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Medicament capable of treating liver cancer

A technology for the treatment of liver cancer and drugs, applied in the field of drugs, can solve problems such as damage to normal tissues and cells, and patient intolerance, and achieve the effects of small toxic and side effects, good biocompatibility, and strong anti-tumor effect

Inactive Publication Date: 2011-06-01
郧阳医学院附属人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The treatment of liver cancer mostly adopts a combination of surgical resection, radiotherapy and chemotherapy. Currently, the commonly used chemotherapeutic drugs are cisplatin, doxorubicin or epirubicin, mitomycin, 5-fluorouracil or fluoroglycoside, etc. , these drugs have caused great damage to normal tissues and cells while killing tumor cells, making patients unable to tolerate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Inhibitory effect of chitosan selenite on proliferation of human liver cancer cells cultured in vitro and influence on normal human liver cells.

[0015] The human liver cancer cells SMMC-7721 and normal human liver cells L02 in the logarithmic growth phase were inoculated on a 96-well culture plate. Add 100 μL of chitosan selenite at different concentrations, and do three replicate wells for each concentration; add 100 μL of culture medium to the negative control group, and add 20 μL of 5 mg / mL MTT to each hole after acting for different times (48h and 72h). Continue to culture for 4h, discard the supernatant, and add 150 μL of dimethyl sulfoxide to each well. The absorbance value of each well at 550 nm was measured with a DG-3022 microplate reader (Nanjing, China), and the average value was taken for each group to calculate the inhibition rate. Growth inhibition rate=(blank group optical density-experimental group optical density) / blank group optical densi...

Embodiment 2

[0016] Example 2: Chitosan selenite induces apoptosis of human liver cancer cells cultured in vitro.

[0017] (1) The effect of chitosan selenite on the morphology of human liver cancer cells

[0018] ① Fluorescence microscope observation: liver cancer cells treated with different concentrations of chitosan selenite for 12, 24 and 72 hours were stained with mixed fluorescent dyes of acridine orange (AO) and ethidium bromide (EB), and then inoculated in multiple cells, respectively. The morphological changes of the cells were observed under a fluorescence microscope. Results: Under the microscope, the nucleus or cytoplasm of apoptotic cells in the early and middle stages can be observed to have densely stained granular yellow-green fluorescence, nuclear chromatin condensation, nuclear rupture, and release of apoptotic bodies. Typical morphology of apoptotic cells Change; at the same time, apoptosis has a time- and dose-dependent relationship.

[0019] ② Electron microscope ob...

Embodiment 3

[0023] Example 3: Effect of chitosan selenite on the enzyme activity of liver cancer cells Caspase-3, 8, and 9.

[0024] Collect 3×10 6 Add pre-cooled (4°C) cell lysate (25 μL / 10 6 cells) and mix well, ice bath for 10min and then 10 5 Centrifuge at g for 1 min, draw the supernatant and store it in an ice-water bath tube for the determination of Caspase enzyme activity and protein content. Add 50 μL of supernatant, 50 μL of reaction solution (10 μL of DTT per 1 mL of reaction solution before use), and 5 μL of substrate in a 96-well plate in sequence, incubate at 37°C for 2 h, and measure the optical density at 405 nm with a microplate reader. Set no supernatant wells and negative control wells, and repeat the experiment three times. Results: After 100 and 200 mg / L of chitosan selenite acted on liver cancer cells for 24 hours and 48 hours, the enzyme activities of Caspase-3 and 9 were significantly increased compared with the control group, and the enzyme activities also incr...

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PUM

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Abstract

The invention provides application of chitosan selenite in preparation of a medicament of treating liver cancer. Researches show that selenium is one of necessary trace elements in human bodies and animal bodies, and the selenium has remarkable prevention and treatment effects on multiple tumors. Chitosan [beta (1,4)-2-amino-2-deoxy-D-dextrose] is alkali biological polysaccharide in the natural world, carries positive electricity under the acid condition, can be neutralized with negative ions on the surfaces of tumor cells by adsorption and discharge to resist the growth of the tumor cells, has multiple biological activities such as tumor resistance, inflammation resistance, bacteria resistance and the like, has good biocompatibility, nearly has no toxicity and has no other adverse reactions; and the chitosan selenite is low molecular weight organic selenium prepared by adsorbing selenic acid radicals on chitosan monomers 2-NH2 and 6-OH, has stronger anti-tumor effect, and has lower toxic or side effect.

Description

technical field [0001] The invention relates to a medicine, especially a medicine for treating liver cancer. Background technique [0002] Liver cancer is one of the common malignant tumors in my country, with a high mortality rate, ranking third in the ranking of malignant tumor deaths after stomach and esophagus; in rural areas in some areas, it occupies the second place, second only to gastric cancer. About 110,000 people die from liver cancer every year in my country, accounting for 45% of the world's liver cancer deaths. [0003] The treatment of liver cancer mostly adopts a combination of surgical resection, radiotherapy and chemotherapy. Currently, the commonly used chemotherapeutic drugs are cisplatin, doxorubicin or epirubicin, mitomycin, 5-fluorouracil or fluoroglycoside, etc. , While these drugs kill tumor cells, they also cause great damage to normal tissues and cells, making patients intolerable. Therefore, finding highly efficient, highly selective, and low-t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/722A61P35/00
Inventor 邓守恒王一平陈萍余开峰曾小华曹凤军蔡晓军李林均潘东风李芳
Owner 郧阳医学院附属人民医院
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