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Plasma substitute and preparation method thereof

A technology of plasma substitutes and serum albumin, applied in the field of plasma substitutes and its preparation, to achieve the effects of high blood oxygen content, maintenance of blood volume, and improvement of physical stability

Inactive Publication Date: 2011-03-30
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This new type of plasma substitute is more conducive to solving the problem of hypoxemia caused by a series of diseases such as anemia, fainting, shock, and acute trauma, timely and efficiently replenishing blood oxygen, maintaining blood volume, and restoring normal physiological functions of the body

Method used

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  • Plasma substitute and preparation method thereof
  • Plasma substitute and preparation method thereof
  • Plasma substitute and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of Lipid Carrier Vesicle Suspension in Oxygenated Plasma Substitute

[0031] Weigh 40 mg of hydrogenated soybean lecithin (HSPC), 1000 mg of polyethylene glycol 1500 (PEG1500), and 100 mg of Tween into a 50 ml beaker, add 10 ml of tert-butanol (analytical grade), and dissolve in a water bath at 60° C. by ultrasonication. After the above substances are completely dissolved and the solution becomes clear, it is divided into 10ml vials with a volume of 2ml per bottle, and it is cooled at 0-4°C for more than 30 minutes. The liquid is milky white and solidified, and it is freeze-dried for 40 hours. The freeze-dried sample was filled with perfluoropropane to replace the air in the bottle, and the bottle was sealed tightly.

[0032] Weigh 300mg of hydroxyethyl starch 130 / 0.4 and 45mg of sodium chloride and dissolve in 5ml of distilled water, inject the stopper into the above vial, mix with the freeze-dried sample, and shake to obtain a lipid vesicle susp...

Embodiment 2

[0033] Example 2: Preparation of Albumin Carrier Vesicle Suspension in Oxygenated Plasma Substitute

[0034] Weigh 600mg of hydroxyethyl starch 130 / 0.4 and 90mg of sodium chloride and dissolve them in 10ml of distilled water, add 500mg of bovine serum albumin and mix to prepare a 5% bovine serum albumin solution. Continuously ultrasonically oscillate for 60 s, and a large amount of oxygen is introduced from the bottom of the container at the same time. The gas in the bottle was replaced with oxygen, sealed tightly, and stored in a refrigerator at 4°C. The concentration of microbubbles observed under a microscope is greater than 2.5×10 8 Each / ml, the microbubble diameter is between 2-4μm, and the half-life in vivo is 120min.

Embodiment 3

[0035] Example 3: Preparation of Oxygenated Plasma Substitute Carrier Vesicle Suspension by Spray Drying

[0036] Weigh 0.3g of myristyl phosphatidylcholine (DMPC), 0.02g of myristyl phosphatidylethanolamine (DMPE) and 0.05g of poloxamer (188) and dissolve it in 50ml of phosphate buffer, heat to make it a clear solution . 20ml of perfluorohexane liquid was added at a stirring speed of 800rpm to form micron or nanoscale colostrum including perfluorohexane. The formed colostrum was added with an equal volume of 5% hydroxyethyl starch 40 aqueous solution, stirred at a constant speed of 800 rpm to make it fully mixed, and dried by a spray dryer (temperature controlled at 35° C.) to obtain a blank microbubble powder.

[0037] Take 100 mg of spray-dried blank microbubble powder and pack in 5 ml vials, fill with oxygen to replace the air in the vials, and seal them tightly. When using, inject 2ml of normal saline into the septum plug and shake to obtain lipid vesicle suspension. T...

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Abstract

The invention relates to a plasma substitute and a preparation method thereof. The plasma substitute comprises vesicles, hydroxyethyl starch and an isoosmotic adjusting agent. Blood volume is replenished while the blood oxygen supply is maintained, so that the plasma substitute can be used for treating anaemia, fainting, shock and acute injury.

Description

【Technical field】 [0001] The invention belongs to the field of preparations, and more specifically, the invention relates to a plasma substitute and a preparation method thereof. 【Background technique】 [0002] Appropriate blood oxygen concentration is the basis for maintaining the normal function of the body. Under the hypoxic environment, normal aerobic respiration cannot be carried out in the cells, and the energy released by the decomposition of organic matter will be reduced, which will disturb the physiological process. In many diseases such as ischemic stroke, the patient's brain cells will be permanently damaged due to hypoxia and necrosis. If a certain plasma substitute can send oxygen to the brain in a critical golden time, it can make the brain cells Maintain normal operations and minimize damage. [0003] Existing plasma substitutes include volumetric plasma substitutes, platelet substitutes and red blood cell substitutes. As a volumetric plasma substitute, hy...

Claims

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Application Information

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IPC IPC(8): A61K31/718A61K47/34A61K47/36A61K47/42A61K47/24A61P7/08C12N5/02A61K47/10A61K47/26
Inventor 赵应征李校堃高慧升鲁翠涛周志彩田吉来唐琴琴靳卓徐艳艳张露孙昌正
Owner WENZHOU MEDICAL UNIV
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