / min, and use it as a shake flask after a large number of mycelium balls appear in the Erlenmeyer flask kind of spare;

[0029] The PSA solid medium contains the following components (contained in every 1000ml medium): 200g of potatoes, 20g of sucrose, 20g of agar;

[0030] The potato sucrose culture solution contains the following components (contained in every 1000ml...

Embodiment 2

[0040] 1. Preparation and cultivation of strains: use Paecilomyces cicadae (Miq.) Samson] CGMCCNo.3453 for liquid fermentation

[0041] 1) Strain preparation: including preparation of slant seed and shake flask seed;

[0042] Wherein, the preparation of slant seeds includes the following steps: transplanting the isolated and purified strains into PSA slant test tubes, cultivating them at 24°C for 7 days, and selecting good-growing seeds to store for later use;

[0043] The preparation of the shake flask seed includes the following steps: insert the slant seed into the potato sucrose culture solution, cultivate it for 5 days at 25°C and the oscillation frequency is 140r / min, and use it as a shake flask after a large number of mycelium balls appear in the Erlenmeyer flask kind of spare;

[0044] The PSA solid medium contains the following components (contained in every 1000ml medium): 200g of potatoes, 20g of sucrose, 20g of agar;

[0045] The potato sucrose culture solution c...

Embodiment 3

[0119] Example 3 The influence of Paecilomyces cicadae mycelia water extract on the phagocytosis of peritoneal macrophages by Wright-giemsa staining

[0120] 1. Experimental materials and methods

[0121] 1.1 Sample: artificially cultivated Paecilomyces cicadae mycelium water extract, the mycelium prepared by the method described in Example 2 was obtained by conventional water extraction.

[0122] 1.2 Experimental animals and environment: 30 Kunming mice, half male and half male, weighing 19-22 grams, were provided by Shanghai Slack Experimental Animal Co., Ltd., fed with conventional feed. Production license number: SCXK (Shanghai) 2007-0005. Breeding room temperature 20-25 ℃, relative humidity: 40-70%. They were randomly divided into 3 groups according to sex, with 5 males and 5 males in each group.

[0123] 1.3 Experimental method: every rat first ip 5% normal saline chicken erythrocyte suspension 0.2ml is immunized, blank group ig equal volume distilled water, large and...

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Abstract

The invention belongs to the technical field of microbial fermentation, and in particular relates to a liquid fermentation method for producing paecilomyces cicadae mycelia and application of a culture product. The method comprises the following steps of: preparing a culture medium, preparing culture solution, filling the culture solution in a fermentation tank, sterilizing, cooling, inoculating, and performing aerated fermentation. By the culture method, a large number of paecilomyces cicadae mycelia and conidia can be generated, and the culture product has the effects of resisting tumors, regulating immunity, reducing blood sugar, blood fat and blood pressure, improving eyesight, resisting radiation, relieving heat and pain, calming and hypnotizing, nourishing and strengthening, improving the renal function and the like, and can be applied to foods, health care products, medicines, cosmetics and the like. The liquid fermentation production process is short in period, high in yield, environment-friendly, stable in product quality and suitable for mass production.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a liquid fermentation method for producing Paecilomyces cicadae mycelium and the application of the culture product. Background technique [0002] Cicada flower, scientific name Paecilomyces cicadae, is the product of certain cicada nymphs parasitized by Paecilomyces cicadae, and is a complex of fungi and insects. Contains glycogen, cordycepic acid, various essential amino acids, D-mannitol, various alkaloids, ergosterol and other active ingredients. The 17 kinds of amino acids, polysaccharides, and mannitol contained in cicadae are similar to those of Cordyceps sinensis, so they are often used as substitutes for Cordyceps sinensis. Cicada has various pharmacological effects such as anti-tumor, regulating immunity, lowering blood sugar, lowering blood fat, lowering blood pressure, improving eyesight, anti-radiation, antipyretic and analgesic, sedative ...

Claims

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Application Information

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IPC IPC(8): C12P1/02A23L1/30A61K36/066A61K8/99A61P35/00A61P37/02A61P3/10A61P3/06A61P9/12A61P27/02A61P17/16A61P25/20A61P29/00A61P13/12C12R1/79
Inventor 陈祝安孙长胜赵伟李成
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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