Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of live vaccine for gosling plague and live vaccine prepared therefrom

A technology for live goose plague and gosling plague, applied in the field of bioengineering, can solve the problems of irregular, unstable and long time of duck embryo death, and achieve good immune protection effect, prevention of virulent infection, and good stability. Effect

Inactive Publication Date: 2011-02-16
SINOPHARM YANGZHOU VAC BIOLOGICAL ENG CO LTD
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is understood that the death rate of duck embryos inoculated with virus species is only 70-80%; the duck embryos contain low levels of toxicity; the immunized feathers per ml of seedlings are only 1 / 10-1 / 100 of that of goose embryo vaccines; the immune effect is poor
In the middle of 1980s, the applicant eliminated the "duck-embryonized gosling plague live vaccine" that had been developed. The main reasons include: the virus species can only kill 80% of the duck embryos after 25 generations of duck embryos, which is unstable; The death time of duck embryos is irregular and too long; duck embryos have less embryonic liquid, low yield and high cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of live vaccine for gosling plague and live vaccine prepared therefrom
  • Preparation method of live vaccine for gosling plague and live vaccine prepared therefrom
  • Preparation method of live vaccine for gosling plague and live vaccine prepared therefrom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A poison separation

[0024] Diseases: In 1961, the liver, spleen, kidney, pancreas, brain, and hemovirus of the liver, spleen, kidney, pancreas, brain, and blood virus in Yangzhou.

[0025] Disease treatment: Sterile collection of liver, spleen, pancreas, brain and other internal organs such as disease materials and sterile collection of diseased pests, pancreas, spleen and other diseases, cut, grind them, use it, use it, use it, use it, and use it.Sterilizer PBS liquid is diluted by 1: 5, 30 minutes after 3000 rpm / separation, and the clutter's upper liquid is added 4: 1 to analyze the pure chlorine mixing and placed at 37 ° C for 60 minutes, or 4 ° C refrigeratorAfter overnight, after 3000 rpm / separate heart, take the solution and add antibiotics, so that each milliliter contains 1,000 units of penicillin and rinsein, and acts at 37 ° C for 30 minutes.As a virus separation material.

[0026] This method can remove other viruses after chlorine treatment, especially the int...

Embodiment 2

[0050] The SYG61 used by the present invention is Fang Dingyi, Yangzhou University (formerly Su Beinong College) Fang Dingyi, and Professor Wang Yongkun's first reported and separated small goose plague strains in the world in 1961

[0051] Poisonous breeding:

[0052] The poison of the small goose plague virus is the goose embryo urine that is continuously transmitted in the consecutory goose embryo.The poison of the living vaccine for breeding goose is SYG26 ~ 35; the poison of the living vaccine for the manufacture of the chicks and the chicks is SYG41 ~ 50.Add a little sterilized PBS liquid to emulsify the frozen -dried poison, and then dilute it to dilute it by 1: 100.It may be preserved in the velvet urine poison of ~ 15 ° C as a sterilized PBS liquid to dilute 1: 100.The diluted poisons were vaccinated with 10 ~ 20 days of age, and they were easy to feel goose embryo, with 0.2ml of velvet urine cavity.Select the death of 48 ~ 120 hours of vaccination, and the typical goose ...

Embodiment 3

[0076] Test of the Protection Valley Protection Vaccine:

[0077] Vaccium: 5 batches of frozen dry seedlings including 0607, 0608, 0609, 0901, and 0902, each bottle of seedlings is 500 doses, and stored in the refrigerator of ~ 15 ° C.

[0078] Chuangni: A 2 -day -old chick with non -epidemic areas, immune, and aggressive goose group.

[0079] Attacking toxicity: Syg61 poison strain.For one -day dystrophic toxic power, each ml should be ≥10 6 LD50.

[0080] 0607, 0608, 0609, including 3 batches of freezing dried seedlings, each batch of 5 bottles, each bottle with 1.0ml sterilized physiological saline dissolves, and the amount of seedlings such as extraction is mixed for mixing.A total of 13010 immune three groups, each with 1:50 seedlings within 48 hours after the shells, 0.1ml of subcutaneous injection, and breeding for 9 days.In 176, only SYG61 strain was attacked, and 10 of the same age chicks were set up for control groups. Each subcutaneous injection was diluted by 0.5ml, 10...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a live vaccine for gosling plague and a live vaccine prepared therefrom. The preparation method of the live vaccine for the gosling plague comprises the steps of screening the breeding virus SYG61 of the gosling plague; diluting the breeding virus of the gosling plague viruses by utilizing sterilizing PBS (Phosphate Buffer Solution) in the ratio of 1 to 100; inoculating a well-developed goose embryo with 12 days, wherein each embryo flocking urine cavity is 0.2ml; the goose embryo dying in 48 to 144 hours is placed into a refrigerator with 2 to 8 DEG C for 4 to 24 hours; soaking the cooled goose embryo into a 5% bromo-geramine disinfectant for 5 to 10 minutes; placing into a sterile chamber after wiping drily; disinfecting a gas chamber eggshell part by utilizing 5% iodine tincture; opening the eggshell in a sterile procedure; eliminating an egg membrane and tearing a flocking urine membrane; sucking allantoic fluid and amniotic fluid; adding 5% sucrose skimmed milk as a stabilizing agent; simultaneously adding 1000 units / ml of penicillin and streptomycin respectively; fully agitating; quantitatively subpackaging; and rapidly refrigerating and drying in a vacuum state after subpackaging. The preparation method of the live vaccine for the gosling plague of the invention has the advantages of fewer steps and easy operation. Moreover, the prepared live vaccine for the gosling plague has the advantages of high yield, good stability, low cost and good immunity protection effect.

Description

Technical field [0001] The invention is a biological engineering technology field, which involves the preparation method of small goose plague vaccines and the live vaccine they prepared. Background technique [0002] Goose plague is the acute or subacute semi -infectious diseases of the glutinous roses within 20 days caused by the small goose plague virus. Its incidence and mortality can be as high as 90 ~ 100 %.EssenceIn 1956, Professor Fang Yiyi first discovered the disease in Yangzhou. In 1961, Fang Yi, Wang Yongkun, etc. discovered the disease again in Yangzhou, and was separated from the virus. It was named the small goose plague.This disease has an outbreak in various provinces and world -raising countries across the country. [0003] According to the International Veterinary Bureau (OIE) International Committee, mammals, poultry and bee A, and B diseases edited by the OIE Standard Committee "Diagnostic Test and Vaccine Standard Manual" (third edition, 1996)The content and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/23A61P31/20
CPCY02A50/30
Inventor 张建军周玉双尹松涛陈清顾成钢
Owner SINOPHARM YANGZHOU VAC BIOLOGICAL ENG CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com